Construction of a 3.7-Mb physical map within human chromosome 20p12 ordering 18 markers in the alagille syndrome locus

Journal Article · Sat Jun 10 04:00:00 EDT 1995 · Genomics

DOI:https://doi.org/10.1006/geno.1995.1078· OSTI ID:273436

Pollet, N; Dhorne-Pollet, S; Deleuze, J F ^[1]

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1. INSERM, Le Kremlin-Bicetre (France); and others

Alagille syndrome (AGS, MIM 118450) is associated with human chromosome band 20p12. To study this region, we constructed a 3.7-Mb physical map using 36 YACs isolated from the CEPH YAC library with three sequence-tagged sites (STS): D20S503, D20S41, and D20S188. New STSs were obtained from 6 isolated YAC end-fragments. Eighteen markers were ordered on the contig as follows: 20pte1-D20S177-D20S175-D20S509-D20S5/D20S503-D20S506-D20S162-D20S504-D20S505-D20S507-D20S188-(D20S6-D20S27-D20S189)-D20S186-D20S41-D20S61-D20S492-D20S508-20pcen. A restriction map with the enzymes AscI, M{ell}uI, NotI, SacII, and SfiI was generated, revealing seven putative CpG islands. We established a YAC contig that spans the AGS region and thus will be valuable for cloning candidate genes and searching for DNA polymorphisms segregating with this syndrome. 45 refs., 3 figs., 2 tabs.

• OSTI ID: 273436
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 27; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CHROMOSOMAL ABERRATIONS
CONTIGS
DESIGN
DIGESTIVE SYSTEM DISEASES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
DOMINANT MUTATIONS
FLUORESCENCE
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOMES
INFANTS
MUTATIONS
NUCLEOTIDES
PHENOTYPE
POLYMERASE CHAIN REACTION
PROBES