Metabolic Interactions between Brachypodium and Pseudomonas fluorescens under Controlled Iron-Limited Conditions

Boiteau, Rene M.; Markillie, Lye Meng; Mitchell, Hugh D.; Hu, Dehong; Chu, Rosalie K.; Hoyt, David W.; Pasa Tolic, Ljiljana; Jansson, Georg C.; Jansson, Janet K.

doi:10.1128/mSystems.00580-20

Metabolic Interactions between Brachypodium and Pseudomonas fluorescens under Controlled Iron-Limited Conditions

Journal Article · Mon Jan 04 23:00:00 EST 2021 · mSystems

DOI:https://doi.org/10.1128/mSystems.00580-20· OSTI ID:2565409

Boiteau, Rene M. ^[1]; Markillie, Lye Meng ^[2]; Mitchell, Hugh D. ^[2]; ^[2]; Chu, Rosalie K. ^[2]; ^[2]; Pasa Tolic, Ljiljana ^[2]; Jansson, Georg C. ^[2]; Jansson, Janet K. ^[2]

OREGON STATE UNIVERSITY
BATTELLE (PACIFIC NW LAB)

Plant iron (Fe) nutritional status has a significant impact on rhizosphere microbial communities. While this effect has been attributed to alterations in the composition of root exudates, the underlying mechanisms are not known. Here, we investigated the effect of Fe deficiency on the interactions between the grass Brachypodium distachyon and the common soil bacteria Pseudomonas fluorescens SBW25. Increased phytosiderophore production was the most significant response of Brachypodium root exudation to Fe deficiency. We observed upregulated expression of phytosiderophore biosynthesis genes but lower accumulated exudate concentrations in the presence of Pseudomonas, indicating that the bacteria degrade these phytosiderophores. Pseudomonas did not produce endogenous siderophores under the low Fe conditions. Rather, the bacterial transcriptome revealed upregulation of four genes in response to Fe deficiency that contain motifs associated with cellular uptake of small molecules. Collectively, these results suggest that rhizosphere Pseudomonas fluorescens may take up phytosiderophores produced by grasses in response to Fe deficiency, and we propose target genes that may be involved. Our findings provide insight into the molecular basis for the exchange of C, N, and Fe between plants and bacteria under Fe deficient conditions. These interactions may contribute to differences in the rhizosphere microbial community structure across soil pH regimes.

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 2565409

Report Number(s):: PNNL-SA-147916

Journal Information:: mSystems, Journal Name: mSystems Journal Issue: 1 Vol. 6

Country of Publication:: United States

Language:: English

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Metabolic Interactions between Brachypodium and Pseudomonas fluorescens under Controlled Iron-Limited Conditions

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