Spatial Proteomics towards cellular Resolution

Kwon, Yumi; Fulcher, James M.; Paša-Tolić, Ljiljana; Qian, Wei-Jun

doi:10.1080/14789450.2024.2445809

Spatial Proteomics towards cellular Resolution

Journal Article · Wed Dec 25 00:00:00 EST 2024 · Expert Review of Proteomics

DOI:https://doi.org/10.1080/14789450.2024.2445809· OSTI ID:2564693

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Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Introduction: Spatial biology is an emerging interdisciplinary field facilitating biological discoveries through the use of spatial omics technologies. Recent advancements in spatial transcriptomics, spatial genomics (e.g. genetic mutations and epigenetic marks), multiplexed immunofluorescence, and spatial metabolomics/lipidomics have enabled high-resolution spatial profiling of gene expression, genetic variation, protein expression, and metabolites/lipids profiles in tissue. These developments contribute to a deeper understanding of the spatial organization within tissue microenvironments at the molecular level. Areas covered: This report provides an overview of the untargeted, bottom-up mass spectrometry (MS)-based spatial proteomics workflow. It highlights recent progress in tissue dissection, sample processing, bioinformatics, and liquid chromatography (LC)-MS technologies that are advancing spatial proteomics toward cellular resolution. Expert opinion: The field of untargeted MS-based spatial proteomics is rapidly evolving and holds great promise. To fully realize the potential of spatial proteomics, it is critical to advance data analysis and develop automated and intelligent tissue dissection at the cellular or subcellular level, along with high-throughput LC-MS analyses of thousands of samples. In conclusion, achieving these goals will necessitate significant advancements in tissue dissection technologies, LC-MS instrumentation, and computational tools.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 2564693

Report Number(s):: PNNL-SA--203070

Journal Information:: Expert Review of Proteomics, Journal Name: Expert Review of Proteomics Journal Issue: 12 Vol. 21; ISSN 1478-9450

Publisher:: Taylor & FrancisCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Laser-capture microdissection
mass spectrometry
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proteomics
single-cell proteomics
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References (90)

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