Cellulose Nanocrystals are a Renewable and Biocompatible Nanocarrier of Agrochemicals Directly to Plant Cells

Vinzant, Kari; Clouse, Delaney E.; Amit, Sadat Kamal; Ivanov, Ilia N.; Davis, Virginia A.; Khodakovskaya, Mariya V.

doi:10.1002/adsu.202300511

Cellulose Nanocrystals are a Renewable and Biocompatible Nanocarrier of Agrochemicals Directly to Plant Cells

Journal Article · Tue Dec 19 00:00:00 EST 2023 · Advanced Sustainable Systems

DOI:https://doi.org/10.1002/adsu.202300511· OSTI ID:3002941

Vinzant, Kari ^[1]; Clouse, Delaney E. ^[2]; Amit, Sadat Kamal ^[2]; ^[3]; Davis, Virginia A. ^[2]; ^[1]

Univ. of Arkansas, Little Rock, AR (United States)
Auburn Univ., AL (United States)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)

More sustainable agricultural practices are critical to meeting humanity's food needs while minimizing adverse environmental impacts. Engineered nanomaterials used as nanocarriers promise to reduce the volume of agrochemicals required for crop production but concerns about nanocontamination of agricultural products necessitate the discovery of naturally occurring alternatives. Here, in this study, plant‐derived cellulose nanocrystals (CNC) as a “green” alternative to man‐made nanomaterials for the economical delivery of agrochemicals to plants are suggested. Using confocal microscopy and fluorescently labeled CNC, it is demonstrated that CNC can successfully penetrate the plant cell wall and enter cells without causing any negative effects on the overall plant phenotype, genome, and metabolome. The efficiency of CNC to deliver chemicals is demonstrated through in vitro tests involving CNC covalently conjugated with 2,4‐dichlorophenoxyacetic acid (2,4‐D). Plant cell culture experiments confirmed that CNC can be used as a 2,4‐D nanocarrier and reduces the volume of plant growth regulators needed to cultivate plant cells. This work creates a new platform for cyclical agricultural practices where CNC extracted from agricultural waste can be used for the direct delivery of agrochemicals to crops, thereby reducing the environmental burden created by both agricultural waste and excess herbicides.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDA; USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 3002941

Journal Information:: Advanced Sustainable Systems, Journal Name: Advanced Sustainable Systems Journal Issue: 5 Vol. 8; ISSN 2366-7486

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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