PPI DataHub Project Data Package: S. elongatus PCC 7942 Circadian Control Bioproduction Metabolomics (PB-DP5)
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); PNNL; Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
The purpose of this experiment was to evaluate how circadian clock regulation impacts carbon partitioning between storage, growth, and product synthesis in Synechococcus elongatus PCC 7942 in providing insights to strategies for enhanced bioproduction. Culture samples were collected at 0, 0.5, 1, 2, 4, 6, and 8 hours for extracellular sucrose analysis. Circadian metabolomics data was acquired using a Agilent single quadrupole gas chromatography-mass spectrometer and processed using Agilent Mass Hunter for targeted sucrose quantification. Metabolomic analysis of PCC 7942 light-dark cycle cultures transitioned to constant light revealed distinct temporal patterns in sucrose production. Processed metabolomic datasets are openly accessible from the PNNL DataHub project dataset download page and contain secondary processed GC-MS results files and supporting metadata materials linked to relevant source code information supporting data transparency and reuse.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC)
- Contributing Organization:
- Predictive Phenomics Initiative, Pacific Northwest National Laboratory
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 2510499
- Report Number(s):
- PNNL-SA-208061; MSV000096722
- Country of Publication:
- United States
- Language:
- English
| MassIVE MSV000096722 - Cyanobacterial circadian regulation enhances bioproduction under subjective nighttime through rewiring of carbon partitioning dynamics, redox balance orchestration, and cell cycle modulation | dataset | January 2024 |
Predictive Phenomics Initiative Project Dataset Catalog Collection
|
dataset | January 2024 |
Similar Records
PPI DataHub Project Data Package: S. elongatus PCC 7942 Circadian Control Bioproduction Transcriptomics (PB-DP3)
PPI DataHub Project Data Package: S. elongatus PCC 7942 Limited Proteolysis and Thermal Proteome Profiling Structural Proteomics (JM-PB-DP3)
Dataset
·
Sun Dec 29 23:00:00 EST 2024
·
OSTI ID:2510500
PPI DataHub Project Data Package: S. elongatus PCC 7942 Limited Proteolysis and Thermal Proteome Profiling Structural Proteomics (JM-PB-DP3)
Dataset
·
Thu Jul 31 00:00:00 EDT 2025
·
OSTI ID:2574396