Glycolysis and krebs cycle metabolites in mosquitofish, Gambusia holbrooki , Girard 1859, exposed to mercuric chloride: Allozyme genotype effects

Newman, Michael C.; Mulvey, Margaret; Kramer, Vincent J.; Ultsch, Gordon R.

doi:10.1002/etc.5620110309

Glycolysis and krebs cycle metabolites in mosquitofish, Gambusia holbrooki , Girard 1859, exposed to mercuric chloride: Allozyme genotype effects

Journal Article · Sun Mar 01 00:00:00 EST 1992 · Environmental Toxicology and Chemistry

DOI:https://doi.org/10.1002/etc.5620110309· OSTI ID:2496741

Newman, Michael C.; Mulvey, Margaret; Kramer, Vincent J.; Ultsch, Gordon R.

Abstract

Concentrations of glycolysis and Krebs (citric acid) cycle metabolites were measured in the tail tissues of mosquitofish (Gambusia holbrooki, Girard 1859) in response to exposure to 0.86 mg/L Hg (as HgCl2) for 28 h. Substrate and product concentrations were compared between allozyme genotypes at two loci (glucosephosphate isomerase-2 and malate dehydrogenase-1) to determine whether allozyme genotypes in mosquitofish were differentially inhibited by mercury. Mercury treatment, regardless of allozyme genotype, caused decreased concentrations of glucose-6-phosphate (-27%) and lactate (-27%). Mercury treatment led to increased concentrations of malate (+33%) and oxaloacetate (+28%). Increased Krebs cycle activity could have been a response to greater energy needs associated with maintaining homeostasis under stressful conditions. There was no evidence of differential inhibition of allozymes at either locus. Fish of genotype Gpi-238/38 exhibited an overall increase in glycolytic activity in response to mercury treatment.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC09-76SR00819

OSTI ID:: 2496741

Journal Information:: Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Journal Issue: 3 Vol. 11; ISSN 0730-7268

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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