Mapping of the human autoantigen pericentriolar material 1 (PCM1) gene to chromosome 8p21.3-p22
Journal Article
·
· Genomics
OSTI ID:250274
- Cancer Institute, Tokyo (Japan); and others
Cosmid DNAs were digested with Bg/II and BamHI and ligated into a BamHI site of an exon trapping-vector pSPL1. Transfection of the subcloned DNAs into cos7 cells, isolation of cytoplasmic RNA, synthesis of cDNA by reverse transcriptase, and amplificaiton of spliced fragments were performed according to the methods described by Buckler et al.
- OSTI ID:
- 250274
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 24; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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