Selective adsorption/recovery of Pb, Cu, and Cd with multiple fixed beds containing immobilized bacterial biomass
- Feng Chia Univ., Taichung (Taiwan, Province of China). Dept. of Chemical Engineering
Fixed-bed columns packed with calcium alginate (CA)-immobilized biomass of Pseudomonas aeruginosa PU21 were utilized to remove lead (Pb), copper (Cu), and cadmium (Cd) from the contaminated water. In the absence of competing metals, saturano capacity of CA-immobilized cells in batch operations was 1.60, 2.42, and 1.06 mmol/g, for Pb, Cu, and Cd, respectively. The Langmuir constants (K) obtained from the langmuir isotherm were 157.6, 4.2, and 3.7 mM{sup {minus}1} for Pb, Cu, and Cd, respectively. Results from single-metal biosorption with 10-cm immobilized-cell columns show that, for an influent metal concentration of 193 {micro}M, the total capacities for Pb, Cu, and Cd, respectively, were 5.12, 4.03, and 3.48 mmol, which is nearly 25--30% higher than those obtained from columns containing only cell-free CA matrix. With the influent containing ternary mixtures of Pb, Cu, and Cd, columns with immobilized cells exhibited predominant selectivity to Pb, whereas in the cell-free columns, the dominance of Pb adsorption reduced, along with an appreciable increases in the adsorption of Cu. The metal-laden columns were regenerated by elution with HCl solution (pH 2.0). The metal recovery ratios were 80:1, 60:1, and 27:1 for Cu, Cd, and Pb, respectively. Moreover, with a pH gradient elution, the column-trapped metals can be optimally recovered at distinct pH values. Continuous biosorption of Pb, Cu, and Cu with four columns in series was also conducted.
- OSTI ID:
- 290116
- Journal Information:
- Biotechnology Progress, Journal Name: Biotechnology Progress Journal Issue: 5 Vol. 14; ISSN 8756-7938; ISSN BIPRET
- Country of Publication:
- United States
- Language:
- English
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