The human C/EBP{delta} (CRP3/CELF) gene: Structure and chromosomal localization

Journal Article · Sat May 01 00:00:00 EDT 1993 · Genomics

DOI:https://doi.org/10.1006/geno.1993.1220· OSTI ID:28981

Cleutjens, C B.J.M.; Dekken, H van; Trapman, J; Smit, E M.E.; Hagemeijer, A ^[1]; Wagner, M J; Wells, D E ^[2]

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1. Erasmus Univ., Rotterdam (Netherlands)
2. Univ. of Houston, TX (United States)

In an attempt to identify C/EBP-like transcription factors expressed in the prostate, a cDNA homologous to the mouse C/EBP{delta} (CRP3) and the rat CELF gene was isolated. A genomic clone containing the entire C/EBP{delta} gene was isolated using a cDNA fragment as a probe. The gene was characterized by restriction mapping and sequence analysis. By fluorescence in situ hybridization, using the biotinylated genomic clone as a probe, the C/EBP{delta} gene was assigned to the pericentromeric region of human chromosome 8, most probably to 8q11. This chromosomal localization was confirmed by analysis of a panel of human {times} hamster somatic cell hybrid DNA samples with a C/EBP{delta}-specific STS. As a result, the C/EBP{delta} gene could be positioned between the PLAT and the MOS loci. 24 refs., 3 figs.

• OSTI ID: 28981
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 16; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 8
TRANSCRIPTION FACTORS