Redefining the product portfolio of oilcane bagasse biorefinery: Recovering natural colorants, vegetative lipids and sugars

Banerjee, Shivali; Beraja, Galit; Eilts, Kristen K.; Singh, Vijay

doi:10.1016/j.biortech.2025.132052

Redefining the product portfolio of oilcane bagasse biorefinery: Recovering natural colorants, vegetative lipids and sugars

Journal Article · Thu Jan 09 00:00:00 EST 2025 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2025.132052· OSTI ID:2497857

^[1]; Beraja, Galit ^[1]; ^[1]; ^[1]

University of Illinois at Urbana-Champaign, IL (United States)

Bioenergy crops have been known for their ability to produce biofuels and bioproducts. In this study, the product portfolio of recently developed transgenic sugarcane (oilcane) bagasse has been redefined for recovering natural pigments (anthocyanins), sugars, and vegetative lipids. The total anthocyanin content in oilcane bagasse has been estimated as 92.9 ± 18.9 µg/g of dried bagasse with cyanidin-3-glucoside (13.5 ± 18.9 µg per g of dried bagasse) as the most prominent anthocyanin present. More than 85 % (w/w) of the total anthocyanins were recovered from oilcane bagasse at a pretreatment temperature of 150 °C for 15 min. These conditions for the hydrothermal pretreatment also led to a 2-fold increase in the glucose yield upon the enzymatic saccharification of the pretreated bagasse. Further, a 1.5-fold enrichment of the vegetative lipids was demonstrated in the pretreated residue. Re-defining green biorefineries with multiple high-value products in a zero-waste approach is the need of the hour for attaining sustainability.

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Research Organization:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018420

OSTI ID:: 2497857

Alternate ID(s):: OSTI ID: 2502089

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Vol. 419; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (58)

Recent advances in the integrated biorefinery concept for the valorization of algal biomass through sustainable routes Katiyar, Richa; Banerjee, Shivali; Arora, Amit Biofuels, Bioproducts and Biorefining, Vol. 15, Issue 3 https://doi.org/10.1002/bbb.2187	journal	February 2021
Determination and comparison of flavonoids and anthocyanins in Chinese sugarcane tips, stems, roots and leaves Li, Xinying; Yao, Shun; Tu, Bin Journal of Separation Science, Vol. 33, Issue 9 https://doi.org/10.1002/jssc.200900567	journal	March 2010
Integrated Biorefinery Strategy for Valorization of Pineapple Processing Waste into High-Value Products Banerjee, Shivali; Vijayaraghavan, R.; Patti, Antonio F. Waste and Biomass Valorization, Vol. 13, Issue 1 https://doi.org/10.1007/s12649-021-01542-7	journal	August 2021
Continuous subcritical water extraction as a useful tool for isolation of edible essential oils Soto Ayala, Rogelio; Luque de Castro, M. D. Food Chemistry, Vol. 75, Issue 1 https://doi.org/10.1016/S0308-8146(01)00212-6	journal	October 2001
Pressurized Hot Water Extraction of anthocyanins from red onion: A study on extraction and degradation rates Petersson, Erik V.; Liu, Jiayin; Sjöberg, Per J. R. Analytica Chimica Acta, Vol. 663, Issue 1 https://doi.org/10.1016/j.aca.2010.01.023	journal	March 2010
Methanol poisoning as a new world challenge: A review Nekoukar, Zahra; Zakariaei, Zakaria; Taghizadeh, Fatemeh Annals of Medicine & Surgery, Vol. 66 https://doi.org/10.1016/j.amsu.2021.102445	journal	June 2021
Evaluation of the quantity and composition of sugars and lipid in the juice and bagasse of lipid producing sugarcane Huang, Haibo; Moreau, Robert A.; Powell, Michael J. Biocatalysis and Agricultural Biotechnology, Vol. 10 https://doi.org/10.1016/j.bcab.2017.03.003	journal	April 2017
Hydrothermal pretreatment enhanced enzymatic hydrolysis and glucose production from oil palm biomass Zakaria, Mohd Rafein; Hirata, Satoshi; Hassan, Mohd Ali Bioresource Technology, Vol. 176 https://doi.org/10.1016/j.biortech.2014.11.027	journal	January 2015
Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects Jönsson, Leif J.; Martín, Carlos Bioresource Technology, Vol. 199 https://doi.org/10.1016/j.biortech.2015.10.009	journal	January 2016
Effect of severity factor on the hydrothermal pretreatment of sugarcane straw Batista, Gustavo; Souza, Renata B. A.; Pratto, Bruna Bioresource Technology, Vol. 275 https://doi.org/10.1016/j.biortech.2018.12.073	journal	March 2019
Engineering aspects of hydrothermal pretreatment: From batch to continuous operation, scale-up and pilot reactor under biorefinery concept Ruiz, Héctor A.; Conrad, Marc; Sun, Shao-Ni Bioresource Technology, Vol. 299 https://doi.org/10.1016/j.biortech.2019.122685	journal	March 2020
Balancing sugar recovery and inhibitor generation during energycane processing: Coupling cryogenic grinding with hydrothermal pretreatment at low temperatures Maitra, Shraddha; Singh, Vijay Bioresource Technology, Vol. 321 https://doi.org/10.1016/j.biortech.2020.124424	journal	February 2021
Chemical-free production of multiple high-value bioproducts from metabolically engineered transgenic sugarcane ‘oilcane’ bagasse and their recovery using nanofiltration Jia, Yuyao; Maitra, Shraddha; Singh, Vijay Bioresource Technology, Vol. 371 https://doi.org/10.1016/j.biortech.2023.128630	journal	March 2023
Scale-up of microbial lipid and bioethanol production from oilcane Deshavath, Narendra Naik; Woodruff, William; Eller, Fred Bioresource Technology, Vol. 399 https://doi.org/10.1016/j.biortech.2024.130594	journal	May 2024
Phaffia rhodozyma biorefinery: A sustainable pathway to obtain natural pigments and production of methane biogas as renewable fuel Mussagy, Cassamo U.; dos Santos, Amanda; F. G. Militão, Gabriely Chemical Engineering Journal, Vol. 473 https://doi.org/10.1016/j.cej.2023.145350	journal	October 2023
Pilot-scale processing of Miscanthus x giganteus for recovery of anthocyanins integrated with production of microbial lipids and lignin-rich residue Banerjee, Shivali; Dien, Bruce S.; Eilts, Kristen K. Chemical Engineering Journal, Vol. 485 https://doi.org/10.1016/j.cej.2024.150117	journal	April 2024
Application of natural deep eutectic solvents to the extraction of anthocyanins from Catharanthus roseus with high extractability and stability replacing conventional organic solvents Dai, Yuntao; Rozema, Evelien; Verpoorte, Robert Journal of Chromatography A, Vol. 1434 https://doi.org/10.1016/j.chroma.2016.01.037	journal	February 2016
Sustainable bioprocess technologies for urban waste valorization Banerjee, Shivali; Arora, Amit Case Studies in Chemical and Environmental Engineering, Vol. 4 https://doi.org/10.1016/j.cscee.2021.100166	journal	December 2021
The biorefinery concept: Using biomass instead of oil for producing energy and chemicals Cherubini, Francesco Energy Conversion and Management, Vol. 51, Issue 7 https://doi.org/10.1016/j.enconman.2010.01.015	journal	July 2010
Bioenergy crops as alternative feedstocks for recovery of anthocyanins: A review Banerjee, Shivali; Singh, Ramkrishna; Singh, Vijay Environmental Technology & Innovation, Vol. 29 https://doi.org/10.1016/j.eti.2022.102977	journal	February 2023
Phytochemical contents and antioxidant capacities of different parts of two sugarcane (Saccharum officinarum L.) cultivars Feng, Simin; Luo, Zisheng; Zhang, Yanbing Food Chemistry, Vol. 151 https://doi.org/10.1016/j.foodchem.2013.11.057	journal	May 2014
Bioactivity and anthocyanin content of microwave-assisted subcritical water extracts of Manipur black rice (Chakhao) bran and straw Moirangthem, Kamaljit; Ramakrishna, Priya; Amer, Mahetab H. Future Foods, Vol. 3 https://doi.org/10.1016/j.fufo.2021.100030	journal	June 2021
Separation of anthocyanins extracted from red cabbage by adsorption onto chitosan films Carvalho, Valéria V. L.; Gonçalves, Janaína O.; Silva, Adriano International Journal of Biological Macromolecules, Vol. 131 https://doi.org/10.1016/j.ijbiomac.2019.03.145	journal	June 2019
Cultivation of medicinal and aromatic plants for specialty industrial materials Lubbe, Andrea; Verpoorte, Robert Industrial Crops and Products, Vol. 34, Issue 1 https://doi.org/10.1016/j.indcrop.2011.01.019	journal	July 2011
Anthocyanins characterization and antioxidant activities of sugarcane (Saccharum officinarum L.) rind extracts Zhao, Zhengang; Yan, Huaifeng; Zheng, Rui Industrial Crops and Products, Vol. 113 https://doi.org/10.1016/j.indcrop.2018.01.015	journal	March 2018
Improving technical and economic feasibility of water based anthocyanin recovery from purple corn using staged extraction approach Kurambhatti, Chinmay; Kumar, Deepak; Rausch, Kent D. Industrial Crops and Products, Vol. 158 https://doi.org/10.1016/j.indcrop.2020.112976	journal	December 2020
Natural deep eutectic solvents (NADES) assisted deconstruction of oilcane bagasse for high lipid and sugar recovery Raj, Tirath; Singh, Vijay Industrial Crops and Products, Vol. 210 https://doi.org/10.1016/j.indcrop.2024.118127	journal	April 2024
Valorization of Miscanthus x giganteus for sustainable recovery of anthocyanins and enhanced production of sugars Banerjee, Shivali; Singh, Ramkrishna; Eilts, Kristen Journal of Cleaner Production, Vol. 369 https://doi.org/10.1016/j.jclepro.2022.133508	journal	October 2022
A consolidated bioprocess design to produce multiple high-value platform chemicals from lignocellulosic biomass and its technoeconomic feasibility Maitra, Shraddha; Singh, Vijay Journal of Cleaner Production, Vol. 377 https://doi.org/10.1016/j.jclepro.2022.134383	journal	December 2022
Citric acid water-based solution for blueberry bagasse anthocyanins recovery: Optimization and comparisons with microwave-assisted extraction (MAE). Ferreira, Lauren Fresinghelli; Minuzzi, Natalia Machado; Rodrigues, Renata Fritzsche LWT, Vol. 133 https://doi.org/10.1016/j.lwt.2020.110064	journal	November 2020
Effect of thermal processing on anthocyanin stability in foods; mechanisms and kinetics of degradation Patras, Ankit; Brunton, Nigel. P.; O'Donnell, Colm Trends in Food Science & Technology, Vol. 21, Issue 1 https://doi.org/10.1016/j.tifs.2009.07.004	journal	January 2010
Co-Processing Agricultural Residues and Wet Organic Waste Can Produce Lower-Cost Carbon-Negative Fuels and Bioplastics Wang, Yan; Baral, Nawa R.; Yang, Minliang Environmental Science & Technology, Vol. 57, Issue 7 https://doi.org/10.1021/acs.est.2c06674	journal	February 2023
Novel Approaches in the Valorization of Agricultural Wastes and Their Applications Capanoglu, Esra; Nemli, Elifsu; Tomas-Barberan, Francisco Journal of Agricultural and Food Chemistry, Vol. 70, Issue 23 https://doi.org/10.1021/acs.jafc.1c07104	journal	February 2022
Bioprocessing, Recovery, and Mass Balance of Vegetative Lipids from Metabolically Engineered “Oilcane” Demonstrates Its Potential as an Alternative Feedstock for Drop-In Fuel Production Maitra, Shraddha; Viswanathan, Mothi Bharath; Park, Kiyoul ACS Sustainable Chemistry & Engineering, Vol. 10, Issue 50 https://doi.org/10.1021/acssuschemeng.2c05327	journal	December 2022
Uncommonly High Levels of 3-Deoxyanthocyanidins and Antioxidant Capacity in the Leaf Sheaths of Dye Sorghum Kayodé, A. P. Polycarpe; Nout, M. J. Rob; Linnemann, Anita R. Journal of Agricultural and Food Chemistry, Vol. 59, Issue 4 https://doi.org/10.1021/jf103963t	journal	January 2011
Mutagenesis Breeding for Increased 3-Deoxyanthocyanidin Accumulation in Leaves of Sorghum bicolor (L.) Moench: A Source of Natural Food Pigment Petti, Carloalberto; Kushwaha, Rekha; Tateno, Mizuki Journal of Agricultural and Food Chemistry, Vol. 62, Issue 6 https://doi.org/10.1021/jf405324j	journal	February 2014
Anthocyanins Contents, Profiles, and Color Characteristics of Red Cabbage Extracts from Different Cultivars and Maturity Stages Ahmadiani, Neda; Robbins, Rebecca J.; Collins, Thomas M. Journal of Agricultural and Food Chemistry, Vol. 62, Issue 30 https://doi.org/10.1021/jf501991q	journal	July 2014
Subcritical water extraction of bioactive compounds from Orostachys japonicus A. Berger (Crassulaceae) Ko, Min-Jung; Nam, Hwa-Hyun; Chung, Myong-Soo Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-67508-2	journal	July 2020
The seeds of green energy: Expanding the contribution of plant oils as biofuels Ohlrogge, John; Chapman, Kent The Biochemist, Vol. 33, Issue 2 https://doi.org/10.1042/BIO03302034	journal	April 2011
Integrated Biorefinery for Valorization of Engineered Bioenergy Crops—A Review Singh, Ramkrishna; Singh, Vijay Industrial Biotechnology, Vol. 17, Issue 5 https://doi.org/10.1089/ind.2021.0020	journal	October 2021
Determination of Total Monomeric Anthocyanin Pigment Content of Fruit Juices, Beverages, Natural Colorants, and Wines by the pH Differential Method: Collaborative Study Lee, Jungmin; Durst, Robert W.; Wrolstad, Ronald E. Journal of AOAC INTERNATIONAL, Vol. 88, Issue 5 https://doi.org/10.1093/jaoac/88.5.1269	journal	September 2005
Towards oilcane: Engineering hyperaccumulation of triacylglycerol into sugarcane stems Parajuli, Saroj; Kannan, Baskaran; Karan, Ratna GCB Bioenergy, Vol. 12, Issue 7 https://doi.org/10.1111/gcbb.12684	journal	May 2020
Extraction of anthocyanins from grape pomace by using supercritical carbon dioxide Pazir, Fikret; Koçak, Erinç; Turan, Funda Journal of Food Processing and Preservation, Vol. 45, Issue 8 https://doi.org/10.1111/jfpp.14950	journal	November 2020
Metabolic engineering of sugarcane to accumulate energy-dense triacylglycerols in vegetative biomass Zale, Janice; Jung, Je Hyeong; Kim, Jae Yoon Plant Biotechnology Journal, Vol. 14, Issue 2 https://doi.org/10.1111/pbi.12411	journal	June 2015
Up-regulation of lipid biosynthesis increases the oil content in leaves of Sorghum bicolor Vanhercke, Thomas; Belide, Srinivas; Taylor, Matthew C. Plant Biotechnology Journal, Vol. 17, Issue 1 https://doi.org/10.1111/pbi.12959	journal	July 2018
Metabolic engineering of energycane to hyperaccumulate lipids in vegetative biomass Luo, Guangbin; Cao, Viet Dang; Kannan, Baskaran BMC Biotechnology, Vol. 22, Issue 1 https://doi.org/10.1186/s12896-022-00753-7	journal	August 2022
Microbiome differences in sugarcane and metabolically engineered oilcane accessions and their implications for bioenergy production Yang, Jihoon; Sooksa-nguan, Thanwalee; Kannan, Baskaran Biotechnology for Biofuels and Bioproducts, Vol. 16, Issue 1 https://doi.org/10.1186/s13068-023-02302-6	journal	March 2023
Hydrothermal Pretreatment of Lignocellulosic Feedstocks to Facilitate Biochemical Conversion Martín, Carlos; Dixit, Pooja; Momayez, Forough Frontiers in Bioengineering and Biotechnology, Vol. 10 https://doi.org/10.3389/fbioe.2022.846592	journal	February 2022
Optimizing Chemical-Free Pretreatment for Maximizing Oil/Lipid Recovery From Transgenic Bioenergy Crops and Its Rapid Analysis Using Time Domain-NMR Maitra, Shraddha; Long, Stephen P.; Singh, Vijay Frontiers in Energy Research, Vol. 10 https://doi.org/10.3389/fenrg.2022.840418	journal	April 2022
Anthocyanins: Factors Affecting Their Stability and Degradation Enaru, Bianca; Drețcanu, Georgiana; Pop, Teodora Daria Antioxidants, Vol. 10, Issue 12 https://doi.org/10.3390/antiox10121967	journal	December 2021
Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods Slama, Houda Ben; Chenari Bouket, Ali; Pourhassan, Zeinab Applied Sciences, Vol. 11, Issue 14 https://doi.org/10.3390/app11146255	journal	July 2021
Novel Insights into Anthocyanin Metabolism and Molecular Characterization of Associated Genes in Sugarcane Rinds Using the Metabolome and Transcriptome Rao, Muhammad Junaid; Duan, Mingzheng; Yang, Mingchong International Journal of Molecular Sciences, Vol. 23, Issue 1 https://doi.org/10.3390/ijms23010338	journal	December 2021
Plant-Derived Colorants for Food, Cosmetic and Textile Industries: A Review Brudzyńska, Patrycja; Sionkowska, Alina; Grisel, Michel Materials, Vol. 14, Issue 13 https://doi.org/10.3390/ma14133484	journal	June 2021
Optimization of a Sustainable Protocol for the Extraction of Anthocyanins as Textile Dyes from Plant Materials Gecchele, Elisa; Negri, Stefano; Cauzzi, Anna Molecules, Vol. 26, Issue 22 https://doi.org/10.3390/molecules26226775	journal	November 2021
Extraction Solvents Affect Anthocyanin Yield, Color, and Profile of Strawberries Taghavi, Toktam; Patel, Hiral; Rafie, Reza Plants, Vol. 12, Issue 9 https://doi.org/10.3390/plants12091833	journal	April 2023
Optimization of Red Pigment Anthocyanin Recovery from Hibiscus sabdariffa by Subcritical Water Extraction Rizkiyah, Dwila Nur; Putra, Nicky Rahmana; Idham, Zuhaili Processes, Vol. 10, Issue 12 https://doi.org/10.3390/pr10122635	journal	December 2022
Recovery of Anthocyanins from Hibiscus sabdariffa L. Using a Combination of Supercritical Carbon Dioxide Extraction and Subcritical Water Extraction Rizkiyah, Dwila Nur; Putra, Nicky Rahmana; Idham, Zuhaili Processes, Vol. 11, Issue 3 https://doi.org/10.3390/pr11030751	journal	March 2023
HPLC-UV-ESI-MS methods for flavonoid profiling of sugarcane juice extract Zhao, Yi; Zhu, Licai; Yu, Shujuan Sugar Industry https://doi.org/10.36961/si14371	journal	January 2013

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