Cell-free synthetic biology for natural product biosynthesis and discovery

Rice, Andrew J.; Sword, Tien T.; Chengan, Kameshwari; Mitchell, Douglas A.; Mouncey, Nigel J.; Moore, Simon J.; Bailey, Constance B.

doi:10.1039/d4cs01198h

Cell-free synthetic biology for natural product biosynthesis and discovery

Journal Article · Wed Mar 19 00:00:00 EDT 2025 · Chemical Society Reviews

DOI:https://doi.org/10.1039/d4cs01198h· OSTI ID:2532452

^[1]; Sword, Tien T. ^[2]; Chengan, Kameshwari ^[3]; ^[1]; ^[4]; ^[5]; Bailey, Constance B. ^[6]

Vanderbilt Univ., Nashville, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States)
University of Kent, Canterbury (United Kingdom)
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)
Imperial College, London (United Kingdom)
Univ. of Sydney, NSW (Australia)

Natural products have applications as biopharmaceuticals, agrochemicals, and other high-value chemicals. However, there are challenges in isolating natural products from their native producers (e.g. bacteria, fungi, plants). In many cases, synthetic chemistry or heterologous expression must be used to access these important molecules. The biosynthetic machinery to generate these compounds is found within biosynthetic gene clusters, primarily consisting of the enzymes that biosynthesise a range of natural product classes (including, but not limited to ribosomal and nonribosomal peptides, polyketides, and terpenoids). Cell-free synthetic biology has emerged in recent years as a bottom-up technology applied towards both prototyping pathways and producing molecules. Recently, it has been applied to natural products, both to characterise biosynthetic pathways and produce new metabolites. This review discusses the core biochemistry of cell-free synthetic biology applied to metabolite production and critiques its advantages and disadvantages compared to whole cell and/or chemical production routes. Specifically, we review the advances in cell-free biosynthesis of ribosomal peptides, analyse the rapid prototyping of natural product biosynthetic enzymes and pathways, highlight advances in novel antimicrobial discovery, and discuss the rising use of cell-free technologies in industrial biotechnology and synthetic biology.

View Journal Article

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2532452

Alternate ID(s):: OSTI ID: 2553917

Journal Information:: Chemical Society Reviews, Journal Name: Chemical Society Reviews Journal Issue: 9 Vol. 54; ISSN 0306-0012

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

References (430)

Use of flow field-flow fractionation for the rapid quantitation of ribosome and ribosomal subunits inEscherichia coli at different protein production conditions Nilsson, Mikael; Bülow, Leif; Wahlund, Karl-Gustav Biotechnology and Bioengineering, Vol. 54, Issue 5 https://doi.org/10.1002/(SICI)1097-0290(19970605)54:5<461::AID-BIT6>3.0.CO;2-C	journal	June 1997
Prolonging cell-free protein synthesis with a novel ATP regeneration system Kim, Dong-Myung; Swartz, James R. Biotechnology and Bioengineering, Vol. 66, Issue 3 https://doi.org/10.1002/(SICI)1097-0290(1999)66:3<180::AID-BIT6>3.0.CO;2-S	journal	January 1999
Phosphite Dehydrogenase: A Versatile Cofactor-Regeneration Enzyme Vrtis, Jennifer M.; White, Andrea K.; Metcalf, William W. Angewandte Chemie International Edition, Vol. 41, Issue 17 https://doi.org/10.1002/1521-3773(20020902)41:17<3257::AID-ANIE3257>3.0.CO;2-N	journal	September 2002
A Cell‐Free Platform Based on Nisin Biosynthesis for Discovering Novel Lanthipeptides and Guiding their Overproduction In Vivo Liu, Ran; Zhang, Yuchen; Zhai, Guoqing Advanced Science, Vol. 7, Issue 17 https://doi.org/10.1002/advs.202001616	journal	July 2020
The Biosynthetic Logic of Polyketide Diversity Hertweck, Christian Angewandte Chemie International Edition, Vol. 48, Issue 26 https://doi.org/10.1002/anie.200806121	journal	June 2009
Functional Analysis of Synthetic Substructures of Polytheonamide B: A Transmembrane Channel‐Forming Peptide Matsuoka, Shigeru; Shinohara, Naoki; Takahashi, Tomoaki Angewandte Chemie International Edition, Vol. 50, Issue 21 https://doi.org/10.1002/anie.201101533	journal	April 2011
Rational Design of Modular Polyketide Synthases: Morphing the Aureothin Pathway into a Luteoreticulin Assembly Line Sugimoto, Yuki; Ding, Ling; Ishida, Keishi Angewandte Chemie International Edition, Vol. 53, Issue 6 https://doi.org/10.1002/anie.201308176	journal	January 2014
An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing α‐Ketone in Polyketide Starter Unit Biosynthesis He, Hai‐Yan; Yuan, Hua; Tang, Man‐Cheng Angewandte Chemie International Edition, Vol. 53, Issue 42 https://doi.org/10.1002/anie.201406602	journal	August 2014
A Dehydratase Domain in Ambruticin Biosynthesis Displays Additional Activity as a Pyran-Forming Cyclase Berkhan, Gesche; Hahn, Frank Angewandte Chemie International Edition, Vol. 53, Issue 51 https://doi.org/10.1002/anie.201407979	journal	October 2014
The Interplay between a Multifunctional Dehydratase Domain and a C-Methyltransferase Effects Olefin Shift in Ambruticin Biosynthesis Berkhan, Gesche; Merten, Christian; Holec, Claudia Angewandte Chemie International Edition, Vol. 55, Issue 43 https://doi.org/10.1002/anie.201607827	journal	September 2016
Biocatalytic Total Synthesis of Ikarugamycin Greunke, Christian; Glöckle, Anna; Antosch, Janine Angewandte Chemie International Edition, Vol. 56, Issue 15 https://doi.org/10.1002/anie.201611063	journal	January 2017
Insights into the Dual Activity of a Bifunctional Dehydratase-Cyclase Domain Sung, Kwang Hoon; Berkhan, Gesche; Hollmann, Tim Angewandte Chemie International Edition, Vol. 57, Issue 1 https://doi.org/10.1002/anie.201707774	journal	December 2017
What is the total number of protein molecules per cell volume? A call to rethink some published values: Insights & Perspectives Milo, Ron BioEssays, Vol. 35, Issue 12 https://doi.org/10.1002/bies.201300066	journal	September 2013
Metabolic costs of amino acid and protein production in Escherichia coli Kaleta, Christoph; Schäuble, Sascha; Rinas, Ursula Biotechnology Journal, Vol. 8, Issue 9 https://doi.org/10.1002/biot.201200267	journal	July 2013
Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool Moore, Simon J.; Lai, Hung-En; Needham, Hannah Biotechnology Journal, Vol. 12, Issue 4 https://doi.org/10.1002/biot.201600678	journal	February 2017
Regeneration of adenosine triphosphate from glycolytic intermediates for cell-free protein synthesis Kim, Dong-Myung; Swartz, James R. Biotechnology and Bioengineering, Vol. 74, Issue 4 https://doi.org/10.1002/bit.1121	journal	January 2001
Mimicking theEscherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis Jewett, Michael C.; Swartz, James R. Biotechnology and Bioengineering, Vol. 86, Issue 1 https://doi.org/10.1002/bit.20026	journal	January 2004
Energizing cell-free protein synthesis with glucose metabolism Calhoun, Kara A.; Swartz, James R. Biotechnology and Bioengineering, Vol. 90, Issue 5 https://doi.org/10.1002/bit.20449	journal	January 2005
Efficient and scalable method for scaling up cell free protein synthesis in batch mode Voloshin, Alexei M.; Swartz, James R. Biotechnology and Bioengineering, Vol. 91, Issue 4 https://doi.org/10.1002/bit.20528	journal	January 2005
Exploiting cell-free systems: Implementation and debugging of a system of biotransformations Bujara, Matthias; Schümperli, Michael; Billerbeck, Sonja Biotechnology and Bioengineering https://doi.org/10.1002/bit.22666	journal	January 2010
Genome-derived minimal metabolic models for Escherichia coli MG1655 with estimated in vivo respiratory ATP stoichiometry Taymaz-Nikerel, Hilal; Borujeni, Amin Espah; Verheijen, Peter J. T. Biotechnology and Bioengineering, Vol. 107, Issue 2 https://doi.org/10.1002/bit.22802	journal	May 2010
Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines Zawada, James F.; Yin, Gang; Steiner, Alexander R. Biotechnology and Bioengineering, Vol. 108, Issue 7 https://doi.org/10.1002/bit.23103	journal	March 2011
Establishing a high yielding streptomyces -based cell-free protein synthesis system: Establishing a Streptomyces -Based CFPS System Li, Jian; Wang, He; Kwon, Yong-Chan Biotechnology and Bioengineering, Vol. 114, Issue 6 https://doi.org/10.1002/bit.26253	journal	February 2017
Increasing cell‐free gene expression yields from linear templates in Escherichia coli and Vibrio natriegens extracts by using DNA‐binding proteins Zhu, Bo; Gan, Rui; Cabezas, Maria D. Biotechnology and Bioengineering, Vol. 117, Issue 12 https://doi.org/10.1002/bit.27538	journal	September 2020
Scaling eukaryotic cell‐free protein synthesis achieved with the versatile and high‐yielding tobacco BY‐2 cell lysate Gupta, Mainak Das; Flaskamp, Yannick; Roentgen, Robin Biotechnology and Bioengineering, Vol. 120, Issue 10 https://doi.org/10.1002/bit.28461	journal	June 2023
Simplifying and streamlining Escherichia coli-based cell-free protein synthesis Yang, William C.; Patel, Kedar G.; Wong, H. Edward Biotechnology Progress, Vol. 28, Issue 2 https://doi.org/10.1002/btpr.1509	journal	February 2012
A simplified and robust protocol for immunoglobulin expression in E scherichia coli cell‐free protein synthesis systems Cai, Qi; Hanson, Jeffrey A.; Steiner, Alexander R. Biotechnology Progress, Vol. 31, Issue 3 https://doi.org/10.1002/btpr.2082	journal	April 2015
Deciphering the Biosynthetic Codes for the Potent Anti-SARS-CoV Cyclodepsipeptide Valinomycin in Streptomyces tsusimaensis ATCC 15141 Cheng, Yi-Qiang ChemBioChem, Vol. 7, Issue 3 https://doi.org/10.1002/cbic.200500425	journal	March 2006
A Polylinker Approach to Reductive Loop Swaps in Modular Polyketide Synthases Kellenberger, Laurenz; Galloway, Ian S.; Sauter, Guido ChemBioChem, Vol. 9, Issue 16 https://doi.org/10.1002/cbic.200800332	journal	November 2008
Cloning and Heterologous Expression of Three Type II PKS Gene Clusters from Streptomyces bottropensis Yan, Xiaohui; Probst, Katharina; Linnenbrink, Anton ChemBioChem, Vol. 13, Issue 2 https://doi.org/10.1002/cbic.201100574	journal	December 2011
Preparative, in Vitro Biocatalysis of Triketide Lactone Chiral Building Blocks Harper, Andrew D.; Bailey, Constance B.; Edwards, Angela D. ChemBioChem, Vol. 13, Issue 15 https://doi.org/10.1002/cbic.201200378	journal	September 2012
Type III Polyketide Synthases: Functional Classification and Phylogenomics Shimizu, Yugo; Ogata, Hiroyuki; Goto, Susumu ChemBioChem, Vol. 18, Issue 1 https://doi.org/10.1002/cbic.201600522	journal	November 2016
Reductive Release from a Hybrid PKS‐NRPS during the Biosynthesis of Pyrichalasin H Heinemann, Henrike; Zhang, Haili; Cox, Russell J. Chemistry – A European Journal, Vol. 30, Issue 4 https://doi.org/10.1002/chem.202302590	journal	November 2023
The Chalcone Synthase Superfamily of Type III Polyketide Synthases Austin, Michael B.; Noel, Joseph P. ChemInform, Vol. 34, Issue 17 https://doi.org/10.1002/chin.200317284	journal	April 2003
An Advanced Apralog with Increased in vitro and in vivo Activity toward Gram‐negative Pathogens and Reduced ex vivo Cochleotoxicity Sonousi, Amr; Quirke, Jonathan C. K.; Waduge, Prabuddha ChemMedChem, Vol. 16, Issue 2 https://doi.org/10.1002/cmdc.202000726	journal	October 2020
Type III polyketide synthases in natural product biosynthesis Yu, Dayu; Xu, Fuchao; Zeng, Jia IUBMB Life, Vol. 64, Issue 4 https://doi.org/10.1002/iub.1005	journal	February 2012
Cloning and characterization of a DNA gyrase B gene from Streptomyces sphaeroides that confers resistance to novobiocin. Thiara, A. S.; Cundliffe, E. The EMBO Journal, Vol. 7, Issue 7 https://doi.org/10.1002/j.1460-2075.1988.tb03065.x	journal	July 1988
Biosynthetic engineering of nonribosomal peptide synthetases: Engineering NRPSs Kries, Hajo Journal of Peptide Science, Vol. 22, Issue 9 https://doi.org/10.1002/psc.2907	journal	July 2016
Characterization of the all‐ E. coli transcription‐translation system myTXTL by mass spectrometry Garenne, David; Beisel, Chase L.; Noireaux, Vincent Rapid Communications in Mass Spectrometry, Vol. 33, Issue 11 https://doi.org/10.1002/rcm.8438	journal	May 2019
A Streptomyces-Based Cell-Free Protein Synthesis System for High-Level Protein Expression Xu, Huiling; Liu, Wan-Qiu; Li, Jian Cell-Free Gene Expression https://doi.org/10.1007/978-1-0716-1998-8_5	book	January 2022
Structural Biology of Nonribosomal Peptide Synthetases Miller, Bradley R.; Gulick, Andrew M. Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-3375-4_1	book	February 2016
Peptide Screening Using PURE Ribosome Display Ohashi, Hiroyuki; Kanamori, Takashi; Osada, Eriko Ribosome Display and Related Technologies https://doi.org/10.1007/978-1-61779-379-0_14	book	October 2011
Coupled transcription — translation in extracts of Streptomyces lividans Thompson, Jill; Rae, Stewart; Cundliffe, Eric Molecular and General Genetics MGG, Vol. 195, Issue 1-2 https://doi.org/10.1007/BF00332721	journal	June 1984
Iterative polyketide biosynthesis by modular polyketide synthases in bacteria Chen, Haotong; Du, Liangcheng Applied Microbiology and Biotechnology, Vol. 100, Issue 2 https://doi.org/10.1007/s00253-015-7093-0	journal	November 2015
The “beauty in the beast”—the multiple uses of Priestia megaterium in biotechnology Biedendieck, Rebekka; Knuuti, Tobias; Moore, Simon J. Applied Microbiology and Biotechnology, Vol. 105, Issue 14-15 https://doi.org/10.1007/s00253-021-11424-6	journal	July 2021
Advances in the adenylation domain: discovery of diverse non-ribosomal peptides Xu, Delei; Zhang, Zihan; Yao, Luye Applied Microbiology and Biotechnology, Vol. 107, Issue 13 https://doi.org/10.1007/s00253-023-12585-2	journal	May 2023
Exploitation of the Streptomyces coelicolor A3(2) genome sequence for discovery of new natural products and biosynthetic pathways Challis, Gregory L. Journal of Industrial Microbiology and Biotechnology, Vol. 41, Issue 2 https://doi.org/10.1007/s10295-013-1383-2	journal	February 2014
Advances in heterologous biosynthesis of plant and fungal natural products by modular co-culture engineering Chen, Tingting; Zhou, Yiyao; Lu, Yinghua Biotechnology Letters, Vol. 41, Issue 1 https://doi.org/10.1007/s10529-018-2619-z	journal	October 2018
Technical Advances to Accelerate Modular Type I Polyketide Synthase Engineering towards a Retro-biosynthetic Platform Pang, Bo; Valencia, Luis E.; Wang, Jessica Biotechnology and Bioprocess Engineering, Vol. 24, Issue 3 https://doi.org/10.1007/s12257-019-0083-9	journal	June 2019
Recent Advances in Re-engineering Modular PKS and NRPS Assembly Lines Beck, Charlotte; Garzón, Jaime Felipe Guerrero; Weber, Tilmann Biotechnology and Bioprocess Engineering, Vol. 25, Issue 6 https://doi.org/10.1007/s12257-020-0265-5	journal	December 2020
Characterization of the cytoplasm of Escherichia coli K-12 as a function of external osmolarity Cayley, Scott; Lewis, Barbara A.; Guttman, Harry J. Journal of Molecular Biology, Vol. 222, Issue 2 https://doi.org/10.1016/0022-2836(91)90212-O	journal	November 1991
Estimation of macromolecule concentrations and excluded volume effects for the cytoplasm of Escherichia coli Zimmerman, Steven B.; Trach, Stefan O. Journal of Molecular Biology, Vol. 222, Issue 3 https://doi.org/10.1016/0022-2836(91)90499-V	journal	December 1991
Biosynthesis of vitamin B12: Timing of the methylation steps between uro'gen III and cobyrinic acid Scott, A. Ian; Mackenzie, Neil E.; Santander, Patricio J. Bioorganic Chemistry, Vol. 12, Issue 4 https://doi.org/10.1016/0045-2068(84)90017-8	journal	December 1984
Generation of polyketide libraries via combinatorial biosynthesis Khosla, Chaitan; Zawada, Robert J. X. Trends in Biotechnology, Vol. 14, Issue 9 https://doi.org/10.1016/0167-7799(96)10046-9	journal	September 1996
Type III Polyketide Synthases in Microorganisms Katsuyama, Yohei; Ohnishi, Yasuo Natural Product Biosynthesis by Microorganisms and Plants, Part A, Vol. 515, p. 359-377 https://doi.org/10.1016/B978-0-12-394290-6.00017-3	book	January 2012
Chapter 2 Analyzing the Decay of Stable RNAs in E. coli Li, Zhongwei; Deutscher, Murray P. Methods in Enzymology https://doi.org/10.1016/S0076-6879(08)02202-7	book	January 2008
Chapter 6 The DEBS Paradigm for Type I Modular Polyketide Synthases and Beyond Katz, Leonard Methods in Enzymology https://doi.org/10.1016/S0076-6879(09)04606-0	book	January 2009
The ubiquitinproteasome pathway is required for processing the NF-κB1 precursor protein and the activation of NF-κB Palombella, Vito J.; Rando, Oliver J.; Goldberg, Alfred L. Cell, Vol. 78, Issue 5 https://doi.org/10.1016/S0092-8674(94)90482-0	journal	September 1994
Mutations in yeast ribosomal proteins S28 and S4 affect the accuracy of translation and alter the sensitivity of the ribosomes to paromomycin Synetos, Dennis; Frantziou, Christina P.; Alksne, Lefa E. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, Vol. 1309, Issue 1-2 https://doi.org/10.1016/S0167-4781(96)00128-5	journal	November 1996
Directed evolution of industrial enzymes Schmidt-Dannert, Claudia; Arnold, Frances H. Trends in Biotechnology, Vol. 17, Issue 4 https://doi.org/10.1016/S0167-7799(98)01283-9	journal	April 1999
Macromolecular crowding: an important but neglected aspect of the intracellular environment Ellis, R. John Current Opinion in Structural Biology, Vol. 11, Issue 1 https://doi.org/10.1016/S0959-440X(00)00172-X	journal	February 2001
Engineering Escherichia coli for the synthesis of taxadiene, a key intermediate in the biosynthesis of taxol Huang, Q. Bioorganic & Medicinal Chemistry, Vol. 9, Issue 9 https://doi.org/10.1016/S0968-0896(01)00072-4	journal	September 2001
Enzymatic synthesis of S-adenosyl-l-methionine on the preparative scale Park, Jeongho; Tai, Junzhe; Roessner, Charles A. Bioorganic & Medicinal Chemistry, Vol. 4, Issue 12 https://doi.org/10.1016/S0968-0896(96)00228-3	journal	December 1996
Knowledge-based design of bimodular and trimodular polyketide synthases based on domain and module swaps: a route to simple statin analogues Ranganathan, Anand; Timoney, Máire; Bycroft, Matthew Chemistry & Biology, Vol. 6, Issue 10 https://doi.org/10.1016/S1074-5521(00)80020-4	journal	October 1999
A hybrid modular polyketide synthase obtained by domain swapping Oliynyk, Markiyan; Brown, Murray J. B.; Cortés, Jesús Chemistry & Biology, Vol. 3, Issue 10 https://doi.org/10.1016/S1074-5521(96)90069-1	journal	October 1996
How corrinoids are synthesized without oxygen: nature's first pathway to vitamin B12 Santander, Patricio J.; Roessner, Charles A.; Stolowich, Neal J. Chemistry & Biology, Vol. 4, Issue 9 https://doi.org/10.1016/S1074-5521(97)90221-0	journal	September 1997
Sequencing and analysis of genes involved in the biosynthesis of a vancomycin group antibiotic van Wageningen, AndréM. A.; Kirkpatrick, Peter N.; Williams, Dudley H. Chemistry & Biology, Vol. 5, Issue 3 https://doi.org/10.1016/S1074-5521(98)90060-6	journal	March 1998
Polyketide biosynthesis beyond the type I, II and III polyketide synthase paradigms Shen, B. Current Opinion in Chemical Biology, Vol. 7, Issue 2 https://doi.org/10.1016/S1367-5931(03)00020-6	journal	April 2003
Mechanism of fluoroquinolone action Drlica, Karl Current Opinion in Microbiology, Vol. 2, Issue 5 https://doi.org/10.1016/S1369-5274(99)00008-9	journal	October 1999
New (and not so new) antibacterial targets – from where and when will the novel drugs come? Projan, S. Current Opinion in Pharmacology, Vol. 2, Issue 5 https://doi.org/10.1016/S1471-4892(02)00197-2	journal	October 2002
Expanding the palette of Streptomyces-based cell-free protein synthesis systems with enhanced yields Li, Jian; Wang, He; Jewett, Michael C. Biochemical Engineering Journal, Vol. 130 https://doi.org/10.1016/j.bej.2017.11.013	journal	February 2018
Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system Caschera, Filippo; Noireaux, Vincent Biochimie, Vol. 99 https://doi.org/10.1016/j.biochi.2013.11.025	journal	April 2014
2-Guanidino-quinazolines as a novel class of translation inhibitors Komarova (Andreyanova), E. S.; Osterman, I. A.; Pletnev, P. I. Biochimie, Vol. 133 https://doi.org/10.1016/j.biochi.2016.11.008	journal	February 2017
A new class of small molecule RNA polymerase inhibitors with activity against Rifampicin-resistant Staphylococcus aureus1 Arhin, Francis; Bélanger, Odette; Ciblat, Stéphane Bioorganic & Medicinal Chemistry, Vol. 14, Issue 17 https://doi.org/10.1016/j.bmc.2006.05.035	journal	September 2006
Widening the bottleneck: Heterologous expression, purification, and characterization of the Ktedonobacter racemifer minimal type II polyketide synthase in Escherichia coli Klein, Joshua G.; Wu, Yang; Kokona, Bashkim Bioorganic & Medicinal Chemistry, Vol. 28, Issue 20 https://doi.org/10.1016/j.bmc.2020.115686	journal	October 2020
The flexizyme system: a highly flexible tRNA aminoacylation tool for the translation apparatus Ohuchi, Masaki; Murakami, Hiroshi; Suga, Hiroaki Current Opinion in Chemical Biology, Vol. 11, Issue 5 https://doi.org/10.1016/j.cbpa.2007.08.011	journal	October 2007
The impact of natural products upon modern drug discovery Ganesan, A. Current Opinion in Chemical Biology, Vol. 12, Issue 3 https://doi.org/10.1016/j.cbpa.2008.03.016	journal	June 2008
Daptomycin: mechanisms of action and resistance, and biosynthetic engineering Baltz, Richard H. Current Opinion in Chemical Biology, Vol. 13, Issue 2 https://doi.org/10.1016/j.cbpa.2009.02.031	journal	April 2009
Biogenesis of the peptide-derived redox cofactor pyrroloquinoline quinone Zhu, Wen; Klinman, Judith P. Current Opinion in Chemical Biology, Vol. 59 https://doi.org/10.1016/j.cbpa.2020.05.001	journal	December 2020
Recent advances in the structural biology of modular polyketide synthases and nonribosomal peptide synthetases Katsuyama, Yohei; Miyanaga, Akimasa Current Opinion in Chemical Biology, Vol. 71 https://doi.org/10.1016/j.cbpa.2022.102223	journal	December 2022
Antimicrobial Resistance in the Intensive Care Unit: Mechanisms, Epidemiology, and Management of Specific Resistant Pathogens Fraimow, Henry S.; Tsigrelis, Constantine Critical Care Clinics, Vol. 27, Issue 1 https://doi.org/10.1016/j.ccc.2010.11.002	journal	January 2011
Exploiting Temporal Collateral Sensitivity in Tumor Clonal Evolution Zhao, Boyang; Sedlak, Joseph C.; Srinivas, Raja Cell, Vol. 165, Issue 1 https://doi.org/10.1016/j.cell.2016.01.045	journal	March 2016
Portable, On-Demand Biomolecular Manufacturing Pardee, Keith; Slomovic, Shimyn; Nguyen, Peter Q. Cell, Vol. 167, Issue 1 https://doi.org/10.1016/j.cell.2016.09.013	journal	September 2016
The Science of Antibiotic Discovery Lewis, Kim Cell, Vol. 181, Issue 1 https://doi.org/10.1016/j.cell.2020.02.056	journal	April 2020
Using a Solid-Phase Ribozyme Aminoacylation System to Reprogram the Genetic Code Murakami, Hiroshi; Kourouklis, Dimitrios; Suga, Hiroaki Chemistry & Biology, Vol. 10, Issue 11 https://doi.org/10.1016/j.chembiol.2003.10.010	journal	November 2003
Genome Mining Reveals a Minimum Gene Set for the Biosynthesis of 32-Membered Macrocyclic Thiopeptides Lactazoles Hayashi, Shohei; Ozaki, Taro; Asamizu, Shumpei Chemistry & Biology, Vol. 21, Issue 5 https://doi.org/10.1016/j.chembiol.2014.03.008	journal	May 2014
One-Pot Synthesis of Azoline-Containing Peptides in a Cell-free Translation System Integrated with a Posttranslational Cyclodehydratase Goto, Yuki; Ito, Yumi; Kato, Yasuharu Chemistry & Biology, Vol. 21, Issue 6 https://doi.org/10.1016/j.chembiol.2014.04.008	journal	June 2014
A Subdomain Swap Strategy for Reengineering Nonribosomal Peptides Kries, Hajo; Niquille, David L.; Hilvert, Donald Chemistry & Biology, Vol. 22, Issue 5 https://doi.org/10.1016/j.chembiol.2015.04.015	journal	May 2015
Comprehensive Structural and Biochemical Analysis of the Terminal Myxalamid Reductase Domain for the Engineered Production of Primary Alcohols Barajas, Jesus F.; Phelan, Ryan M.; Schaub, Andrew J. Chemistry & Biology, Vol. 22, Issue 8 https://doi.org/10.1016/j.chembiol.2015.06.022	journal	August 2015
Introduction to Thiopeptides: Biological Activity, Biosynthesis, and Strategies for Functional Reprogramming Vinogradov, Alexander A.; Suga, Hiroaki Cell Chemical Biology, Vol. 27, Issue 8 https://doi.org/10.1016/j.chembiol.2020.07.003	journal	August 2020
The emerging impact of cell-free chemical biosynthesis Wilding, Kristen M.; Schinn, Song-Min; Long, Emily A. Current Opinion in Biotechnology, Vol. 53 https://doi.org/10.1016/j.copbio.2017.12.019	journal	October 2018
Recent advances in modular co-culture engineering for synthesis of natural products Wang, Rufeng; Zhao, Shujuan; Wang, Zhengtao Current Opinion in Biotechnology, Vol. 62 https://doi.org/10.1016/j.copbio.2019.09.004	journal	April 2020
An analysis of FDA-approved drugs: natural products and their derivatives Patridge, Eric; Gareiss, Peter; Kinch, Michael S. Drug Discovery Today, Vol. 21, Issue 2 https://doi.org/10.1016/j.drudis.2015.01.009	journal	February 2016
Characterization of acetohydroxyacid synthase from Mycobacterium tuberculosis and the identification of its new inhibitor from the screening of a chemical library Choi, Kyoung-Jae; Yu, Yeon Gyu; Hahn, Hoh Gyu FEBS Letters, Vol. 579, Issue 21 https://doi.org/10.1016/j.febslet.2005.07.055	journal	August 2005
Energizing eukaryotic cell-free protein synthesis with glucose metabolism Anderson, Mark J.; Stark, Jessica C.; Hodgman, C. Eric FEBS Letters, Vol. 589, Issue 15 https://doi.org/10.1016/j.febslet.2015.05.045	journal	June 2015
Oxazolidinones: activity, mode of action, and mechanism of resistance Bozdogan, Bülent; Appelbaum, Peter C. International Journal of Antimicrobial Agents, Vol. 23, Issue 2 https://doi.org/10.1016/j.ijantimicag.2003.11.003	journal	February 2004
Computational Tools for Discovering and Engineering Natural Product Biosynthetic Pathways Ren, Hengqian; Shi, Chengyou; Zhao, Huimin iScience, Vol. 23, Issue 1 https://doi.org/10.1016/j.isci.2019.100795	journal	January 2020
The silent pandemic: Emergent antibiotic resistances following the global response to SARS-CoV-2 Mahoney, Andrew R.; Safaee, Mohammad Moein; Wuest, William M. iScience, Vol. 24, Issue 4 https://doi.org/10.1016/j.isci.2021.102304	journal	April 2021
Pyrrhocoricin, a proline-rich antimicrobial peptide derived from insect, inhibits the translation process in the cell-free Escherichia coli protein synthesis system Taniguchi, Masayuki; Ochiai, Akihito; Kondo, Hiroshi Journal of Bioscience and Bioengineering, Vol. 121, Issue 5 https://doi.org/10.1016/j.jbiosc.2015.09.002	journal	May 2016
AmyI-1–18, a cationic α-helical antimicrobial octadecapeptide derived from α-amylase in rice, inhibits the translation and folding processes in a protein synthesis system Taniguchi, Masayuki; Ochiai, Akihito; Fukuda, Shun Journal of Bioscience and Bioengineering, Vol. 122, Issue 4 https://doi.org/10.1016/j.jbiosc.2016.03.004	journal	October 2016
Type II thioesterase improves heterologous biosynthesis of valinomycin in Escherichia coli Li, Jian; Jaitzig, Jennifer; Theuer, Lorenz Journal of Biotechnology, Vol. 193 https://doi.org/10.1016/j.jbiotec.2014.10.037	journal	January 2015
Antibiotics: past, present and future Hutchings, Matthew I.; Truman, Andrew W.; Wilkinson, Barrie Current Opinion in Microbiology, Vol. 51 https://doi.org/10.1016/j.mib.2019.10.008	journal	October 2019
Recent advances in applying cell-free systems for high-value and complex natural product biosynthesis Ji, Xiangyang; Liu, Wan-Qiu; Li, Jian Current Opinion in Microbiology, Vol. 67 https://doi.org/10.1016/j.mib.2022.102142	journal	June 2022
The E. coli S30 lysate proteome: A prototype for cell-free protein production Foshag, Daniel; Henrich, Erik; Hiller, Ekkehard New Biotechnology, Vol. 40 https://doi.org/10.1016/j.nbt.2017.09.005	journal	January 2018
Recombinant cell-lysate-catalysed synthesis of uridine-5′-triphosphate from nucleobase and ribose, and without addition of ATP Loan, Thomas D.; Easton, Christopher J.; Alissandratos, Apostolos New Biotechnology, Vol. 49 https://doi.org/10.1016/j.nbt.2018.10.002	journal	March 2019
Confluence of structural and chemical biology: plant polyketide synthases as biocatalysts for a bio-based future Stewart, Charles; Vickery, Christopher R.; Burkart, Michael D. Current Opinion in Plant Biology, Vol. 16, Issue 3 https://doi.org/10.1016/j.pbi.2013.02.004	journal	June 2013
Bioconversion of recombinantly produced precursor peptide pqqA into pyrroloquinoline quinone (PQQ) using a cell-free in vitro system Wang, Guanglu; Zhou, Yifei; Ma, Ke Protein Expression and Purification, Vol. 178 https://doi.org/10.1016/j.pep.2020.105777	journal	February 2021
Amphotericin B: side effects and toxicity Laniado-Laborín, Rafael; Cabrales-Vargas, Maria Noemí Revista Iberoamericana de Micología, Vol. 26, Issue 4 https://doi.org/10.1016/j.riam.2009.06.003	journal	October 2009
Employing synthetic biology to expand antibiotic discovery Cook, Greta D.; Stasulli, Nikolas M. SLAS Technology, Vol. 29, Issue 2 https://doi.org/10.1016/j.slast.2024.100120	journal	April 2024
Complex peptide natural products: Biosynthetic principles, challenges and opportunities for pathway engineering Wenski, Sebastian L.; Thiengmag, Sirinthra; Helfrich, Eric J. N. Synthetic and Systems Biotechnology, Vol. 7, Issue 1 https://doi.org/10.1016/j.synbio.2022.01.007	journal	March 2022
Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan Tian, Xintong; Liu, Wan-Qiu; Xu, Huiling Synthetic and Systems Biotechnology, Vol. 7, Issue 2 https://doi.org/10.1016/j.synbio.2022.03.006	journal	June 2022
Collateral sensitivity of antibiotic-resistant microbes Pál, Csaba; Papp, Balázs; Lázár, Viktória Trends in Microbiology, Vol. 23, Issue 7 https://doi.org/10.1016/j.tim.2015.02.009	journal	July 2015
A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system Caschera, Filippo; Noireaux, Vincent Metabolic Engineering, Vol. 27 https://doi.org/10.1016/j.ymben.2014.10.007	journal	January 2015
A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery Karim, Ashty S.; Jewett, Michael C. Metabolic Engineering, Vol. 36 https://doi.org/10.1016/j.ymben.2016.03.002	journal	July 2016
Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements Kelwick, Richard; Webb, Alexander J.; MacDonald, James T. Metabolic Engineering, Vol. 38 https://doi.org/10.1016/j.ymben.2016.09.008	journal	November 2016
Multiplexed site-specific genome engineering for overproducing bioactive secondary metabolites in actinomycetes Li, Lei; Zheng, Guosong; Chen, Jun Metabolic Engineering, Vol. 40 https://doi.org/10.1016/j.ymben.2017.01.004	journal	March 2017
Controlling cell-free metabolism through physiochemical perturbations Karim, Ashty S.; Heggestad, Jacob T.; Crowe, Samantha A. Metabolic Engineering, Vol. 45 https://doi.org/10.1016/j.ymben.2017.11.005	journal	January 2018
Generation of a cluster-free Streptomyces albus chassis strains for improved heterologous expression of secondary metabolite clusters Myronovskyi, Maksym; Rosenkränzer, Birgit; Nadmid, Suvd Metabolic Engineering, Vol. 49 https://doi.org/10.1016/j.ymben.2018.09.004	journal	September 2018
Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin Zhuang, Lei; Huang, Shuhui; Liu, Wan-Qiu Metabolic Engineering, Vol. 60 https://doi.org/10.1016/j.ymben.2020.03.009	journal	July 2020
In vitro prototyping of limonene biosynthesis using cell-free protein synthesis Dudley, Quentin M.; Karim, Ashty S.; Nash, Connor J. Metabolic Engineering, Vol. 61 https://doi.org/10.1016/j.ymben.2020.05.006	journal	September 2020
Development of a clostridia-based cell-free system for prototyping genetic parts and metabolic pathways Krüger, Antje; Mueller, Alexander P.; Rybnicky, Grant A. Metabolic Engineering, Vol. 62 https://doi.org/10.1016/j.ymben.2020.06.004	journal	November 2020
A bimodular PKS platform that expands the biological design space Zargar, Amin; Valencia, Luis; Wang, Jessica Metabolic Engineering, Vol. 61 https://doi.org/10.1016/j.ymben.2020.07.001	journal	September 2020
Establishing a versatile toolkit of flux enhanced strains and cell extracts for pathway prototyping Yi, Xiunan; Rasor, Blake J.; Boadi, Nathalie Metabolic Engineering, Vol. 80 https://doi.org/10.1016/j.ymben.2023.10.008	journal	November 2023
Protein synthesis by pure translation systems Shimizu, Yoshihiro; Kanamori, Takashi; Ueda, Takuya Methods, Vol. 36, Issue 3 https://doi.org/10.1016/j.ymeth.2005.04.006	journal	July 2005
Characterization of a novel antibacterial agent that inhibits bacterial translation B??Ddeker, Nina; Bahador, Gina; Gibbs, Craig RNA, Vol. 8, Issue 9 https://doi.org/10.1017/S1355838202024020	journal	September 2002
The RaPID Platform for the Discovery of Pseudo-Natural Macrocyclic Peptides Goto, Yuki; Suga, Hiroaki Accounts of Chemical Research, Vol. 54, Issue 18 https://doi.org/10.1021/acs.accounts.1c00391	journal	September 2021
Lasso Peptides: An Intriguing Class of Bacterial Natural Products Hegemann, Julian D.; Zimmermann, Marcel; Xie, Xiulan Accounts of Chemical Research, Vol. 48, Issue 7 https://doi.org/10.1021/acs.accounts.5b00156	journal	June 2015
Proteomics-Based Tools for Evaluation of Cell-Free Protein Synthesis Hurst, Gregory B.; Asano, Keiji G.; Doktycz, Charles J. Analytical Chemistry, Vol. 89, Issue 21 https://doi.org/10.1021/acs.analchem.7b02555	journal	October 2017
Genetic Code Expansion: A Brief History and Perspective Shandell, Mia A.; Tan, Zhongping; Cornish, Virginia W. Biochemistry, Vol. 60, Issue 46 https://doi.org/10.1021/acs.biochem.1c00286	journal	July 2021
Peptidase Activation by a Leader Peptide-Bound RiPP Recognition Element Kretsch, Ashley M.; Gadgil, Mayuresh G.; DiCaprio, Adam J. Biochemistry, Vol. 62, Issue 4 https://doi.org/10.1021/acs.biochem.2c00700	journal	February 2023
Residue-Specific Peptide Modification: A Chemist’s Guide deGruyter, Justine N.; Malins, Lara R.; Baran, Phil S. Biochemistry, Vol. 56, Issue 30 https://doi.org/10.1021/acs.biochem.7b00536	journal	July 2017
Mechanism of Action of Ribosomally Synthesized and Post-Translationally Modified Peptides Ongpipattanakul, Chayanid; Desormeaux, Emily K.; DiCaprio, Adam Chemical Reviews, Vol. 122, Issue 18 https://doi.org/10.1021/acs.chemrev.2c00210	journal	September 2022
Mechanistic Understanding of Lanthipeptide Biosynthetic Enzymes Repka, Lindsay M.; Chekan, Jonathan R.; Nair, Satish K. Chemical Reviews, Vol. 117, Issue 8 https://doi.org/10.1021/acs.chemrev.6b00591	journal	January 2017
The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines Keatinge-Clay, Adrian T. Chemical Reviews, Vol. 117, Issue 8 https://doi.org/10.1021/acs.chemrev.6b00683	journal	April 2017
Natural Products as Platforms To Overcome Antibiotic Resistance Rossiter, Sean E.; Fletcher, Madison H.; Wuest, William M. Chemical Reviews, Vol. 117, Issue 19 https://doi.org/10.1021/acs.chemrev.7b00283	journal	September 2017
Structure Prediction and Synthesis of Pyridine-Based Macrocyclic Peptide Natural Products Hudson, Graham A.; Hooper, Annie R.; DiCaprio, Adam J. Organic Letters, Vol. 23, Issue 2 https://doi.org/10.1021/acs.orglett.0c02699	journal	August 2020
Engineered Nonribosomal Peptide Synthetase Shows Opposite Amino Acid Loading and Condensation Specificity Stanišić, Aleksa; Hüsken, Annika; Stephan, Philipp ACS Catalysis, Vol. 11, Issue 14 https://doi.org/10.1021/acscatal.1c01270	journal	July 2021
Deep Learning for Prediction and Optimization of Fast-Flow Peptide Synthesis Mohapatra, Somesh; Hartrampf, Nina; Poskus, Mackenzie ACS Central Science, Vol. 6, Issue 12 https://doi.org/10.1021/acscentsci.0c00979	journal	November 2020
Accurate Models of Substrate Preferences of Post-Translational Modification Enzymes from a Combination of mRNA Display and Deep Learning Vinogradov, Alexander A.; Chang, Jun Shi; Onaka, Hiroyasu ACS Central Science, Vol. 8, Issue 6 https://doi.org/10.1021/acscentsci.2c00223	journal	May 2022
Deep Learning-Driven Library Design for the De Novo Discovery of Bioactive Thiopeptides Chang, Jun Shi; Vinogradov, Alexander A.; Zhang, Yue ACS Central Science, Vol. 9, Issue 11 https://doi.org/10.1021/acscentsci.3c00957	journal	November 2023
The Natural Products Atlas: An Open Access Knowledge Base for Microbial Natural Products Discovery van Santen, Jeffrey A.; Jacob, Grégoire; Singh, Amrit Leen ACS Central Science, Vol. 5, Issue 11 https://doi.org/10.1021/acscentsci.9b00806	journal	September 2019
Bioinformatic and Reactivity-Based Discovery of Linaridins Georgiou, Matthew A.; Dommaraju, Shravan R.; Guo, Xiaorui ACS Chemical Biology, Vol. 15, Issue 11 https://doi.org/10.1021/acschembio.0c00620	journal	November 2020
Substrate Recognition by the Peptidyl-(S)-2-mercaptoglycine Synthase TglHI during 3-Thiaglutamate Biosynthesis McLaughlin, Martin I.; Yu, Yue; van der Donk, Wilfred A. ACS Chemical Biology, Vol. 17, Issue 4 https://doi.org/10.1021/acschembio.2c00087	journal	April 2022
Heterologous Gene Expression of N -Terminally Truncated Variants of LipPks1 Suggests a Functionally Critical Structural Motif in the N -terminus of Modular Polyketide Synthase Yuzawa, Satoshi; Bailey, Constance B.; Fujii, Tatsuya ACS Chemical Biology, Vol. 12, Issue 11 https://doi.org/10.1021/acschembio.7b00714	journal	October 2017
Structural and Functional Studies of a Pyran Synthase Domain from a trans-Acyltransferase Assembly Line Wagner, Drew T.; Zhang, Zhicheng; Meoded, Roy A. ACS Chemical Biology, Vol. 13, Issue 4 https://doi.org/10.1021/acschembio.8b00049	journal	February 2018
Bioactivity-HiTES Unveils Cryptic Antibiotics Encoded in Actinomycete Bacteria Moon, Kyuho; Xu, Fei; Zhang, Chen ACS Chemical Biology, Vol. 14, Issue 4 https://doi.org/10.1021/acschembio.9b00049	journal	March 2019
Heterologous Biosynthesis of Type II Polyketide Products Using E. coli Liu, Xiangyang; Hua, Kangmin; Liu, Dongxu ACS Chemical Biology, Vol. 15, Issue 5 https://doi.org/10.1021/acschembio.9b00827	journal	December 2019
Multicomponent Microscale Biosynthesis of Unnatural Cyanobacterial Indole Alkaloids Khatri, Yogan; Hohlman, Robert M.; Mendoza, Johnny ACS Synthetic Biology, Vol. 9, Issue 6 https://doi.org/10.1021/acssynbio.0c00038	journal	April 2020
Translation Related Factors Improve the Productivity of a Streptomyces -Based Cell-Free Protein Synthesis System Xu, Huiling; Liu, Wan-Qiu; Li, Jian ACS Synthetic Biology, Vol. 9, Issue 5 https://doi.org/10.1021/acssynbio.0c00140	journal	April 2020
Cell-Free Synthesis of Natural Compounds from Genomic DNA of Biosynthetic Gene Clusters Siebels, Ilka; Nowak, Sarah; Heil, Christina S. ACS Synthetic Biology, Vol. 9, Issue 9 https://doi.org/10.1021/acssynbio.0c00186	journal	August 2020
A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology Moore, Simon J.; Lai, Hung-En; Chee, Soo-Mei ACS Synthetic Biology, Vol. 10, Issue 2 https://doi.org/10.1021/acssynbio.0c00581	journal	January 2021
Tuning Cell-Free Composition Controls the Time Delay, Dynamics, and Productivity of TX-TL Expression Vezeau, Grace E.; Salis, Howard M. ACS Synthetic Biology, Vol. 10, Issue 10 https://doi.org/10.1021/acssynbio.1c00136	journal	September 2021
Improving the Intensity of Integrated Expression for Microbial Production Wang, Zi-Kai; Gong, Jin-Song; Qin, Jiufu ACS Synthetic Biology, Vol. 10, Issue 11 https://doi.org/10.1021/acssynbio.1c00334	journal	November 2021
Regulatory Part Engineering for High-Yield Protein Synthesis in an All-Streptomyces-Based Cell-Free Expression System Xu, Huiling; Yang, Chen; Tian, Xintong ACS Synthetic Biology, Vol. 11, Issue 2, p. 570-578 https://doi.org/10.1021/acssynbio.1c00587	journal	February 2022
Engineered Production of Strictosidine and Analogues in Yeast Misa, Joshua; Billingsley, John M.; Niwa, Kanji ACS Synthetic Biology, Vol. 11, Issue 4 https://doi.org/10.1021/acssynbio.2c00037	journal	March 2022
Cell-Free Mutant Analysis Combined with Structure Prediction of a Lasso Peptide Biosynthetic Protease B2 Alfi, Almasul; Popov, Aleksandr; Kumar, Ashutosh ACS Synthetic Biology, Vol. 11, Issue 6 https://doi.org/10.1021/acssynbio.2c00176	journal	June 2022
Mechanistic Insights into Cell-Free Gene Expression through an Integrated -Omics Analysis of Extract Processing Methods Rasor, Blake J.; Chirania, Payal; Rybnicky, Grant A. ACS Synthetic Biology, Vol. 12, Issue 2 https://doi.org/10.1021/acssynbio.2c00339	journal	January 2023
Investigating and Optimizing the Lysate-Based Expression of Nonribosomal Peptide Synthetases Using a Reporter System Dinglasan, Jaime Lorenzo N.; Sword, Tien T.; Barker, J. William ACS Synthetic Biology, Vol. 12, Issue 5 https://doi.org/10.1021/acssynbio.2c00658	journal	April 2023
One-Pot De Novo Synthesis of [4Fe-4S] Proteins Using a Recombinant SUF System under Aerobic Conditions Wang, Po-Hsiang; Nishikawa, Shota; McGlynn, Shawn Erin ACS Synthetic Biology, Vol. 12, Issue 10 https://doi.org/10.1021/acssynbio.3c00155	journal	July 2023
Module-Based Polyketide Synthase Engineering for de Novo Polyketide Biosynthesis Nava, Alberto A.; Roberts, Jacob; Haushalter, Robert W. ACS Synthetic Biology, Vol. 12, Issue 11 https://doi.org/10.1021/acssynbio.3c00282	journal	October 2023
Engineering a Polyketide Synthase for In Vitro Production of Adipic Acid Hagen, Andrew; Poust, Sean; Rond, Tristan de ACS Synthetic Biology, Vol. 5, Issue 1 https://doi.org/10.1021/acssynbio.5b00153	journal	November 2015
The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology Garamella, Jonathan; Marshall, Ryan; Rustad, Mark ACS Synthetic Biology, Vol. 5, Issue 4 https://doi.org/10.1021/acssynbio.5b00296	journal	February 2016
EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology Moore, Simon J.; Lai, Hung-En; Kelwick, Richard J. R. ACS Synthetic Biology, Vol. 5, Issue 10 https://doi.org/10.1021/acssynbio.6b00031	journal	May 2016
Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis Dudley, Quentin M.; Anderson, Kim C.; Jewett, Michael C. ACS Synthetic Biology, Vol. 5, Issue 12 https://doi.org/10.1021/acssynbio.6b00154	journal	July 2016
In Vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis Goering, Anthony W.; Li, Jian; McClure, Ryan A. ACS Synthetic Biology, Vol. 6, Issue 1 https://doi.org/10.1021/acssynbio.6b00160	journal	August 2016
Sequence Specific Modeling of E. coli Cell-Free Protein Synthesis Vilkhovoy, Michael; Horvath, Nicholas; Shih, Che-Hsiao ACS Synthetic Biology, Vol. 7, Issue 8 https://doi.org/10.1021/acssynbio.7b00465	journal	June 2018
Establishing a Cell-Free Vibrio natriegens Expression System Wiegand, Daniel J.; Lee, Henry H.; Ostrov, Nili ACS Synthetic Biology, Vol. 7, Issue 10 https://doi.org/10.1021/acssynbio.8b00222	journal	August 2018
Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens Des Soye, Benjamin J.; Davidson, Samuel R.; Weinstock, Matthew T. ACS Synthetic Biology, Vol. 7, Issue 9 https://doi.org/10.1021/acssynbio.8b00252	journal	August 2018
A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System Lavickova, Barbora; Maerkl, Sebastian J. ACS Synthetic Biology, Vol. 8, Issue 2 https://doi.org/10.1021/acssynbio.8b00427	journal	January 2019
PERSIA for Direct Fluorescence Measurements of Transcription, Translation, and Enzyme Activity in Cell-Free Systems Wick, Scott; Walsh, David I.; Bobrow, Johanna ACS Synthetic Biology, Vol. 8, Issue 5 https://doi.org/10.1021/acssynbio.8b00450	journal	March 2019
In Vitro Selection of Peptides and Proteins—Advantages of mRNA Display Newton, Matilda S.; Cabezas-Perusse, Yari; Tong, Cher Ling ACS Synthetic Biology, Vol. 9, Issue 2 https://doi.org/10.1021/acssynbio.9b00419	journal	December 2019
A Bifunctional Polyphosphate Kinase Driving the Regeneration of Nucleoside Triphosphate and Reconstituted Cell-Free Protein Synthesis Wang, Po-Hsiang; Fujishima, Kosuke; Berhanu, Samuel ACS Synthetic Biology, Vol. 9, Issue 1 https://doi.org/10.1021/acssynbio.9b00456	journal	December 2019
Novel Tetrapeptide Inhibitors of Bacterial Protein Synthesis Produced by a Streptomyces sp. Brandi, Letizia; Lazzarini, Ameriga; Cavaletti, Linda Biochemistry, Vol. 45, Issue 11 https://doi.org/10.1021/bi052540k	journal	February 2006
Functional Modular Dissection of DEBS1-TE Changes Triketide Lactone Ratios and Provides Insight into Acyl Group Loading, Hydrolysis, and ACP Transfer Yan, John; Hazzard, Christopher; Bonnett, Shilah A. Biochemistry, Vol. 51, Issue 46 https://doi.org/10.1021/bi300830q	journal	November 2012
Broad Substrate Specificity of the Loading Didomain of the Lipomycin Polyketide Synthase Yuzawa, Satoshi; Eng, Clara H.; Katz, Leonard Biochemistry, Vol. 52, Issue 22 https://doi.org/10.1021/bi400520t	journal	May 2013
Orthoformimycin, a Selective Inhibitor of Bacterial Translation Elongation fromStreptomycesContaining an Unusual Orthoformate Maffioli, Sonia I.; Fabbretti, Attilio; Brandi, Letizia ACS Chemical Biology, Vol. 8, Issue 9 https://doi.org/10.1021/cb4004095	journal	August 2013
A Double-Hotdog with a New Trick: Structure and Mechanism of the trans -Acyltransferase Polyketide Synthase Enoyl-isomerase Gay, Darren C.; Spear, Philip J.; Keatinge-Clay, Adrian T. ACS Chemical Biology, Vol. 9, Issue 10 https://doi.org/10.1021/cb500459b	journal	August 2014
HIV Protease Inhibitors Block Streptolysin S Production Maxson, Tucker; Deane, Caitlin D.; Molloy, Evelyn M. ACS Chemical Biology, Vol. 10, Issue 5 https://doi.org/10.1021/cb500843r	journal	February 2015
Extended-Connectivity Fingerprints Rogers, David; Hahn, Mathew Journal of Chemical Information and Modeling, Vol. 50, Issue 5 https://doi.org/10.1021/ci100050t	journal	April 2010
Thiopeptide Antibiotics Bagley, Mark C.; Dale, James W.; Merritt, Eleanor A. Chemical Reviews, Vol. 105, Issue 2 https://doi.org/10.1021/cr0300441	journal	February 2005
Biosynthesis of the Vancomycin Group of Antibiotics: Involvement of an Unusual Dioxygenase in the Pathway to ( S )-4-Hydroxyphenylglycine Choroba, Oliver W.; Williams, Dudley H.; Spencer, Jonathan B. Journal of the American Chemical Society, Vol. 122, Issue 22 https://doi.org/10.1021/ja000076v	journal	June 2000
Polytheonamides A and B, Highly Cytotoxic, Linear Polypeptides with Unprecedented Structural Features, from the Marine Sponge, Theonella s winhoei Hamada, Toshiyuki; Matsunaga, Shigeki; Yano, Gen Journal of the American Chemical Society, Vol. 127, Issue 1 https://doi.org/10.1021/ja045749e	journal	December 2004
Characterization of the Cereulide NRPS α-Hydroxy Acid Specifying Modules: Activation of α-Keto Acids and Chiral Reduction on the Assembly Line Magarvey, Nathan A.; Ehling-Schulz, Monika; Walsh, Christopher T. Journal of the American Chemical Society, Vol. 128, Issue 33 https://doi.org/10.1021/ja0640187	journal	August 2006
Ribosomal Synthesis of an Amphotericin-B Inspired Macrocycle Torikai, Kohei; Suga, Hiroaki Journal of the American Chemical Society, Vol. 136, Issue 50 https://doi.org/10.1021/ja508648s	journal	December 2014
Identifying the Minimal Enzymes Required for Anhydrotetracycline Biosynthesis Zhang, Wenjun; Watanabe, Kenji; Cai, Xiaolu Journal of the American Chemical Society, Vol. 130, Issue 19 https://doi.org/10.1021/ja800951e	journal	April 2008
Ribosomal Synthesis of Polypeptoids and Peptoid−Peptide Hybrids Kawakami, Takashi; Murakami, Hiroshi; Suga, Hiroaki Journal of the American Chemical Society, Vol. 130, Issue 50 https://doi.org/10.1021/ja806998v	journal	November 2008
Complete Reconstitution and Deorphanization of the 3 MDa Nocardiosis-Associated Polyketide Synthase Yuet, Kai P.; Liu, Corey W.; Lynch, Stephen R. Journal of the American Chemical Society, Vol. 142, Issue 13 https://doi.org/10.1021/jacs.0c00904	journal	March 2020
Exploring the Post-translational Enzymology of PaaA by mRNA Display Fleming, Steven R.; Himes, Paul M.; Ghodge, Swapnil V. Journal of the American Chemical Society, Vol. 142, Issue 11 https://doi.org/10.1021/jacs.0c01576	journal	February 2020
Chemoinformatic-Guided Engineering of Polyketide Synthases Zargar, Amin; Lal, Ravi; Valencia, Luis Journal of the American Chemical Society, Vol. 142, Issue 22 https://doi.org/10.1021/jacs.0c02549	journal	May 2020
Promiscuous Enzymes Cooperate at the Substrate Level En Route to Lactazole A Vinogradov, Alexander A.; Shimomura, Morito; Kano, Naokazu Journal of the American Chemical Society, Vol. 142, Issue 32 https://doi.org/10.1021/jacs.0c05541	journal	July 2020
Accurate Broadcasting of Substrate Fitness for Lactazole Biosynthetic Pathway from Reactivity-Profiling mRNA Display Vinogradov, Alexander A.; Nagai, Emiko; Chang, Jun Shi Journal of the American Chemical Society, Vol. 142, Issue 48 https://doi.org/10.1021/jacs.0c10374	journal	November 2020
Modular Chemoenzymatic Synthesis of GE81112 B1 and Related Analogues Enables Elucidation of Its Key Pharmacophores Zwick, Christian R.; Sosa, Max B.; Renata, Hans Journal of the American Chemical Society, Vol. 143, Issue 3 https://doi.org/10.1021/jacs.0c13424	journal	January 2021
Cell-Free Biosynthesis to Evaluate Lasso Peptide Formation and Enzyme–Substrate Tolerance Si, Yuanyuan; Kretsch, Ashley M.; Daigh, Laura M. Journal of the American Chemical Society, Vol. 143, Issue 15 https://doi.org/10.1021/jacs.1c01452	journal	April 2021
Site-Specific Nonenzymatic Peptide S/O-Glutamylation Reveals the Extent of Substrate Promiscuity in Glutamate Elimination Domains Vinogradov, Alexander A.; Nagano, Masanobu; Goto, Yuki Journal of the American Chemical Society, Vol. 143, Issue 33 https://doi.org/10.1021/jacs.1c06470	journal	August 2021
Accessing Diverse Pyridine-Based Macrocyclic Peptides by a Two-Site Recognition Pathway Nguyen, Dinh T.; Le, Tung T.; Rice, Andrew J. Journal of the American Chemical Society, Vol. 144, Issue 25 https://doi.org/10.1021/jacs.2c02824	journal	June 2022
Enzymatic Pyridine Aromatization during Thiopeptide Biosynthesis Rice, Andrew J.; Pelton, Jarrett M.; Kramer, Nicholas J. Journal of the American Chemical Society, Vol. 144, Issue 46 https://doi.org/10.1021/jacs.2c07377	journal	November 2022
De Novo Discovery of Thiopeptide Pseudo-natural Products Acting as Potent and Selective TNIK Kinase Inhibitors Vinogradov, Alexander A.; Zhang, Yue; Hamada, Keisuke Journal of the American Chemical Society, Vol. 144, Issue 44 https://doi.org/10.1021/jacs.2c07937	journal	October 2022
Lasso Peptides: Exploring the Folding Landscape of Nature’s Smallest Interlocked Motifs da Hora, Gabriel C. A.; Oh, Myongin; Mifflin, Marcus C. Journal of the American Chemical Society, Vol. 146, Issue 7 https://doi.org/10.1021/jacs.3c10126	journal	January 2024
Cheminformatics-Guided Cell-Free Exploration of Peptide Natural Products Pelton, Jarrett M.; Hochuli, Joshua E.; Sadecki, Patric W. Journal of the American Chemical Society, Vol. 146, Issue 12 https://doi.org/10.1021/jacs.3c11306	journal	March 2024
A Compact Reprogrammed Genetic Code for De Novo Discovery of Proteolytically Stable Thiopeptides Vinogradov, Alexander A.; Zhang, Yue; Hamada, Keisuke Journal of the American Chemical Society, Vol. 146, Issue 12 https://doi.org/10.1021/jacs.3c12037	journal	March 2024
Chemoenzymatic Synthesis of Thiazolyl Peptide Natural Products Featuring an Enzyme-Catalyzed Formal [4 + 2] Cycloaddition Wever, Walter J.; Bogart, Jonathan W.; Baccile, Joshua A. Journal of the American Chemical Society, Vol. 137, Issue 10 https://doi.org/10.1021/jacs.5b00940	journal	March 2015
In Vitro Biosynthesis of the Core Scaffold of the Thiopeptide Thiomuracin Hudson, Graham A.; Zhang, Zhengan; Tietz, Jonathan I. Journal of the American Chemical Society, Vol. 137, Issue 51 https://doi.org/10.1021/jacs.5b10194	journal	December 2015
Biosynthetic Timing and Substrate Specificity for the Thiopeptide Thiomuracin Zhang, Zhengan; Hudson, Graham A.; Mahanta, Nilkamal Journal of the American Chemical Society, Vol. 138, Issue 48 https://doi.org/10.1021/jacs.6b08987	journal	October 2016
Discovery of a Cryptic Antifungal Compound from Streptomyces albus J1074 Using High-Throughput Elicitor Screens Xu, Fei; Nazari, Behnam; Moon, Kyuho Journal of the American Chemical Society, Vol. 139, Issue 27 https://doi.org/10.1021/jacs.7b02716	journal	June 2017
Ribosomal Synthesis of Backbone-Cyclic Peptides Compatible with In Vitro Display Takatsuji, Ryo; Shinbara, Koki; Katoh, Takayuki Journal of the American Chemical Society, Vol. 141, Issue 6 https://doi.org/10.1021/jacs.8b05327	journal	January 2019
Solid-Phase Total Synthesis and Dual Mechanism of Action of the Channel-Forming 48-mer Peptide Polytheonamide B Hayata, Atsushi; Itoh, Hiroaki; Inoue, Masayuki Journal of the American Chemical Society, Vol. 140, Issue 33 https://doi.org/10.1021/jacs.8b06755	journal	July 2018
Total Enzyme Syntheses of Napyradiomycins A1 and B1 McKinnie, Shaun M. K.; Miles, Zachary D.; Jordan, Peter A. Journal of the American Chemical Society, Vol. 140, Issue 51 https://doi.org/10.1021/jacs.8b10134	journal	December 2018
Flexizyme-Enabled Benchtop Biosynthesis of Thiopeptides Fleming, Steven R.; Bartges, Tessa E.; Vinogradov, Alexander A. Journal of the American Chemical Society, Vol. 141, Issue 2 https://doi.org/10.1021/jacs.8b11521	journal	January 2019
Structure−Activity Relationships of Polymyxin Antibiotics Velkov, Tony; Thompson, Philip E.; Nation, Roger L. Journal of Medicinal Chemistry, Vol. 53, Issue 5 https://doi.org/10.1021/jm900999h	journal	October 2009
New Substructure Filters for Removal of Pan Assay Interference Compounds (PAINS) from Screening Libraries and for Their Exclusion in Bioassays Baell, Jonathan B.; Holloway, Georgina A. Journal of Medicinal Chemistry, Vol. 53, Issue 7 https://doi.org/10.1021/jm901137j	journal	April 2010
Nonribosomal Peptide Synthetases Involved in the Production of Medically Relevant Natural Products Felnagle, Elizabeth A.; Jackson, Emily E.; Chan, Yolande A. Molecular Pharmaceutics, Vol. 5, Issue 2 https://doi.org/10.1021/mp700137g	journal	January 2008
Cloning and Characterization of the Bleomycin Biosynthetic Gene Cluster from Streptomyces verticillus ATCC15003 Shen, Ben; Du, Liangcheng; Sanchez, Cesar Journal of Natural Products, Vol. 65, Issue 3 https://doi.org/10.1021/np010550q	journal	February 2002
Structural Characterization of Daptomycin Analogues A21978C_1-3(d-Asn₁₁) Produced by a Recombinant Streptomyces roseosporus Strain Gu, Jian-Qiao; Nguyen, Kien T.; Gandhi, Chhayal Journal of Natural Products, Vol. 70, Issue 2 https://doi.org/10.1021/np0605135	journal	February 2007
In Vitro Biosynthesis of Unnatural Enterocin and Wailupemycin Polyketides Kalaitzis, John A.; Cheng, Qian; Thomas, Paul M. Journal of Natural Products, Vol. 72, Issue 3 https://doi.org/10.1021/np800598t	journal	February 2009
Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System Sun, Zachary Z.; Yeung, Enoch; Hayes, Clarmyra A. ACS Synthetic Biology, Vol. 3, Issue 6 https://doi.org/10.1021/sb400131a	journal	December 2013
Gene Circuit Performance Characterization and Resource Usage in a Cell-Free “Breadboard” Siegal-Gaskins, Dan; Tuza, Zoltan A.; Kim, Jongmin ACS Synthetic Biology, Vol. 3, Issue 6 https://doi.org/10.1021/sb400203p	journal	April 2014
Rapidly Characterizing the Fast Dynamics of RNA Genetic Circuitry with Cell-Free Transcription–Translation (TX-TL) Systems Takahashi, Melissa K.; Chappell, James; Hayes, Clarmyra A. ACS Synthetic Biology, Vol. 4, Issue 5 https://doi.org/10.1021/sb400206c	journal	March 2014
High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System Cobb, Ryan E.; Wang, Yajie; Zhao, Huimin ACS Synthetic Biology, Vol. 4, Issue 6 https://doi.org/10.1021/sb500351f	journal	November 2014
Engineering biodiversity with type II polyketide synthase genes Moore, Bradley S.; Piel, Jörn Antonie van Leeuwenhoek, Vol. 78, Issue 3/4, p. 391-398 https://doi.org/10.1023/A:1010273224820	journal	December 2000
Aspects of the biosynthesis of non-aromatic fungal polyketides by iterative polyketide synthases Hutchinson, C. Richard; Kennedy, Jonathan; Park, Cheonseok Antonie van Leeuwenhoek, Vol. 78, Issue 3/4, p. 287-295 https://doi.org/10.1023/A:1010294330190	journal	December 2000
Cell-free synthesis of polyketides by recombinant erythromycin polyketide synthases Pieper, Rembert; Luo, Guanglin; Cane, David E. Nature, Vol. 378, Issue 6554 https://doi.org/10.1038/378263a0	journal	November 1995
Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2) Bentley, S. D.; Chater, K. F.; Cerdeño-Tárraga, A. -M. Nature, Vol. 417, Issue 6885 https://doi.org/10.1038/417141a	journal	May 2002
Living factories of the future Eisenstein, Michael Nature, Vol. 531, Issue 7594 https://doi.org/10.1038/531401a	journal	March 2016
Cell-free translation reconstituted with purified components Shimizu, Yoshihiro; Inoue, Akio; Tomari, Yukihide Nature Biotechnology, Vol. 19, Issue 8 https://doi.org/10.1038/90802	journal	August 2001
Insights into polyketide biosynthesis gained from repurposing antibiotic-producing polyketide synthases to produce fuels and chemicals Yuzawa, Satoshi; Keasling, Jay D.; Katz, Leonard The Journal of Antibiotics, Vol. 69, Issue 7 https://doi.org/10.1038/ja.2016.64	journal	June 2016
An integrated cell-free metabolic platform for protein production and synthetic biology Jewett, Michael C.; Calhoun, Kara A.; Voloshin, Alexei Molecular Systems Biology, Vol. 4, Issue 1, Article No. 220 https://doi.org/10.1038/msb.2008.57	journal	October 2008
High-level semi-synthetic production of the potent antimalarial artemisinin Paddon, C. J.; Westfall, P. J.; Pitera, D. J. Nature, Vol. 496, Issue 7446 https://doi.org/10.1038/nature12051	journal	April 2013
Structure and mechanism of the tRNA-dependent lantibiotic dehydratase NisB Ortega, Manuel A.; Hao, Yue; Zhang, Qi Nature, Vol. 517, Issue 7535 https://doi.org/10.1038/nature13888	journal	October 2014
Predictive compound accumulation rules yield a broad-spectrum antibiotic Richter, Michelle F.; Drown, Bryon S.; Riley, Andrew P. Nature, Vol. 545, Issue 7654 https://doi.org/10.1038/nature22308	journal	May 2017
Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli Bennett, Bryson D.; Kimball, Elizabeth H.; Gao, Melissa Nature Chemical Biology, Vol. 5, Issue 8 https://doi.org/10.1038/nchembio.186	journal	June 2009
The structural biology of biosynthetic megaenzymes Weissman, Kira J. Nature Chemical Biology, Vol. 11, Issue 9 https://doi.org/10.1038/nchembio.1883	journal	August 2015
Enzymatic total synthesis of enterocin polyketides Cheng, Qian; Xiang, Longkuan; Izumikawa, Miho Nature Chemical Biology, Vol. 3, Issue 9 https://doi.org/10.1038/nchembio.2007.22	journal	August 2007
YcaO domains use ATP to activate amide backbones during peptide cyclodehydrations Dunbar, Kyle L.; Melby, Joel O.; Mitchell, Douglas A. Nature Chemical Biology, Vol. 8, Issue 6 https://doi.org/10.1038/nchembio.944	journal	April 2012
Structural and mechanistic insights into polyketide macrolactonization from polyketide-based affinity labels Giraldes, John W.; Akey, David L.; Kittendorf, Jeffrey D. Nature Chemical Biology, Vol. 2, Issue 10 https://doi.org/10.1038/nchembio822	journal	September 2006
Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo Ozaki, Taro; Yamashita, Kona; Goto, Yuki Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms14207	journal	February 2017
A synthetic biochemistry platform for cell free production of monoterpenes from glucose Korman, Tyler P.; Opgenorth, Paul H.; Bowie, James U. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms15526	journal	May 2017
Quantifying cellular capacity identifies gene expression designs with reduced burden Ceroni, Francesca; Algar, Rhys; Stan, Guy-Bart Nature Methods, Vol. 12, Issue 5 https://doi.org/10.1038/nmeth.3339	journal	April 2015
Vibrio natriegens as a fast-growing host for molecular biology Weinstock, Matthew T.; Hesek, Eric D.; Wilson, Christopher M. Nature Methods, Vol. 13, Issue 10 https://doi.org/10.1038/nmeth.3970	journal	August 2016
A highly flexible tRNA acylation method for non-natural polypeptide synthesis Murakami, Hiroshi; Ohta, Atsushi; Ashigai, Hiroshi Nature Methods, Vol. 3, Issue 5 https://doi.org/10.1038/nmeth877	journal	April 2006
Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes Reetz, Manfred T.; Carballeira, José Daniel Nature Protocols, Vol. 2, Issue 4 https://doi.org/10.1038/nprot.2007.72	journal	April 2007
Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach Bennett, Bryson D.; Yuan, Jie; Kimball, Elizabeth H. Nature Protocols, Vol. 3, Issue 8 https://doi.org/10.1038/nprot.2008.107	journal	July 2008
Flexizymes for genetic code reprogramming Goto, Yuki; Katoh, Takayuki; Suga, Hiroaki Nature Protocols, Vol. 6, Issue 6 https://doi.org/10.1038/nprot.2011.331	journal	May 2011
Drugs for bad bugs: confronting the challenges of antibacterial discovery Payne, David J.; Gwynn, Michael N.; Holmes, David J. Nature Reviews Drug Discovery, Vol. 6, Issue 1 https://doi.org/10.1038/nrd2201	journal	December 2006
Platforms for antibiotic discovery Lewis, Kim Nature Reviews Drug Discovery, Vol. 12, Issue 5 https://doi.org/10.1038/nrd3975	journal	April 2013
ESKAPEing the labyrinth of antibacterial discovery Tommasi, Ruben; Brown, Dean G.; Walkup, Grant K. Nature Reviews Drug Discovery, Vol. 14, Issue 8 https://doi.org/10.1038/nrd4572	journal	July 2015
The antibiotic resistome: the nexus of chemical and genetic diversity Wright, Gerard D. Nature Reviews Microbiology, Vol. 5, Issue 3 https://doi.org/10.1038/nrmicro1614	journal	March 2007
New insights into the formation of fungal aromatic polyketides Crawford, Jason M.; Townsend, Craig A. Nature Reviews Microbiology, Vol. 8, Issue 12 https://doi.org/10.1038/nrmicro2465	journal	November 2010
Streptolysin S-like virulence factors: the continuing sagA Molloy, Evelyn M.; Cotter, Paul D.; Hill, Colin Nature Reviews Microbiology, Vol. 9, Issue 9 https://doi.org/10.1038/nrmicro2624	journal	August 2011
Molecular mechanisms of antibiotic resistance Blair, Jessica M. A.; Webber, Mark A.; Baylay, Alison J. Nature Reviews Microbiology, Vol. 13, Issue 1 https://doi.org/10.1038/nrmicro3380	journal	December 2014
The antibiotic kasugamycin mimics mRNA nucleotides to destabilize tRNA binding and inhibit canonical translation initiation Schluenzen, Frank; Takemoto, Chie; Wilson, Daniel N. Nature Structural & Molecular Biology, Vol. 13, Issue 10 https://doi.org/10.1038/nsmb1145	journal	September 2006
Short-chain ketone production by engineered polyketide synthases in Streptomyces albus Yuzawa, Satoshi; Mirsiaghi, Mona; Jocic, Renee Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-07040-0	journal	November 2018
Expanding the limits of the second genetic code with ribozymes Lee, Joongoo; Schwieter, Kenneth E.; Watkins, Andrew M. Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-12916-w	journal	November 2019
An in vitro platform for engineering and harnessing modular polyketide synthases Miyazawa, Takeshi; Hirsch, Melissa; Zhang, Zhicheng Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-019-13811-0	journal	January 2020
U1 snRNP regulates cancer cell migration and invasion in vitro Oh, Jung-Min; Venters, Christopher C.; Di, Chao Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-019-13993-7	journal	January 2020
In vitro ribosome synthesis and evolution through ribosome display Hammerling, Michael J.; Fritz, Brian R.; Yoesep, Danielle J. Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-14705-2	journal	February 2020
Large scale active-learning-guided exploration for in vitro protein production optimization Borkowski, Olivier; Koch, Mathilde; Zettor, Agnès Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-15798-5	journal	April 2020
Minimal lactazole scaffold for in vitro thiopeptide bioengineering Vinogradov, Alexander A.; Shimomura, Morito; Goto, Yuki Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-16145-4	journal	May 2020
Animal biosynthesis of complex polyketides in a photosynthetic partnership Torres, Joshua P.; Lin, Zhenjian; Winter, Jaclyn M. Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-16376-5	journal	June 2020
Toxin import through the antibiotic efflux channel TolC Housden, Nicholas G.; Webby, Melissa N.; Lowe, Edward D. Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-24930-y	journal	July 2021
Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform Zhang, Linyue; King, Edward; Black, William B. Nature Communications, Vol. 13, Issue 1 https://doi.org/10.1038/s41467-022-32727-w	journal	August 2022
A scalable platform to discover antimicrobials of ribosomal origin Ayikpoe, Richard S.; Shi, Chengyou; Battiste, Alexander J. Nature Communications, Vol. 13, Issue 1 https://doi.org/10.1038/s41467-022-33890-w	journal	October 2022
Feedforward growth rate control mitigates gene activation burden Barajas, Carlos; Huang, Hsin-Ho; Gibson, Jesse Nature Communications, Vol. 13, Issue 1 https://doi.org/10.1038/s41467-022-34647-1	journal	November 2022
The evolution of antibiotic resistance is associated with collateral drug phenotypes in Mycobacterium tuberculosis Waller, Natalie J. E.; Cheung, Chen-Yi; Cook, Gregory M. Nature Communications, Vol. 14, Issue 1 https://doi.org/10.1038/s41467-023-37184-7	journal	March 2023
Disordered regions in proteusin peptides guide post-translational modification by a flavin-dependent RiPP brominase Nguyen, Nguyet A.; Vidya, F. N. U.; Yennawar, Neela H. Nature Communications, Vol. 15, Issue 1 https://doi.org/10.1038/s41467-024-45593-5	journal	February 2024
Cell-free biosynthesis and engineering of ribosomally synthesized lanthipeptides Liu, Wan-Qiu; Ji, Xiangyang; Ba, Fang Nature Communications, Vol. 15, Issue 1 https://doi.org/10.1038/s41467-024-48726-y	journal	May 2024
Assessing and harnessing updated polyketide synthase modules through combinatorial engineering Ray, Katherine A.; Lutgens, Joshua D.; Bista, Ramesh Nature Communications, Vol. 15, Issue 1 https://doi.org/10.1038/s41467-024-50844-6	journal	August 2024
Modification and de novo design of non-ribosomal peptide synthetases using specific assembly points within condensation domains Bozhüyük, Kenan A. J.; Linck, Annabell; Tietze, Andreas Nature Chemistry, Vol. 11, Issue 7 https://doi.org/10.1038/s41557-019-0276-z	journal	June 2019
Genome mining- and synthetic biology-enabled production of hypermodified peptides Bhushan, Agneya; Egli, Peter J.; Peters, Eike E. Nature Chemistry, Vol. 11, Issue 10 https://doi.org/10.1038/s41557-019-0323-9	journal	September 2019
Non-modular fatty acid synthases yield distinct N-terminal acylation in ribosomal peptides Ren, Hengqian; Huang, Chunshuai; Pan, Yuwei Nature Chemistry, Vol. 16, Issue 8 https://doi.org/10.1038/s41557-024-01491-3	journal	March 2024
CRAGE enables rapid activation of biosynthetic gene clusters in undomesticated bacteria Wang, Gaoyan; Zhao, Zhiying; Ke, Jing Nature Microbiology, Vol. 4, Issue 12 https://doi.org/10.1038/s41564-019-0573-8	journal	October 2019
Systematic mining of the human microbiome identifies antimicrobial peptides with diverse activity spectra King, Andrew M.; Zhang, Zhengan; Glassey, Emerson Nature Microbiology, Vol. 8, Issue 12 https://doi.org/10.1038/s41564-023-01524-6	journal	November 2023
Strategies to access biosynthetic novelty in bacterial genomes for drug discovery Hemmerling, Franziska; Piel, Jörn Nature Reviews Drug Discovery, Vol. 21, Issue 5 https://doi.org/10.1038/s41573-022-00414-6	journal	March 2022
Cell-free gene expression: an expanded repertoire of applications Silverman, Adam D.; Karim, Ashty S.; Jewett, Michael C. Nature Reviews Genetics, Vol. 21, Issue 3 https://doi.org/10.1038/s41576-019-0186-3	journal	November 2019
Trapping biosynthetic acyl-enzyme intermediates with encoded 2,3-diaminopropionic acid Huguenin-Dezot, Nicolas; Alonzo, Diego A.; Heberlig, Graham W. Nature, Vol. 565, Issue 7737 https://doi.org/10.1038/s41586-018-0781-z	journal	December 2018
Cell-free biosensors for rapid detection of water contaminants Jung, Jaeyoung K.; Alam, Khalid K.; Verosloff, Matthew S. Nature Biotechnology, Vol. 38, Issue 12 https://doi.org/10.1038/s41587-020-0571-7	journal	July 2020
Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale Liew, Fungmin Eric; Nogle, Robert; Abdalla, Tanus Nature Biotechnology https://doi.org/10.1038/s41587-021-01195-w	journal	February 2022
In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design Karim, Ashty S.; Dudley, Quentin M.; Juminaga, Alex Nature Chemical Biology, Vol. 16, Issue 8 https://doi.org/10.1038/s41589-020-0559-0	journal	June 2020
Enzymology of assembly line synthesis by modular polyketide synthases Grininger, Martin Nature Chemical Biology, Vol. 19, Issue 4 https://doi.org/10.1038/s41589-023-01277-7	journal	March 2023
Substrate interactions guide cyclase engineering and lasso peptide diversification Barrett, Susanna E.; Yin, Song; Jordan, Peter Nature Chemical Biology, Vol. 21, Issue 3 https://doi.org/10.1038/s41589-024-01727-w	journal	September 2024
Cell-free protein synthesis from non-growing, stressed Escherichia coli Failmezger, Jurek; Rauter, Michael; Nitschel, Robert Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-16767-7	journal	November 2017
In vitro synthesis of 32 translation-factor proteins from a single template reveals impaired ribosomal processivity Doerr, Anne; Foschepoth, David; Forster, Anthony C. Scientific Reports, Vol. 11, Issue 1 https://doi.org/10.1038/s41598-020-80827-8	journal	January 2021
Profiling expression strategies for a type III polyketide synthase in a lysate-based, cell-free system Sword, Tien T.; Dinglasan, Jaime Lorenzo N.; Abbas, Ghaeath S. K. Scientific Reports, Vol. 14, Issue 1 https://doi.org/10.1038/s41598-024-61376-w	journal	June 2024
A cell-free strategy for host-specific profiling of intracellular antibiotic sensitivity and resistance Chengan, Kameshwari; Hind, Charlotte; Stanley, Maria npj Antimicrobials and Resistance, Vol. 1, Issue 1 https://doi.org/10.1038/s44259-023-00018-z	journal	December 2023
The biosynthesis, molecular genetics and enzymology of the polyketide-derived metabolites Hill, Alison M. Nat. Prod. Rep., Vol. 23, Issue 2 https://doi.org/10.1039/B301028G	journal	January 2006
Type II polyketide synthases: gaining a deeper insight into enzymatic teamwork Hertweck, Christian; Luzhetskyy, Andriy; Rebets, Yuri Nat. Prod. Rep., Vol. 24, Issue 1 https://doi.org/10.1039/B507395M	journal	January 2007
Chemical generation and modification of peptides containing multiple dehydroalanines Morrison, Philip M.; Foley, Patrick J.; Warriner, Stuart L. Chemical Communications, Vol. 51, Issue 70 https://doi.org/10.1039/C5CC05469A	journal	January 2015
Recent advances in the biosynthesis of unusual polyketide synthase substrates Ray, Lauren; Moore, Bradley S. Natural Product Reports, Vol. 33, Issue 2 https://doi.org/10.1039/C5NP00112A	journal	January 2016
Biosynthesis of polyketides by trans-AT polyketide synthases Helfrich, Eric J. N.; Piel, Jörn Natural Product Reports, Vol. 33, Issue 2 https://doi.org/10.1039/C5NP00125K	journal	January 2016
Design and synthesis of analogues of natural products Maier, Martin E. Organic & Biomolecular Chemistry, Vol. 13, Issue 19 https://doi.org/10.1039/C5OB00169B	journal	January 2015
Cell-free extract based optimization of biomolecular circuits with droplet microfluidics Hori, Yutaka; Kantak, Chaitanya; Murray, Richard M. Lab on a Chip, Vol. 17, Issue 18 https://doi.org/10.1039/C7LC00552K	journal	January 2017
Protein–protein interactions in polyketide synthase–nonribosomal peptide synthetase hybrid assembly lines Miyanaga, Akimasa; Kudo, Fumitaka; Eguchi, Tadashi Natural Product Reports, Vol. 35, Issue 11 https://doi.org/10.1039/C8NP00022K	journal	January 2018
The architectures of iterative type I PKS and FAS Herbst, Dominik A.; Townsend, Craig A.; Maier, Timm Natural Product Reports, Vol. 35, Issue 10 https://doi.org/10.1039/C8NP00039E	journal	January 2018
Type II fatty acid and polyketide synthases: deciphering protein–protein and protein–substrate interactions Chen, Aochiu; Re, Rebecca N.; Burkart, Michael D. Natural Product Reports, Vol. 35, Issue 10 https://doi.org/10.1039/C8NP00040A	journal	January 2018
Molecular basis of methylation and chain-length programming in a fungal iterative highly reducing polyketide synthase Yang, Xiao-Long; Friedrich, Steffen; Yin, Sen Chemical Science, Vol. 10, Issue 36 https://doi.org/10.1039/C9SC03173A	journal	January 2019
New developments in RiPP discovery, enzymology and engineering Montalbán-López, Manuel; Scott, Thomas A.; Ramesh, Sangeetha Natural Product Reports, Vol. 38, Issue 1 https://doi.org/10.1039/D0NP00027B	journal	January 2021
The quest for supernatural products: the impact of total synthesis in complex natural products medicinal chemistry Wu, Zhi-Chen; Boger, Dale L. Natural Product Reports, Vol. 37, Issue 11 https://doi.org/10.1039/D0NP00060D	journal	January 2020
Streptomyces cell-free systems for natural product discovery and engineering Moore, Simon J.; Lai, Hung-En; Li, Jian Natural Product Reports, Vol. 40, Issue 2 https://doi.org/10.1039/D2NP00057A	journal	January 2023
Discovery and engineering of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products Li, He; Ding, Wei; Zhang, Qi RSC Chemical Biology, Vol. 5, Issue 2 https://doi.org/10.1039/D3CB00172E	journal	January 2024
Global genome mining-driven discovery of an unusual biosynthetic logic for fungal polyketide–terpenoid hybrids Yan, Dexiu; Matsuda, Yudai Chemical Science, Vol. 15, Issue 8 https://doi.org/10.1039/D3SC06001B	journal	January 2024
The type I fatty acid and polyketide synthases: a tale of two megasynthases Smith, Stuart; Tsai, Shiou-Chuan Natural Product Reports, Vol. 24, Issue 5 https://doi.org/10.1039/b603600g	journal	January 2007
Genomic basis for natural product biosynthetic diversity in the actinomycetes Nett, Markus; Ikeda, Haruo; Moore, Bradley S. Natural Product Reports, Vol. 26, Issue 11 https://doi.org/10.1039/b817069j	journal	January 2009
Employing a polyketide synthase module and thioesterase in the semipreparative biocatalysis of diverse triketide pyrones Hughes, Amanda J.; Detelich, Joshua F.; Keatinge-Clay, Adrian T. MedChemComm, Vol. 3, Issue 8 https://doi.org/10.1039/c2md20013a	journal	January 2012
Note on the Preparation of Yeast-jice by Buchner's Method Harden, Arthur; Henley, Francis Robert Biochemical Journal, Vol. 21, Issue 1 https://doi.org/10.1042/bj0210196	journal	January 1927
Specific, efficient, and selective inhibition of prokaryotic translation initiation by a novel peptide antibiotic Brandi, Letizia; Fabbretti, Attilio; La Teana, Anna Proceedings of the National Academy of Sciences, Vol. 103, Issue 1 https://doi.org/10.1073/pnas.0507740102	journal	December 2005
IRAK-4: A novel member of the IRAK family with the properties of an IRAK-kinase Li, S.; Strelow, A.; Fontana, E. J. Proceedings of the National Academy of Sciences, Vol. 99, Issue 8 https://doi.org/10.1073/pnas.082100399	journal	April 2002
A feeling for the numbers in biology Phillips, R.; Milo, R. Proceedings of the National Academy of Sciences, Vol. 106, Issue 51 https://doi.org/10.1073/pnas.0907732106	journal	December 2009
Temporal dynamics of natural product biosynthesis in marine cyanobacteria Esquenazi, Eduardo; Jones, Adam C.; Byrum, Tara Proceedings of the National Academy of Sciences, Vol. 108, Issue 13 https://doi.org/10.1073/pnas.1012813108	journal	March 2011
Elucidation of the anaerobic pathway for the corrin component of cobalamin (vitamin B12) Moore, S. J.; Lawrence, A. D.; Biedendieck, R. Proceedings of the National Academy of Sciences, Vol. 110, Issue 37 https://doi.org/10.1073/pnas.1308098110	journal	August 2013
Implementation of cell-free biological networks at steady state Niederholtmeyer, H.; Stepanova, V.; Maerkl, S. J. Proceedings of the National Academy of Sciences, Vol. 110, Issue 40 https://doi.org/10.1073/pnas.1311166110	journal	September 2013
Evolution of chemical diversity by coordinated gene swaps in type II polyketide gene clusters Hillenmeyer, Maureen E.; Vandova, Gergana A.; Berlew, Erin E. Proceedings of the National Academy of Sciences, Vol. 112, Issue 45 https://doi.org/10.1073/pnas.1511688112	journal	October 2015
Resistance mutations generate divergent antibiotic susceptibility profiles against translation inhibitors Cocozaki, Alexis I.; Altman, Roger B.; Huang, Jian Proceedings of the National Academy of Sciences, Vol. 113, Issue 29 https://doi.org/10.1073/pnas.1605127113	journal	July 2016
Molecular spherical nucleic acids Li, Hui; Zhang, Bohan; Lu, Xueguang Proceedings of the National Academy of Sciences, Vol. 115, Issue 17 https://doi.org/10.1073/pnas.1801836115	journal	April 2018
Ribosomally derived lipopeptides containing distinct fatty acyl moieties Hubrich, Florian; Bösch, Nina M.; Chepkirui, Clara Proceedings of the National Academy of Sciences, Vol. 119, Issue 3 https://doi.org/10.1073/pnas.2113120119	journal	January 2022
Cell-Free Peptide Synthesis Dependent upon Synthetic Oligodeoxynucleotides Leder, Philip; Clark, Brian F. C.; Sly, William S. Proceedings of the National Academy of Sciences, Vol. 50, Issue 6 https://doi.org/10.1073/pnas.50.6.1135	journal	December 1963
A functional peptide encoded in the Escherichia coli 23S rRNA. Tenson, T.; DeBlasio, A.; Mankin, A. Proceedings of the National Academy of Sciences, Vol. 93, Issue 11 https://doi.org/10.1073/pnas.93.11.5641	journal	May 1996
Biosynthesis of vitamin B ₁₂ : Factor IV, a new intermediate in the anaerobic pathway Scott, A. Ian; Stolowich, Neal J.; Wang, Jianji Proceedings of the National Academy of Sciences, Vol. 93, Issue 25 https://doi.org/10.1073/pnas.93.25.14316	journal	December 1996
Production of cyclic peptides and proteins in vivo Scott, C. P.; Abel-Santos, E.; Wall, M. Proceedings of the National Academy of Sciences, Vol. 96, Issue 24 https://doi.org/10.1073/pnas.96.24.13638	journal	November 1999
Accelerated chemical synthesis of peptides and small proteins Miranda, Les P.; Alewood, Paul F. Proceedings of the National Academy of Sciences, Vol. 96, Issue 4 https://doi.org/10.1073/pnas.96.4.1181	journal	February 1999
Ubiquitin-mediated Processing of NF-κB Transcriptional Activator Precursor p105 Orian, Amir; Whiteside, Simon; Israël, Alain Journal of Biological Chemistry, Vol. 270, Issue 37 https://doi.org/10.1074/jbc.270.37.21707	journal	September 1995
Insights into an Unusual Nonribosomal Peptide Synthetase Biosynthesis Binz, Tina M.; Maffioli, Sonia I.; Sosio, Margherita Journal of Biological Chemistry, Vol. 285, Issue 43 https://doi.org/10.1074/jbc.M110.146803	journal	October 2010
Modular optimization in metabolic engineering Wong, Matthew; Badri, Abinaya; Gasparis, Christopher Critical Reviews in Biochemistry and Molecular Biology, Vol. 56, Issue 6 https://doi.org/10.1080/10409238.2021.1937928	journal	June 2021
Antimicrobial Resistance among and Therapeutic Options against Gram‐Negative Pathogens Rahal, James J. Clinical Infectious Diseases, Vol. 49, Issue s1 https://doi.org/10.1086/599810	journal	August 2009
Timing the start of division in E. coli : a single-cell study Reshes, G.; Vanounou, S.; Fishov, I. Physical Biology, Vol. 5, Issue 4 https://doi.org/10.1088/1478-3975/5/4/046001	journal	November 2008
Rifampin: Mechanisms of Action and Resistance Wehrli, Walter Clinical Infectious Diseases, Vol. 5, Issue Supplement_3 https://doi.org/10.1093/clinids/5.Supplement_3.S407	journal	July 1983
Structural insights into simocyclinone as an antibiotic, effector ligand and substrate Buttner, Mark J.; Schäfer, Martin; Lawson, David M. FEMS Microbiology Reviews, Vol. 42, Issue 1 https://doi.org/10.1093/femsre/fux055	journal	November 2017
ResFinder 4.0 for predictions of phenotypes from genotypes Bortolaia, Valeria; Kaas, Rolf S.; Ruppe, Etienne Journal of Antimicrobial Chemotherapy, Vol. 75, Issue 12 https://doi.org/10.1093/jac/dkaa345	journal	August 2020
Identification of acquired antimicrobial resistance genes Zankari, E.; Hasman, H.; Cosentino, S. Journal of Antimicrobial Chemotherapy, Vol. 67, Issue 11 https://doi.org/10.1093/jac/dks261	journal	July 2012
Evolutionary Implications of Bacterial Polyketide Synthases Jenke-Kodama, Holger; Sandmann, Axel; Müller, Rolf Molecular Biology and Evolution, Vol. 22, Issue 10 https://doi.org/10.1093/molbev/msi193	journal	June 2005
Ubiquitous mRNA decay fragments in E. coli redefine the functional transcriptome Herzel, Lydia; Stanley, Julian A.; Yao, Chun-Chen Nucleic Acids Research, Vol. 50, Issue 9 https://doi.org/10.1093/nar/gkac295	journal	May 2022
antiSMASH 7.0: new and improved predictions for detection, regulation, chemical structures and visualisation Blin, Kai; Shaw, Simon; Augustijn, Hannah E. Nucleic Acids Research, Vol. 51, Issue W1 https://doi.org/10.1093/nar/gkad344	journal	May 2023
BioNumbers—the database of key numbers in molecular and cell biology Milo, Ron; Jorgensen, Paul; Moran, Uri Nucleic Acids Research, Vol. 38, Issue suppl_1 https://doi.org/10.1093/nar/gkp889	journal	October 2009
antiSMASH: rapid identification, annotation and analysis of secondary metabolite biosynthesis gene clusters in bacterial and fungal genome sequences Medema, Marnix H.; Blin, Kai; Cimermancic, Peter Nucleic Acids Research, Vol. 39, Issue suppl_2 https://doi.org/10.1093/nar/gkr466	journal	June 2011
GenBank Benson, Dennis A.; Cavanaugh, Mark; Clark, Karen Nucleic Acids Research, Vol. 41, Issue D1 https://doi.org/10.1093/nar/gks1195	journal	November 2012
Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology Chappell, James; Jensen, Kirsten; Freemont, Paul S. Nucleic Acids Research, Vol. 41, Issue 5 https://doi.org/10.1093/nar/gkt052	journal	January 2013
BAGEL3: automated identification of genes encoding bacteriocins and (non-)bactericidal posttranslationally modified peptides van Heel, Auke J.; de Jong, Anne; Montalbán-López, Manuel Nucleic Acids Research, Vol. 41, Issue W1 https://doi.org/10.1093/nar/gkt391	journal	May 2013
Synthetic circuit for exact adaptation and fold-change detection Kim, Jongmin; Khetarpal, Ishan; Sen, Shaunak Nucleic Acids Research, Vol. 42, Issue 9 https://doi.org/10.1093/nar/gku233	journal	April 2014
Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction Fritz, Brian R.; Jamil, Osman K.; Jewett, Michael C. Nucleic Acids Research, Vol. 43, Issue 9 https://doi.org/10.1093/nar/gkv329	journal	April 2015
RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket López-Alonso, Jorge Pedro; Kaminishi, Tatsuya; Kikuchi, Takeshi Nucleic Acids Research, Vol. 45, Issue 11 https://doi.org/10.1093/nar/gkx324	journal	May 2017
The Antibiotic Resistant Target Seeker (ARTS), an exploration engine for antibiotic cluster prioritization and novel drug target discovery Alanjary, Mohammad; Kronmiller, Brent; Adamek, Martina Nucleic Acids Research, Vol. 45, Issue W1 https://doi.org/10.1093/nar/gkx360	journal	May 2017
The effect of macromolecular crowding on single-round transcription by Escherichia coli RNA polymerase Chung, SangYoon; Lerner, Eitan; Jin, Yan Nucleic Acids Research, Vol. 47, Issue 3 https://doi.org/10.1093/nar/gky1277	journal	December 2018
Protein Synthesis in a Cell-free System from an Extreme Thermophile. Ohno-Iwashita, Yoshiko; Oshima, Tairo; Imahori, Kazutomo The Journal of Biochemistry, Vol. 79, Issue 6 https://doi.org/10.1093/oxfordjournals.jbchem.a131178	journal	June 1976
High-yield ‘one-pot’ biosynthesis of raspberry ketone, a high-value fine chemical Moore, Simon J.; Tosi, Tommaso; Bell, David Synthetic Biology, Vol. 6, Issue 1 https://doi.org/10.1093/synbio/ysab021	journal	August 2021
Development of a Pseudomonas putida cell-free protein synthesis platform for rapid screening of gene regulatory elements Wang, He; Li, Jian; Jewett, Michael C. Synthetic Biology, Vol. 3, Issue 1 https://doi.org/10.1093/synbio/ysy003	journal	January 2018
Use of a Fractionated, Coupled Transcription-Translation System in the Study of Ribosomal Resistance Mechanisms in Antibiotic-producing Streptomyces Calcutt, M. J.; Cundliffe, E. Microbiology, Vol. 135, Issue 5 https://doi.org/10.1099/00221287-135-5-1071	journal	May 1989
Nonribosomal biosynthesis of vancomycin-type antibiotics: a heptapeptide backbone and eight peptide synthetase modules The GenBank/EMBL/DDBJ accession number for the balhimycin biosynthetic gene sequence reported in this paper is Y16952. Recktenwald, Jürgen; Shawky, Riham; Puk, Oliver Microbiology, Vol. 148, Issue 4 https://doi.org/10.1099/00221287-148-4-1105	journal	April 2002
Coarse-Grained Dynamics of Protein Synthesis in a Cell-Free System Karzbrun, Eyal; Shin, Jonghyeon; Bar-Ziv, Roy H. Physical Review Letters, Vol. 106, Issue 4 https://doi.org/10.1103/PhysRevLett.106.048104	journal	January 2011
Discovering new bioactive molecules from microbial sources Monciardini, Paolo; Iorio, Marianna; Maffioli, Sonia Microbial Biotechnology, Vol. 7, Issue 3 https://doi.org/10.1111/1751-7915.12123	journal	March 2014
Oxidation of elongation factor G inhibits the synthesis of the D1 protein of photosystem II Kojima, Kouji; Oshita, Masaru; Nanjo, Yohei Molecular Microbiology, Vol. 65, Issue 4 https://doi.org/10.1111/j.1365-2958.2007.05836.x	journal	August 2007
Effects of Apramycin, a Novel Aminoglycoside Antibiotic on Bacterial Protein Synthesis Perzynski, Stanislaw; Cannon, Michael; Cundliffe, Eric European Journal of Biochemistry, Vol. 99, Issue 3 https://doi.org/10.1111/j.1432-1033.1979.tb13295.x	journal	September 1979
Cloning and expression in Escherichia coli of a hygromycin B phosphotransferase gene from Streptomyces hygroscopicus Zalacain, Magdalena; Malpartida, Francisco; Pulido, Diego European Journal of Biochemistry, Vol. 162, Issue 2 https://doi.org/10.1111/j.1432-1033.1987.tb10617.x	journal	January 1987
Mechanism of resistance to the antibiotic trichothecin in the producing fungi Iglesias, Maite; Ballesta, Juan P. G. European Journal of Biochemistry, Vol. 223, Issue 2 https://doi.org/10.1111/j.1432-1033.1994.tb19012.x	journal	July 1994
Effects of Escherichia coli ribosomal protein S12 mutations on cell‐free protein synthesis Chumpolkulwong, Namthip; Hori‐Takemoto, Chie; Hosaka, Takeshi European Journal of Biochemistry, Vol. 271, Issue 6 https://doi.org/10.1111/j.1432-1033.2004.04016.x	journal	February 2004
Differential activity profiles of translation inhibitors in whole-cell and cell-free approaches Weidlich, M.; Klammt, C.; Bernhard, F. Letters in Applied Microbiology, Vol. 46, Issue 2 https://doi.org/10.1111/j.1472-765X.2007.02281.x	journal	November 2007
An inventory of the bacterial macromolecular components and their spatial organization Vendeville, Agnès; Larivière, Damien; Fourmentin, Eric FEMS Microbiology Reviews, Vol. 35, Issue 2 https://doi.org/10.1111/j.1574-6976.2010.00254.x	journal	March 2011
PRODUCTION OF ANTIBIOTIC SUBSTANCES BY ACTINOMYCETES*^† Waksman, Selman A.; Schatz, Albert; Reynolds, Donald M. Annals of the New York Academy of Sciences, Vol. 1213, Issue 1 https://doi.org/10.1111/j.1749-6632.2010.05861.x	journal	December 2010
Metabolic engineering of cobalamin (vitamin B₁₂) production in Bacillus megaterium Biedendieck, Rebekka; Malten, Marco; Barg, Heiko Microbial Biotechnology, Vol. 3, Issue 1 https://doi.org/10.1111/j.1751-7915.2009.00125.x	journal	December 2009
Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters: Streptomyces host for heterologous expression of gene clusters Gomez-Escribano, Juan Pablo; Bibb, Mervyn J. Microbial Biotechnology, Vol. 4, Issue 2 https://doi.org/10.1111/j.1751-7915.2010.00219.x	journal	October 2010
Permeability barriers of Gram‐negative pathogens Zgurskaya, Helen I.; Rybenkov, Valentin V. Annals of the New York Academy of Sciences, Vol. 1459, Issue 1 https://doi.org/10.1111/nyas.14134	journal	June 2019
BioBits™ Explorer: A modular synthetic biology education kit Huang, Ally; Nguyen, Peter Q.; Stark, Jessica C. Science Advances, Vol. 4, Issue 8 https://doi.org/10.1126/sciadv.aat5105	journal	August 2018
Biosynthesis of Complex Polyketides in a Metabolically Engineered Strain of E. coli Pfeifer, Blaine A.; Admiraal, Suzanne J.; Gramajo, Hugo Science, Vol. 291, Issue 5509, p. 1790-1792 https://doi.org/10.1126/science.1058092	journal	March 2001
Structure and Mechanism of the Lantibiotic Cyclase Involved in Nisin Biosynthesis Li, B. Science, Vol. 311, Issue 5766 https://doi.org/10.1126/science.1121422	journal	March 2006
Metagenome Mining Reveals Polytheonamides as Posttranslationally Modified Ribosomal Peptides Freeman, M. F.; Gurgui, C.; Helf, M. J. Science, Vol. 338, Issue 6105 https://doi.org/10.1126/science.1226121	journal	September 2012
Fate of a Synthetic Polynucleotide Directing Cell-Free Protein Synthesis II. Association with Ribosomes Barondes, Samuel H.; Nirenberg, Marshall W. Science, Vol. 138, Issue 3542 https://doi.org/10.1126/science.138.3542.813	journal	November 1962
Use of a scaffold peptide in the biosynthesis of amino acid–derived natural products Ting, Chi P.; Funk, Michael A.; Halaby, Steve L. Science, Vol. 365, Issue 6450 https://doi.org/10.1126/science.aau6232	journal	July 2019
Synthesis of proteins by automated flow chemistry Hartrampf, N.; Saebi, A.; Poskus, M. Science, Vol. 368, Issue 6494 https://doi.org/10.1126/science.abb2491	journal	May 2020
Evolution-inspired engineering of nonribosomal peptide synthetases Bozhüyük, Kenan A. J.; Präve, Leonard; Kegler, Carsten Science, Vol. 383, Issue 6689 https://doi.org/10.1126/science.adg4320	journal	March 2024
Evolution-guided engineering of trans -acyltransferase polyketide synthases Mabesoone, Mathijs F. J.; Leopold-Messer, Stefan; Minas, Hannah A. Science, Vol. 383, Issue 6689 https://doi.org/10.1126/science.adj7621	journal	March 2024
rRNA Binding Sites and the Molecular Mechanism of Action of the Tetracyclines Chukwudi, Chinwe U. Antimicrobial Agents and Chemotherapy, Vol. 60, Issue 8 https://doi.org/10.1128/AAC.00594-16	journal	August 2016
Quinolone-Mediated Bacterial Death Drlica, K.; Malik, M.; Kerns, R. J. Antimicrobial Agents and Chemotherapy, Vol. 52, Issue 2 https://doi.org/10.1128/AAC.01617-06	journal	August 2007
Polysome Metabolism in Escherichia coli: Amicetin, an Antibiotic That Stabilizes Polysomes Ennis, Herbert L. Antimicrobial Agents and Chemotherapy, Vol. 1, Issue 3 https://doi.org/10.1128/AAC.1.3.204	journal	March 1972
Molecular basis of tetracycline action: identification of analogs whose primary target is not the bacterial ribosome Rasmussen, B.; Noller, H. F.; Daubresse, G. Antimicrobial Agents and Chemotherapy, Vol. 35, Issue 11 https://doi.org/10.1128/AAC.35.11.2306	journal	November 1991
Mechanism of action of oxazolidinones: effects of linezolid and eperezolid on translation reactions Shinabarger, D. L.; Marotti, K. R.; Murray, R. W. Antimicrobial Agents and Chemotherapy, Vol. 41, Issue 10 https://doi.org/10.1128/AAC.41.10.2132	journal	October 1997
Staphylococcus aureus Cell Extract Transcription-Translation Assay: Firefly Luciferase Reporter System for Evaluating Protein Translation Inhibitors Murray, Robert W.; Melchior, Earline P.; Hagadorn, Jeanne C. Antimicrobial Agents and Chemotherapy, Vol. 45, Issue 6 https://doi.org/10.1128/AAC.45.6.1900-1904.2001	journal	June 2001
Structure-Guided Discovery of Novel Aminoglycoside Mimetics as Antibacterial Translation Inhibitors Zhou, Yuefen; Gregor, Vlad E.; Sun, Zhongxiang Antimicrobial Agents and Chemotherapy, Vol. 49, Issue 12 https://doi.org/10.1128/AAC.49.12.4942-4949.2005	journal	December 2005
Simocyclinone D8, an Inhibitor of DNA Gyrase with a Novel Mode of Action Flatman, Ruth H.; Howells, Alison J.; Heide, Lutz Antimicrobial Agents and Chemotherapy, Vol. 49, Issue 3 https://doi.org/10.1128/AAC.49.3.1093-1100.2005	journal	March 2005
Selman A. Waksman, Winner of the 1952 Nobel Prize for Physiology or Medicine Woodruff, H. Boyd Applied and Environmental Microbiology, Vol. 80, Issue 1 https://doi.org/10.1128/AEM.01143-13	journal	January 2014
In Vivo Production of Artificial Nonribosomal Peptide Products in the Heterologous Host Escherichia coli Gruenewald, Stephan; Mootz, Henning D.; Stehmeier, Per Applied and Environmental Microbiology, Vol. 70, Issue 6 https://doi.org/10.1128/AEM.70.6.3282-3291.2004	journal	June 2004
Challenges of Antibacterial Discovery Silver, L. L. Clinical Microbiology Reviews, Vol. 24, Issue 1 https://doi.org/10.1128/CMR.00030-10	journal	January 2011
Protein Mobility in the Cytoplasm ofEscherichia coli Elowitz, Michael B.; Surette, Michael G.; Wolf, Pierre-Etienne Journal of Bacteriology, Vol. 181, Issue 1 https://doi.org/10.1128/JB.181.1.197-203.1999	journal	January 1999
Modulation of Chemical Composition and Other Parameters of the Cell at Different Exponential Growth Rates Dennis, Patrick P.; Bremer, Hans EcoSal Plus, Vol. 3, Issue 1 https://doi.org/10.1128/ecosal.5.2.3	journal	September 2008
Draft Genome Sequence of the Fast-Growing Marine Bacterium Vibrio natriegens Strain ATCC 14048 Wang, Zheng; Lin, Baochuan; Hervey, W. Judson Genome Announcements, Vol. 1, Issue 4 https://doi.org/10.1128/genomeA.00589-13	journal	August 2013
Draft Genome Sequence of the Fast-Growing Bacterium Vibrio natriegens Strain DSMZ 759 Maida, I.; Bosi, E.; Perrin, E. Genome Announcements, Vol. 1, Issue 4 https://doi.org/10.1128/genomeA.00648-13	journal	June 2013
Establishment of exponential growth after a nutritional shift-up in Escherichia coli B/r: accumulation of deoxyribonucleic acid, ribonucleic acid, and protein. Brunschede, H.; Dove, T. L.; Bremer, H. Journal of Bacteriology, Vol. 129, Issue 2 https://doi.org/10.1128/jb.129.2.1020-1033.1977	journal	January 1977
A 24-amino-acid polypeptide is essential for the biosynthesis of the coenzyme pyrrolo-quinoline-quinone Goosen, N.; Huinen, R. G.; van de Putte, P. Journal of Bacteriology, Vol. 174, Issue 4 https://doi.org/10.1128/jb.174.4.1426-1427.1992	journal	February 1992
Tetracycline Inhibition of Cell-Free Protein Synthesis I. Binding of Tetracycline to Components of the System Day, L. E. Journal of Bacteriology, Vol. 91, Issue 5 https://doi.org/10.1128/jb.91.5.1917-1923.1966	journal	May 1966
Cryptic or Silent? The Known Unknowns, Unknown Knowns, and Unknown Unknowns of Secondary Metabolism Hoskisson, Paul A.; Seipke, Ryan F. mBio, Vol. 11, Issue 5 https://doi.org/10.1128/mBio.02642-20	journal	October 2020
Dual-Uptake Mode of the Antibiotic Phazolicin Prevents Resistance Acquisition by Gram-Negative Bacteria Travin, Dmitrii Y.; Jouan, Romain; Vigouroux, Armelle mBio, Vol. 14, Issue 2 https://doi.org/10.1128/mbio.00217-23	journal	April 2023
Full Profiling of GE81112A, an Underexplored Tetrapeptide Antibiotic with Activity against Gram-Negative Pathogens Schuler, Sören M. M.; Jürjens, Gerrit; Marker, Alexander Microbiology Spectrum, Vol. 11, Issue 3 https://doi.org/10.1128/spectrum.02247-22	journal	June 2023
Triumphs and Challenges of Natural Product Discovery in the Postgenomic Era Cano-Prieto, Carolina; Undabarrena, Agustina; de Carvalho, Ana Calheiros Annual Review of Biochemistry, Vol. 93, Issue 1 https://doi.org/10.1146/annurev-biochem-032620-104731	journal	August 2024
Structure and Mechanisms of Assembly-Line Polyketide Synthases Soohoo, Alexander M.; Cogan, Dillon P.; Brodsky, Krystal L. Annual Review of Biochemistry, Vol. 93, Issue 1 https://doi.org/10.1146/annurev-biochem-080923-043654	journal	August 2024
Drug discovery in academia Verkman, A. S. American Journal of Physiology-Cell Physiology, Vol. 286, Issue 3 https://doi.org/10.1152/ajpcell.00397.2003	journal	March 2004
A Strategy for Discovery of Novel Broad-Spectrum Antibacterials Using a High-Throughput Streptococcus pneumoniae Transcription/Translation Screen Pratt, Steven D.; David, Caroline A.; Black-Schaefer, Candace SLAS Discovery, Vol. 9, Issue 1 https://doi.org/10.1177/1087057103260876	journal	February 2004
Bioinformatic evidence for a widely distributed, ribosomally produced electron carrier precursor, its maturation proteins, and its nicotinoprotein redox partners Haft, Daniel H. BMC Genomics, Vol. 12, Issue 1 https://doi.org/10.1186/1471-2164-12-21	journal	January 2011
PKMiner: a database for exploring type II polyketide synthases Kim, Jinki; Yi, Gwan-Su BMC Microbiology, Vol. 12, Issue 1 https://doi.org/10.1186/1471-2180-12-169	journal	January 2012
Cell-free protein synthesis energized by slowly-metabolized maltodextrin Wang, Yiran; Zhang, Y-H Percival BMC Biotechnology, Vol. 9, Issue 1 https://doi.org/10.1186/1472-6750-9-58	journal	January 2009
Expansion of ribosomally produced natural products: a nitrile hydratase- and Nif11-related precursor family Haft, Daniel H.; Basu, Malay Kumar; Mitchell, Douglas A. BMC Biology, Vol. 8, Issue 1 https://doi.org/10.1186/1741-7007-8-70	journal	May 2010
A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes Thanapipatsiri, Anyarat; Claesen, Jan; Gomez-Escribano, Juan-Pablo Microbial Cell Factories, Vol. 14, Issue 1 https://doi.org/10.1186/s12934-015-0335-0	journal	September 2015
Designed biosynthesis of 25-methyl and 25-ethyl ivermectin with enhanced insecticidal activity by domain swap of avermectin polyketide synthase Zhang, Ji; Yan, Yi-Jun; An, Jing Microbial Cell Factories, Vol. 14, Issue 1 https://doi.org/10.1186/s12934-015-0337-y	journal	September 2015
Refactoring of a synthetic raspberry ketone pathway with EcoFlex Moore, Simon J.; Hleba, Yonek B.; Bischoff, Sarah Microbial Cell Factories, Vol. 20, Issue 1 https://doi.org/10.1186/s12934-021-01604-4	journal	June 2021
Thermostable in vitro transcription-translation compatible with microfluidic droplets Ribeiro, Ana L. J. L.; Pérez-Arnaiz, Patricia; Sánchez-Costa, Mercedes Microbial Cell Factories, Vol. 23, Issue 1 https://doi.org/10.1186/s12934-024-02440-y	journal	June 2024
Review of deep learning: concepts, CNN architectures, challenges, applications, future directions Alzubaidi, Laith; Zhang, Jinglan; Humaidi, Amjad J. Journal of Big Data, Vol. 8, Issue 1 https://doi.org/10.1186/s40537-021-00444-8	journal	March 2021
Characterization of GE82832, a peptide inhibitor of translocation interacting with bacterial 30S ribosomal subunits Brandi, Letizia; Fabbretti, Attilio; Stefano, Michele Di RNA, Vol. 12, Issue 7 https://doi.org/10.1261/rna.61206	journal	May 2006
Will 10 Million People Die a Year due to Antimicrobial Resistance by 2050? de Kraker, Marlieke E. A.; Stewardson, Andrew J.; Harbarth, Stephan PLOS Medicine, Vol. 13, Issue 11 https://doi.org/10.1371/journal.pmed.1002184	journal	November 2016
Valinomycin Biosynthetic Gene Cluster in Streptomyces: Conservation, Ecology and Evolution Matter, Andrea M.; Hoot, Sara B.; Anderson, Patrick D. PLoS ONE, Vol. 4, Issue 9 https://doi.org/10.1371/journal.pone.0007194	journal	September 2009
Cell-Free Protein Expression under Macromolecular Crowding Conditions Ge, Xumeng; Luo, Dan; Xu, Jianfeng PLoS ONE, Vol. 6, Issue 12 https://doi.org/10.1371/journal.pone.0028707	journal	December 2011
Artificial Environments for the Co-Translational Stabilization of Cell-Free Expressed Proteins Kai, Lei; Dötsch, Volker; Kaldenhoff, Ralf PLoS ONE, Vol. 8, Issue 2 https://doi.org/10.1371/journal.pone.0056637	journal	February 2013
Improved Cell-Free RNA and Protein Synthesis System Li, Jun; Gu, Liangcai; Aach, John PLoS ONE, Vol. 9, Issue 9 https://doi.org/10.1371/journal.pone.0106232	journal	September 2014
Multiplex transcriptional characterizations across diverse bacterial species using cell‐free systems Yim, Sung Sun; Johns, Nathan I.; Park, Jimin Molecular Systems Biology, Vol. 15, Issue 8 https://doi.org/10.15252/msb.20198875	journal	August 2019
Protein-Protein Association in Polymer Solutions: From Dilute to Semidilute to Concentrated Kozer, Noga; Kuttner, Yosef Yehuda; Haran, Gilad Biophysical Journal, Vol. 92, Issue 6 https://doi.org/10.1529/biophysj.106.097717	journal	March 2007
Mechanism of Action of Streptogramins and Macrolides Vannuffel, Pascal; Cocito, Carlo Drugs, Vol. 51, Issue Supplement 1 https://doi.org/10.2165/00003495-199600511-00006	journal	January 1996
Quinolones: Action and Resistance Updated Drlica, Karl; Hiasa, Hiroshi; Kerns, Robert Current Topics in Medicinal Chemistry, Vol. 9, Issue 11 https://doi.org/10.2174/156802609789630947	journal	August 2009
Underexplored bacteria as reservoirs of novel antimicrobial lipopeptides Clements-Decker, Tanya; Kode, Megan; Khan, Sehaam Frontiers in Chemistry, Vol. 10 https://doi.org/10.3389/fchem.2022.1025979	journal	October 2022
A Brief History of the Antibiotic Era: Lessons Learned and Challenges for the Future Aminov, Rustam I. Frontiers in Microbiology, Vol. 1 https://doi.org/10.3389/fmicb.2010.00134	journal	January 2010
Inclusion bodies: not that bad… Ramón, Ana; Señorale-Pose, Mario; Marín, Mónica Frontiers in Microbiology, Vol. 5 https://doi.org/10.3389/fmicb.2014.00056	journal	January 2014
Cell-Free Protein Synthesis From Fast-Growing Vibrio natriegens Failmezger, Jurek; Scholz, Steffen; Blombach, Bastian Frontiers in Microbiology, Vol. 9 https://doi.org/10.3389/fmicb.2018.01146	journal	June 2018
Circular and Leaderless Bacteriocins: Biosynthesis, Mode of Action, Applications, and Prospects Perez, Rodney H.; Zendo, Takeshi; Sonomoto, Kenji Frontiers in Microbiology, Vol. 9 https://doi.org/10.3389/fmicb.2018.02085	journal	September 2018
Allogenous Selection of Mutational Collateral Resistance: Old Drugs Select for New Resistance Within Antibiotic Families Baquero, Fernando; Martínez, José L.; Novais, Ângela Frontiers in Microbiology, Vol. 12 https://doi.org/10.3389/fmicb.2021.757833	journal	October 2021
In vitro and in vivo production and split-intein mediated ligation (SIML) of circular bacteriocins Peña, Nuria; Bland, Michael J.; Sevillano, Ester Frontiers in Microbiology, Vol. 13 https://doi.org/10.3389/fmicb.2022.1052686	journal	November 2022
Cell-free protein synthesis for nonribosomal peptide synthetic biology Sword, Tien T.; Abbas, Ghaeath S. K.; Bailey, Constance B. Frontiers in Natural Products, Vol. 3 https://doi.org/10.3389/fntpr.2024.1353362	journal	February 2024
An Overview of the Medicinally Important Plant Type III PKS Derived Polyketides Bisht, Renu; Bhattacharyya, Aniket; Shrivastava, Ankita Frontiers in Plant Science, Vol. 12 https://doi.org/10.3389/fpls.2021.746908	journal	October 2021
Plant-Derived Cell-Free Biofactories for the Production of Secondary Metabolites Buntru, Matthias; Hahnengress, Nils; Croon, Alexander Frontiers in Plant Science, Vol. 12 https://doi.org/10.3389/fpls.2021.794999	journal	January 2022
The Oligopeptide Permease Opp Mediates Illicit Transport of the Bacterial P-site Decoding Inhibitor GE81112 Maio, Alessandro; Brandi, Letizia; Donadio, Stefano Antibiotics, Vol. 5, Issue 2 https://doi.org/10.3390/antibiotics5020017	journal	May 2016
Triaging of Culture Conditions for Enhanced Secondary Metabolite Diversity from Different Bacteria Schwarz, Jenny; Hubmann, Georg; Rosenthal, Katrin Biomolecules, Vol. 11, Issue 2 https://doi.org/10.3390/biom11020193	journal	January 2021
Current and Emerging Methods of Antibiotic Susceptibility Testing Khan, Zeeshan A.; Siddiqui, Mohd F.; Park, Seungkyung Diagnostics, Vol. 9, Issue 2 https://doi.org/10.3390/diagnostics9020049	journal	May 2019
Analysis of the Innovation Trend in Cell-Free Synthetic Biology Meyer, Conary; Nakamura, Yusuke; Rasor, Blake J. Life, Vol. 11, Issue 6 https://doi.org/10.3390/life11060551	journal	June 2021
A User’s Guide to Cell-Free Protein Synthesis Gregorio, Nicole E.; Levine, Max Z.; Oza, Javin P. Methods and Protocols, Vol. 2, Issue 1 https://doi.org/10.3390/mps2010024	journal	March 2019
Cell-Free PURE System: Evolution and Achievements Cui, Yi; Chen, Xinjie; Wang, Ze BioDesign Research, Vol. 2022 https://doi.org/10.34133/2022/9847014	journal	January 2022
TLR10 and Its Unique Anti-Inflammatory Properties and Potential Use as a Target in Therapeutics Fore, Faith; Indriputri, Cut; Mamutse, Janet Immune Network, Vol. 20, Issue 3 https://doi.org/10.4110/in.2020.20.e21	journal	January 2020
Enzymatic and chemical modifications of lipopeptide antibiotic A21978C: The synthesis and evaluation of daptomycin (LY146032). Debono, Manuel; Abbott, Bernard J.; Molloy, R. Michael The Journal of Antibiotics, Vol. 41, Issue 8 https://doi.org/10.7164/antibiotics.41.1093	journal	January 1988
Rapid cell-free forward engineering of novel genetic ring oscillators Niederholtmeyer, Henrike; Sun, Zachary Z.; Hori, Yutaka eLife, Vol. 4 https://doi.org/10.7554/eLife.09771	journal	October 2015

Similar Records

Unnatural biosynthesis by an engineered microorganism with heterologously expressed natural enzymes and an artificial metalloenzyme

Journal Article · Wed Oct 13 20:00:00 EDT 2021 · Nature Chemistry · OSTI ID:2469700

Novel soil bacteria possess diverse genes for secondary metabolite biosynthesis

Journal Article · Tue Jun 12 20:00:00 EDT 2018 · Nature (London) · OSTI ID:1477308

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES

Cell-free synthetic biology for natural product biosynthesis and discovery

Citation Formats

References (430)

Similar Records

Related Subjects