Increased inflammation as well as decreased endoplasmic reticulum stress and translation differentiate pancreatic islets from donors with pre-symptomatic stage 1 type 1 diabetes and non-diabetic donors

Journal Article · Mon Jun 02 00:00:00 EDT 2025 · Diabetologia

DOI:https://doi.org/10.1007/s00125-025-06417-3· OSTI ID:2570268

Swensen, Adam C. ^[1]; Piehowski, Paul D. ^[1]; Chen, Jing ^[2]; Chan, X’avia Y. ^[1]; Kelly, Shane S. ^[1]; Petyuk, Vladislav A. ^[1]; Moore, Ronald J. ^[1]; Nasif, Lith ^[2]; Butterworth, Elizabeth A. ^[2]; Atkinson, Mark A. ^[2]; Kulkarni, Rohit N. ^[3]; Campbell-Thompson, Martha ^[2]; Mathews, Clayton E. ^[2]; Qian, Wei-Jun ^[1]

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1. Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
2. Univ. of Florida, Gainesville, FL (United States)
3. Beth Israel Deaconess Medical Center, Boston, MA (United States). Joslin Diabetes Center; Harvard Medical School, Boston, MA (United States)

Aims/hypothesis Progression to type 1 diabetes is associated with genetic factors, the presence of autoantibodies and a decline in beta cell insulin secretion in response to glucose. Very little is known regarding the molecular changes that occur in human insulin-secreting beta cells prior to the onset of type 1 diabetes. Herein, we applied an unbiased proteomics approach to identify changes in proteins and potential mechanisms of islet dysfunction in islet-autoantibody-positive organ donors with pre-symptomatic stage 1 type 1 diabetes (HbA1c ≤42 mmol/mol [6.0%]). We aimed to identify pathways in islets that are indicative of beta cell dysfunction. Methods Multiple islet sections were collected through laser microdissection of frozen pancreatic tissues from organ donors positive for single or multiple islet autoantibodies (AAb⁺, n=5), and age (±2 years)- and sex-matched non-diabetic (ND) control donors (n=5) obtained from the Network for Pancreatic Organ donors with Diabetes (nPOD). Islet sections were subjected to MS-based proteomics and analysed with label-free quantification followed by pathway and functional annotations. Results Analyses resulted in ~4500 proteins identified with low false discovery rate (<1%), with 2165 proteins reliably quantified in every islet sample. We observed large inter-donor variations that presented a challenge for statistical analysis of proteome changes between donor groups. We therefore focused on only the donors with stage 1 type 1 diabetes who were positive for multiple autoantibodies (mAAb⁺, n=3) and genetic risk compared with their matched ND controls (n=3) for the final statistical analysis. Approximately 10% of the proteins (n=202) were significantly different (unadjusted p<0.025, q<0.15) for mAAb⁺ vs ND donor islets. The significant alterations clustered around major functions for upregulation in the immune response and glycolysis, and downregulation in endoplasmic reticulum (ER) stress response as well as protein translation and synthesis. The observed proteome changes were further supported by several independent published datasets, including a proteomics dataset from in vitro proinflammatory cytokine-treated human islets and single-cell RNA-seq datasets from AAb⁺ individuals. Conclusions/interpretation In situ human islet proteome alterations in stage 1 type 1 diabetes centred around several major functional categories, including an expected increase in immune response genes (elevated antigen presentation/HLA), with decreases in protein synthesis and ER stress response, as well as compensatory metabolic response. The dataset serves as a proteomics resource for future studies on beta cell changes during type 1 diabetes progression and pathogenesis. Data availability The LC-MS raw datasets that support the findings of this study have been deposited in the online repository: MassIVE (https://massive.ucsd.edu/ProteoSAFe/static/massive.jsp) with accession no. MSV000090212.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
• Sponsoring Organization: National Institutes of Health (NIH); USDOE
• Grant/Contract Number: AC05-76RL01830
• OSTI ID: 2570268
• Report Number(s): PNNL-SA--203684
• Journal Information: Diabetologia, Journal Name: Diabetologia Journal Issue: 7 Vol. 68; ISSN 0012-186X; ISSN 1432-0428
• Publisher: Springer Science and Business Media LLC
• Country of Publication: United States
• Language: English

Related Subjects

Autoantibody-positive
Beta cells
Human islets
Islet proteome
Laser microdissection
Mass spectrometry
NPOD
Prediabetes
Proteomics