16S rRNA squencing to identify microbiomes associated with different crops and different growing conditions
- Laboratoire Ecologie Microbienne at the University of Lyon, France
The microbiome associated with plant roots, the rhizobiome, can harbor beneficial microbes that alleviate stress, but the factors influencing their recruitment are unclear. We conducted a greenhouse experiment using field soil with a legacy of growing switchgrass and common bean to investigate the impact of short-term drought severity on the recruitment of active bacterial rhizobiome members. We applied 16S rRNA and 16S rRNA gene sequencing for both crops and metabolite profiling for switchgrass. We included planted and unplanted conditions to distinguish environment- versus plant-mediated rhizobiome drivers.
- Research Organization:
- Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- DOE Contract Number:
- SC0018409
- OSTI ID:
- 3003596
- Country of Publication:
- United States
- Language:
- English
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Disentangling plant- and environment-mediated drivers of active rhizosphere bacterial community dynamics during short-term drought
Journal Article
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Sat Jul 27 00:00:00 EDT 2024
· Nature Communications
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OSTI ID:2406988