Detection of petroleum hydrocarbons in soil and water by immunoassay
- Ohmicron Corp., Newtown, PA (United States)
A magnetic particle based enzyme immunoassay (EIA) that detects small aromatic hydrocarbons was developed. This EIA can be used to directly test water samples or can be coupled with a simple extraction method to identify the presence of petroleum hydrocarbons in soil. This immunoassay offers several advantages over traditional testing methods (i.e. GC) including speed, cost effectiveness and portability. This assay can be performed on site in less than one hour. The assay`s performance with soil and water samples was evaluated in several studies. In one study conducted on water samples from locations across the US, recoveries of spiked Total BTEX averaged greater than 99% with results ranging from 87% to 119%; one false positive (1.8%) was observed. Soil samples spiked with Total BTEX at concentrations ranging from 0.25 to 10 ppm were extracted, diluted and evaluated in the immunoassay. Recoveries averaged 113% with results ranging from 104% to 120%. In a third study, soil samples collected from various remediation sites were extracted and run in the immunoassay. The assay and recommended extraction procedure agreed well with results obtained by EPA Method 8020 in determining the presence and degree of contamination. Additional study results and data on the cross-reactivity of the assay for various small aromatic hydrocarbons and petroleum fuel mixtures (i.e. gasoline) are also presented.
- OSTI ID:
- 276366
- Report Number(s):
- CONF-950209--
- Country of Publication:
- United States
- Language:
- English
Similar Records
Evaluation of immunoassay-based field test kits for the detection of petroleum fuel hydrocarbons in soil
Evaluation of immunoassay-based field test kits for the detection of petroleum fuel hydrocarbons in soil