Metagenome-assembled genomes measured at 3 depths during snowmelt period in East River, CO (March, May, and June, September 2017)
- Lawrence Berkeley National Laboratory
- University of Rhode Island - Kingston
- University of California Berkeley
- U.S. Environmental Protection Agency
- Oregon State University
- Pacific Northwest National Laboratory (PNNL)
- Joint Gen
- University of Arizona
- School of Ecology and Environment, Northwestern Polytechnical University
Snowmelt is a critical biogeochemical period that accounts for large nitrogen (N) export events from high-elevation watersheds. Soil microbial populations bloom and immobilize N during snowmelt, yet the population size crashes in spring, which releases a pulse of soil N. We sought to discover the N sources fueling this microbial bloom and determine the fate of N following microbial die-off. Here, focusing on the snowmelt period within a headwater catchment of the Upper Colorado River Basin (East River, CO), we deployed strain-resolved metagenomics to identify the metabolic pathways and processes that mobilize soil N during and after snowmelt. Soil metagenome samples were taken from 6 snowpits from 3 depths (0-5cm, 5-15cm, >15cm) at 4 time points during snowmelt period (March 2017, May 2017, and June 2017, September 2017) generating 48 metagenomes. We reconstructed 474 metagenome-assembled genomes (MAGs) across all metagenomes.All 48 metagenomes were sequenced at JGI and raw data can be found under JGI (Joint Genome Institute) GOLD Study Gs0135149. Metagenome assemblies from IMG under the same study were used for genome binning. This dataset (1) a zip file of 474 MAGs (as fasta files, Gs0135149_bins_tar.gz), (2) sample metadata file with sample IGSNs (International Generic Sample Numbers) (samples.csv), (3) bounding box coordinates for the sampled locations (Gs0135149.kml), (4) metagenome metadata file listing IMG/M (Integrated Microbial Genomes/Metagenomes) metagenome accessions linking samples to metagenomes (metagenomes.csv), (5) location metadata file (locations.csv), (6) file-level metadata file (flmd.csv) and (7) data dictionary (dd.csv) file.This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.
- Research Organization:
- Watershed Function SFA
- Sponsoring Organization:
- U.S. DOE > Office of Science > Biological and Environmental Research (BER)
- DOE Contract Number:
- AC02-05CH11231
- OSTI ID:
- 2569158
- Country of Publication:
- United States
- Language:
- English
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EARTH SCIENCE > BIOSPHERE > OMICS > BUILDING BLOCKS > CONTIG:GCMD
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EARTH SCIENCE > LAND SURFACE > SOILS
ESS-DIVE CSV File Formatting Guidelines Reporting Format
ESS-DIVE File Level Metadata Reporting Format
ESS-DIVE Location Metadata Reporting Format
ESS-DIVE Sample ID and Metadata Reporting Format