Preparation of coenzyme F430 biosynthetic enzymes and intermediates

Ray, Prosenjit; Rand-Fleming, Chelsea R.; Mansoorabadi, Steven O.

doi:10.1016/bs.mie.2024.06.008

Preparation of coenzyme F430 biosynthetic enzymes and intermediates

Journal Article · Sat Jul 20 00:00:00 EDT 2024 · Methods in Enzymology

DOI:https://doi.org/10.1016/bs.mie.2024.06.008· OSTI ID:2525280

Ray, Prosenjit ^[1]; Rand-Fleming, Chelsea R. ^[1]; Mansoorabadi, Steven O. ^[1]

Auburn University, AL (United States)

Methyl-coenzyme M reductase (MCR) is the key enzyme in pathways for the formation and anaerobic oxidation of methane. As methane is a potent greenhouse gas and biofuel, investigations of MCR catalysis and maturation are of interest for the development of both methanogenesis inhibitors and natural gas conversion strategies. Here, the activity of MCR is dependent on a unique, nickel-containing coenzyme F430, the most highly reduced tetrapyrrole found in nature. Coenzyme F430 is biosynthesized from sirohydrochlorin in four steps catalyzed by the CfbABCDE enzymes. Here, methods for the expression and purification of the coenzyme F430 biosynthesis enzymes are described along with conditions for the synthesis and purification of biosynthetic intermediates on the milligram scale from commercially available porphobilinogen.

View Accepted Manuscript (DOE)

Research Organization:: Auburn University, AL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0023451

OSTI ID:: 2525280

Journal Information:: Methods in Enzymology, Journal Name: Methods in Enzymology Vol. 702; ISSN 0076-6879

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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