Aconitic acid exporter (aexA) increases organic acid production in Aspergillus

Deng, Shuang; Magnuson, Jon K.; Kim, Joonhoon; Pomraning, Kyle R.; Dai, Ziyu; Hofstad, Beth A.

Aconitic acid exporter (aexA) increases organic acid production in Aspergillus

Patent · Tue Jan 16 04:00:00 EST 2024

OSTI ID:2541684

Deng, Shuang; Magnuson, Jon K.; Kim, Joonhoon; Pomraning, Kyle R.; Dai, Ziyu; Hofstad, Beth A.

Recombinant Aspergillus genetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinant Aspergillus produce more aconitic acid as compared to native Aspergillus. Also provided are methods of using such recombinant Aspergillus to increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

Assignee:: BATTELLE MEMORIAL INSTITUTE (Richland, WA)

Patent Number(s):: 11,873,523

Application Number:: 17/347,109

OSTI ID:: 2541684

Country of Publication:: United States

Language:: English

References (12)

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