Rapid mapping of NotI linking clones with differential hybridization and Alu-PCR

Journal Article · Wed Jun 01 04:00:00 EDT 1994 · Genomics

DOI:https://doi.org/10.1006/geno.1994.1305· OSTI ID:250044

Zabarovsky, E R ^[1]; Zabarovska, V I; Klein, G ^[2]

---

1. Karolinska Institute, Stockholm (Sweden)
2. Karolinska Institute, Stockholm (Sweden); and others

For construction of a NotI restriction map of the humane genome, the isolation and mapping of unique NotI linking clones represent important and critical steps. Recently the authors have shown that an Alu-PCR approach can be used for isolation of NotI linking clones from defined regions of the chromosomes. This represents a useful method for isolating and analyzing a small number of clones, but it would be laborious to use it for mapping many NotI linking clones simultaneously. Here they suggest another modification of Alu-PCR for rapid concurrent mapping of many NotI linking clones. The results clearly demonstrate the utility of this approach. Seventy-one random NotI linking clones were analyzed. Among them, 65 clones (91.5%) were correctly selected and mapped using this approach. With differential hybridization and Alu-PCR, a significant portion of all human NotI linking clones (>30%) can be rapidly mapped to particular chromosomes or to defined regions of these chromosomes. 31 refs., 3 figs.

• OSTI ID: 250044
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 21; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA HYBRIDIZATION
GENETIC MAPPING
HUMAN CHROMOSOMES
OPTIMIZATION
POLYMERASE CHAIN REACTION