Integrated Proteomic and Glycoproteomic Characterization of Human High-Grade Serous Ovarian Carcinoma

Hu, Yingwei; Pan, Jianbo; Shah, Punut; Ao, Minghui; Thomas, Stefani N.; Liu, Yang; Chen, Lijun; Schnaubelt, Michael; Clark, David J.; Rodriguez, Henry; Boja, Emily; Hiltke, Tara R.; Kinsinger, Christopher; Rodland, Karin D.; Li, Qing Kay; Qian, Jiang; Zhang, Zhen; Chan, Daniel W.; Zhang, Hui

doi:10.1016/j.celrep.2020.108276

Integrated Proteomic and Glycoproteomic Characterization of Human High-Grade Serous Ovarian Carcinoma

Journal Article · Tue Oct 20 04:00:00 EDT 2020 · Cell Reports

DOI:https://doi.org/10.1016/j.celrep.2020.108276· OSTI ID:2565841

Hu, Yingwei ^[1]; Pan, Jianbo ^[2]; Shah, Punut ^[2]; Ao, Minghui ^[2]; Thomas, Stefani N. ^[3]; Liu, Yang ^[2]; Chen, Lijun ^[2]; Schnaubelt, Michael ^[2]; Clark, David J. ^[2]; Rodriguez, Henry ^[4]; Boja, Emily ^[5]; Hiltke, Tara R. ^[6]; Kinsinger, Christopher ^[7]; ^[8]; Li, Qing Kay ^[2]; Qian, Jiang ^[2]; Zhang, Zhen ^[2]; Chan, Daniel W. ^[2]; Zhang, Hui ^[2]

Johns Hopkins School of Medicine
Johns Hopkins University
University of Maryland
National Cancer Institute
National Cancer Institute,NIH Bethesda, Maryland
National Cancer Institute, Bethesda, MD
National Cancer Institute, National Institutes of Health
BATTELLE (PACIFIC NW LAB)

Many gene products exhibit great structural heterogeneity because of an array of modifications. These modifications are not directly encoded in the genomic template but often affect the functionality of proteins. Protein glycosylation plays a vital role in proper protein functions. However, the analysis of glycoproteins has been challenging compared with other protein modifications, such as phosphorylation. Here, we perform an integrated proteomic and glycoproteomic analysis of 83 prospectively collected high-grade serous ovarian carcinoma (HGSC) and 23 non-tumor tissues. Integration of the expression data from global proteomics and glycoproteomics reveals tumor-specific glycosylation, uncovers different glycosylation associated with three tumor clusters, and identifies glycosylation enzymes that were correlated with the altered glycosylation. In addition to providing a valuable resource, these results provide insights into the potentialroles of glycosylation in the pathogenesis of HGSC, with the possibility of distinguishing pathological outcomes of ovarian tumors from non-tumors, as well as classifying tumor clusters.

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 2565841

Report Number(s):: PNNL-SA-160481

Journal Information:: Cell Reports, Journal Name: Cell Reports Journal Issue: 3 Vol. 33

Country of Publication:: United States

Language:: English

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