Hg{sup 2+} removal by genetically engineered Escherichia coli in a hollow fiber bioreactor
- Cornell Univ., Ithaca, NY (United States)
Escherichia coli cells engineered to express an Hg{sup 2+} transport system and metallothionein accumulated Hg{sup 2+} effectively over a concentration range of 0.2--4 mg/L in batch systems. Bioaccumulation was selected against other metal ions and resistant to changes in ambient conditions such as pH, ionic strength, and the presence of common metal chelators or complexing agents. Here the authors report the characterization of the bioaccumulation system based on its kinetics and an isotherm. Bioaccumulation was rapid and followed Michaelis-menten kinetics. A hollow fiber bioreactor was constructed to retain the genetically engineered cells. The bioreactor was capable of removing and recovering Hg{sup 2+} effectively at low concentrations, reducing a 2 mg/L solution to about 5 {micro}g/L. A mathematical equation that quantitatively described Hg{sup 2+} removal by the bioreactor provides a basis for the optimization and extrapolation of the bioreactor. The genetically engineered E. coli cells and the bioreactor system have excellent properties for bioremediation of Hg{sup 2+}-contaminated environments.
- OSTI ID:
- 290114
- Journal Information:
- Biotechnology Progress, Journal Name: Biotechnology Progress Journal Issue: 5 Vol. 14; ISSN 8756-7938; ISSN BIPRET
- Country of Publication:
- United States
- Language:
- English
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