Next-generation sequencing dataset of genome-scale CRISPRi in Synechococcus sp. PCC 7002 across seven conditions
- Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO, 80304, USA
- Biosciences Division Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA
- Department of Biochemistry, University of Colorado, Boulder, CO, 80309, USA Renewable and Sustainable Energy Institute, University of Colorado, Boulder, CO, 80309, USA National Renewable Energy Laboratory, Golden, CO, 80401, USA
A 33,298-member sgRNA library developed for Synechococus sp. PCC 7002 was screened with two replicates across seven growth conditions and sequenced with Illumina NextSeq (paired end, 2x150 bp) for a total of ~700M reads. The original plasmid library and the library after transformation into a dCas9-containing and dCas9-absent strain were also sequenced as a reference for initial sgRNA abundance.
- Research Organization:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Earth & Environmental Systems Science (EESS)
- DOE Contract Number:
- SC0018368
- OSTI ID:
- 2525933
- Country of Publication:
- United States
- Language:
- English