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The gene encoding the VP16-accessory protein HCF (HCFC1) resides in human Xq28 and is highly expressed in fetal tissues and the adult kidney

Journal Article · · Genomics
;  [1];  [2];  [3]
  1. Cold Spring Harbor, New York, NY (United States)
  2. Baylor College of Medicine, Houston, TX (United States)
  3. Univ. of Washington, Seattle, WA (United States); and others

After herpes simplex virus (HSV) infection, the viral regulatory protein VP16 activates transcription of the HSV immediate-early promoters by directing complex formation with two cellular proteins, the POU-homeodomain transcription factor Oct-1 and the host cell factor HCF. The function of HCF in uninfected cells is unknown. Here we show by fluorescence in situ hybridization and somatic cell hybrid analysis that the gene encoding human HCF, HCFC1, maps to the q28 region of the X chromosome. Yeast artificial chromosome and cosmid mapping localizes the HCFC1 gene within 100 kb distal of the renal vasopressin type-2 receptor (V2R) gene and adjacent to the renin-binding protein gene (RENBP). The HCFC1 gene is apparently unique. HCF transcripts and protein are most abundant in fetal and placental tissues and cell lines, suggesting a role in cell proliferation. In adults, HCF protein is abundant in the kidney, but not in the brain, a site of latent HSV infection and where HCF levels may influence progression of HSV infection. 42 refs., 3 figs.

DOE Contract Number:
FG05-92ER61401
OSTI ID:
250159
Journal Information:
Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 25; ISSN 0888-7543; ISSN GNMCEP
Country of Publication:
United States
Language:
English