Modulation of the phosphate-starvation response in Escherichia coli by genetic manipulation of the polyphosphate pathways
- Univ. of California, Berkeley, CA (United States). Dept. of Chemical Engineering
The effect of intracellular polyphosphate on the phosphate-starvation response in Escherichia coli was studied by genetically manipulating the intracellular polyphosphate levels and by performing phosphate shifts on the genetically engineered strains. Strains that produced large quantities of polyphosphate and were able to degrade it induced the phosphate-starvation response to a lesser extent than wild-type strains, whereas strains that were unable to degrade a large intracellular polyphosphate pool induce the phosphate-starvation response to a greater extent than wild-type strains. These results have important implications for expression of heterologous genes under control of the phoA promoter.
- OSTI ID:
- 287545
- Journal Information:
- Biotechnology and Bioengineering, Journal Name: Biotechnology and Bioengineering Journal Issue: 4 Vol. 51; ISSN 0006-3592; ISSN BIBIAU
- Country of Publication:
- United States
- Language:
- English
Similar Records
Optimization of polyphosphate degradation and phosphate secretion using hybrid metabolic pathways and engineered host strains
Genetic manipulation of polyphosphate metabolism affects cadmium tolerance in Escherichia coli