The stem cell-type transcriptome of bioenergy sorghum reveals the spatial regulation of secondary cell wall networks  

Dataset · Thu Feb 08 23:00:00 EST 2024

DOI:https://doi.org/10.82250/3003569· OSTI ID:3003569

Mullet, John ^[1]

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1. GLBRC - Texas A&M University

Stems accumulate high levels of sucrose that could be used to synthesize bioethanol and useful biopolymers if information about stem cell-type gene expression and regulation was available to enable engineering. To obtain this information, Laser Capture Microdissection (LCM) was used to isolate and collect transcriptome profiles from five major cell types that are present in stems of the sweet sorghum Wray. Transcriptome analysis identified genes with cell-type specific and cell-preferred expression patterns that reflect the distinct metabolic, transport, and regulatory functions of each cell type.

• Research Organization: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Biological and Environmental Research (BER)
• DOE Contract Number: SC0018409
• OSTI ID: 3003569
• Country of Publication: United States
• Language: English

Related Subjects

cell specificity
gene regulatory network
laser capture microdissection
secondary cell wall
sorghum
stem