Selection of chromosome 22-specific clones from human genomic BAC library using a chromosome-specific cosmid library pool

Journal Article · Fri Jul 15 04:00:00 EDT 1994 · Genomics

DOI:https://doi.org/10.1006/geno.1994.1392· OSTI ID:249990

Kim, U J; Shizuya, H; Birren, B ^[1]

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1. Lawrence Livermore National Lab., CA (United States); and others

A new approach to rapidly identify chromosome-specific subsets of clones from a total human genomic library is described. The authors report here the results of screening a human bacterial artificial chromosome (BAC) library using the total pool of clones from a chromosome 22-specific cosmid library as a composite probe. The human BAC library was gridded on filters at high density and hybridized with DNA from the pooled chromosome 22-specific Lawrist library under suppressive conditions. In a single hybridization, they picked 280 candidates from the BAC library representing over 30,000 clones (or 1.2 x coverage of human genome). This subset contained more than 60% of the chromosome 22-specific BAC clones that were previously found to be present in the original BAC library. In principle, this approach can be applied to select a subset of clones from other global libraries with relatively large inserts using a pool from a regional library as a composite probe. It is important to note that the target and probe libraries must be based on vectors that share no homology with each other. 8 refs., 2 figs., 2 tabs.

• OSTI ID: 249990
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 22; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA HYBRIDIZATION
DNA-CLONING
GENETIC MAPPING
HUMAN CHROMOSOME 22
OPTIMIZATION