From cytoplasm to lumen—mapping the free pools of protein subunits of three photosynthetic complexes using quantitative mass spectrometry

Williams, Anna M.; Jackson, Philip J.; Theg, Steven M.; Bricker, Terry M.; Hunter, C. Neil; Liu, Haijun

doi:10.1002/1873-3468.70029

From cytoplasm to lumen—mapping the free pools of protein subunits of three photosynthetic complexes using quantitative mass spectrometry

Journal Article · Wed Mar 12 00:00:00 EDT 2025 · FEBS Letters

DOI:https://doi.org/10.1002/1873-3468.70029· OSTI ID:3000717

^[1]; ^[2]; ^[3]; Bricker, Terry M. ^[4]; ^[2]; ^[1]

Saint Louis University, MO (United States)
University of Sheffield (United Kingdom)
University of California, Davis, CA (United States)
Louisiana State University, Baton Rouge, LA (United States)

The phycobilisome (PBS) captures light energy and transfers it to photosystem I (PSI) and photosystem II (PSII). Which and how many copies of protein subunits in PBSs, PSI, and PSII remain unbound in thylakoids are unknown. Here, quantitative mass spectrometry (QMS) was used to quantify substantial pools of free extrinsic subunits of PSII and PSI. Interestingly, the membrane intrinsic PsaL is 3-fold higher than PsaA/B. This scenario complements the static structures of these complexes as revealed by X-ray crystallography and cryo-EM. Furthermore, the ratios of ApcG and photoprotective OCP over PBS indicate a pool of extra ApcG. The 2.5 ratio of CpcG-PBS over CpcL-PBS improves our understanding of these light-harvesting complexes involved in energy capture and photoprotection in cyanobacteria.

View Accepted Manuscript (DOE)

Research Organization:: Saint Louis University, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); Biotechnology and Biological Sciences Research Council UK; European Research Council Synergy

Grant/Contract Number:: SC0025019; SC0020304

OSTI ID:: 3000717

Journal Information:: FEBS Letters, Journal Name: FEBS Letters Journal Issue: 10 Vol. 599; ISSN 0014-5793; ISSN 1873-3468

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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From cytoplasm to lumen—mapping the free pools of protein subunits of three photosynthetic complexes using quantitative mass spectrometry

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