CRISPR/Cas9 editing of p-COUMAROYL-CoA:MONOLIGNOL TRANSFERASE 1 in maize alters phenolic metabolism, lignin structure, and lignin-first biomass processing

Oliveira, Dyoni M.; Saleme, Marina L.S.; Smith, Rebecca A.; Vangeel, Thijs; Lima, Marcelo de F.; Chanoca, Alexandra A.; Mota, Thatiane R.; Vanhevel, Yasmine; Coussens, Griet; Van Aelst, Korneel; Geerts, Jordi; Cornet, Iris; Vaneechoutte, Dries; Vandepoele, Klaas; Pauwels, Laurens; Goeminne, Geert; Morreel, Kris; Sels, Bert F.; Ralph, John; Vanholme, Ruben; Boerjan, Wout

doi:10.1016/j.tibtech.2025.01.006

CRISPR/Cas9 editing of p-COUMAROYL-CoA:MONOLIGNOL TRANSFERASE 1 in maize alters phenolic metabolism, lignin structure, and lignin-first biomass processing

Journal Article · Sat Feb 15 00:00:00 EST 2025 · Trends in Biotechnology

DOI:https://doi.org/10.1016/j.tibtech.2025.01.006· OSTI ID:2539881

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Ghent University (Belgium); VIB Center for Plant Systems Biology, Ghent (Belgium)
University of Wisconsin-Madison, WI (United States)
KU Leuven–University of Leuven (Belgium)
University of Antwerp (Belgium)
Ghent University (Belgium); VIB Center for Plant Systems Biology, Ghent (Belgium); VIB Metabolomics Core, Ghent (Belgium)

Valorization of lignocellulosic biomass for sustainable production of high-value chemicals is challenged by the complexity of lignin, a phenolic biopolymer. Beyond the classical lignin monomers derived from p-coumaryl, coniferyl, and sinapyl alcohol, grass lignins incorporate substantial amounts of monolignol p-coumarates that are produced by p-COUMAROYL-CoA:MONOLIGNOL TRANSFERASE (PMT). Here, the CRISPR/Cas9-mediated mutation of ZmPMT1 in maize enabled the design of biomass depleted in p-coumaroylated lignin and enriched in guaiacyl lignin. Lignin-first biorefining of stem biomass from zmpmt1 mutants by reductive catalytic fractionation (RCF) generated a lignin oil depleted in carboxylates and enriched in guaiacyl-derived alcohols, which are desirable substrates for bio-based polyurethane synthesis. Furthermore, the reported lignin engineering in maize is a promising strategy for designing a dual-purpose crop, providing both food and feed, along with a renewable feedstock for the production of plant-based chemicals.

View Accepted Manuscript (DOE)

Research Organization:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018409

OSTI ID:: 2539881

Journal Information:: Trends in Biotechnology, Journal Name: Trends in Biotechnology Journal Issue: 5 Vol. 43; ISSN 0167-7799

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
BAHD acyltransferases
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monolignol conjugates
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CRISPR/Cas9 editing of p-COUMAROYL-CoA:MONOLIGNOL TRANSFERASE 1 in maize alters phenolic metabolism, lignin structure, and lignin-first biomass processing

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