Cloning and chromosomal mapping of three novel genes, GPR9, GPR10, GPR14, encoding receptors related to interleukin 8, neuropeptide Y, and somatostatin receptors
- Univ. of Toronto, Ontario (Canada); and others
We employed the polymerase chain reaction and genomic DNA library screening to clone novel human genes, GPR9 and GPR10, and a rate gene, GPR14. GPR9, GPR10 and GPR14 are intronless within their coding regions, while GPR9 contains at least one intron. The receptor encoded by GPR9 shares the highest identity with human IL-8 receptor type B (38% overall and 53% in the transmembrane regions), followed by IL-8 receptor type A (36% overall and 51% in the transmembrane domains). GPR10 encodes a receptor that shares highest identity with the neuropeptide Y-receptor (31% overall and 46% in the transmembrane domains). The receptor encoded by GPR14 shares highest identity with the somatostatin receptor SSTR 4 (27% overall and 41% in the transmembrane domains). Fluorescence in situ hybridization analysis localized GPR9 to chromosome 8p11.2-p12 and GPR10 to chromosome 10q25.3-q26. 22 refs., 3 figs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 258257
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 29; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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