Fate of the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) in soil and bioaccumulation in bush bean hydroponic plants

Journal Article · Mon Jul 01 00:00:00 EDT 1991 · Environmental Toxicology and Chemistry

DOI:https://doi.org/10.1002/etc.5620100701· OSTI ID:2496781

Harvey, Scott D.; Fellows, Robert J.; Cataldo, Dominic A.; Bean, Roger M.

Abstract

Soils amended with [14C]hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) were sampled over 60 d and subjected to exhaustive Soxhlet extraction followed by HPLC analysis. RDX was the only radiolabeled compound observed in soil extracts. Emission of volatile organics and 14CO2 from soil accounted for only 0.31% of the amended radiolabel. Mass balance for RDX-amended soil was better than 84% throughout the two-month study. The analytical method developed for plants involved acid hydrolysis, solvent extraction, fractionation on Florisil® adsorbent and separation by HPLC. The described methodology allowed for RDX recovery of 86 ± 3% from fortified bush bean leaf tissue. Further experiments were conducted with bush bean plants maintained on RDX-containing hydroponic solutions. Hydroponic plants did not emit detectable amounts of 14CO2 or radiolabeled volatile organics. Analysis of the plant tissue indicated bioaccumulation of RDX in the aerial tissues of hydroponic plants exposed for either 1 or 7 d. Metabolism of RDX to polar metabolites was observed in plants exposed for 7 d.

• Sponsoring Organization: USDOE
• Grant/Contract Number: AC06-76RL01830
• OSTI ID: 2496781
• Alternate ID(s): OSTI ID: 6911999
• Journal Information: Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Journal Issue: 7 Vol. 10; ISSN 0730-7268
• Publisher: Oxford University Press
• Country of Publication: United States
• Language: English