Phytotoxicity, uptake and metabolism of 1,4-dichlorobenzene by plant cells

Journal Article · Mon Jul 01 04:00:00 EDT 1996 · Environmental Toxicology and Chemistry

DOI:https://doi.org/10.1897/1551-5028(1996)015<1109:PUAMOD>2.3.CO;2· OSTI ID:268015

Wang, M J ^[1]; Bokern, M; Boehme, C; Harms, H ^[2]; Jones, K C ^[1]

---

1. Lancaster Univ. (United Kingdom). Inst. of Environmental and Biological Sciences
2. Federal Agricultural Research Center, Braunschweig (Germany). Inst. of Plant Nutrition and Soil Science

Phytotoxicity, uptake, and metabolism of 1,4-dichlorobenzene (1,4-DCB) by carrot (Daucus carota L.), soybean (Glycine max. L.), tomato (Lycopersicon esculentum Mill.), and red goosefoot (Chenopodiun rubrum L.) cell suspension cultures were studied. Sealed glass systems were utilized for the investigation because 1,4-DCB is volatile. The sealed systems affect the growth of plant cells, but do not provide different results when testing xenobiotic uptake and metabolism. 1,4-Dichlorobenzene (40 {micro}g in 40 ml medium) was taken up by carrot (49%), soybean (50%), and red goosefoot (62%) cells. Only the soybean cell cultures provided evidence of the existence of metabolites of this compound, probably conjugates of chlorophenols. Conditions for phytotoxicity tests were modified because the growth of cell cultures was affected when sealed for longer than 2 d. 1,4-Dichlorobenzene is toxic to cell cultures of the three tested plant species (tomato, soybean, and carrot). Concentrations of 0.5 mM caused 50% growth inhibition in carrot and soybean cultures. The tomato cultures were more sensitive, with 0.05 mM causing 50% growth inhibition.

• Sponsoring Organization: USDOE
• OSTI ID: 268015
• Journal Information: Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Journal Issue: 7 Vol. 15; ISSN ETOCDK; ISSN 0730-7268
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
CARROTS
CHLORINATED AROMATIC HYDROCARBONS
GENETIC VARIABILITY
GLYCINE HISPIDA
PLANT GROWTH
PLANTS
ROOT ABSORPTION
SENSITIVITY
TOMATOES
TOXICITY