RNA Processing Bodies, Peroxisomes, Golgi Bodies, Mitochondria, and Endoplasmic Reticulum Tubule Junctions Frequently Pause at Cortical Microtubules

Hamada, Takahiro; Tominaga, Motoki; Fukaya, Takashi; Nakamura, Masayoshi; Nakano, Akihiko; Watanabe, Yuichiro; Hashimoto, Takashi; Baskin, Tobias I.

doi:10.1093/pcp/pcs025

RNA Processing Bodies, Peroxisomes, Golgi Bodies, Mitochondria, and Endoplasmic Reticulum Tubule Junctions Frequently Pause at Cortical Microtubules

Journal Article · Thu Mar 01 00:00:00 EST 2012 · Plant and Cell Physiology

DOI:https://doi.org/10.1093/pcp/pcs025· OSTI ID:2483301

Hamada, Takahiro ^[1]; Tominaga, Motoki ^[2]; Fukaya, Takashi ^[3]; Nakamura, Masayoshi ^[4]; Nakano, Akihiko ^[5]; Watanabe, Yuichiro ^[3]; Hashimoto, Takashi ^[4]; Baskin, Tobias I. ^[6]

University of Massachusetts, Amherst, MA (United States); Nara Institute of Science and Technology, Ikoma (Japan); Kyoto University (Japan)
RIKEN Advanced Science Institute, Saitama (Japan)
University of Tokyo (Japan)
Nara Institute of Science and Technology, Ikoma (Japan)
RIKEN Advanced Science Institute, Saitama (Japan); University of Tokyo (Japan)
University of Massachusetts, Amherst, MA (United States)

Organelle motility, essential for cellular function, is driven by the cytoskeleton. In plants, actin filaments sustain the long-distance transport of many types of organelles, and microtubules typically fine-tune the motile behavior. In shoot epidermal cells of Arabidopsis thaliana seedlings, we show here that a type of RNA granule, the RNA processing body (P-body), is transported by actin filaments and pauses at cortical microtubules. Interestingly, removal of microtubules does not change the frequency of P-body pausing. Similarly, we show that Golgi bodies, peroxisomes, and mitochondria all pause at microtubules, and again the frequency of pauses is not appreciably changed after microtubules are depolymerized. To understand the basis for pausing, we examined the endoplasmic reticulum (ER), whose overall architecture depends on actin filaments. By the dual observation of ER and microtubules, we find that stable junctions of tubular ER occur mainly at microtubules. Removal of microtubules reduces the number of stable ER tubule junctions, but those remaining are maintained without microtubules. The results indicate that pausing on microtubules is a common attribute of motile organelles but that microtubules are not required for pausing. Here, we suggest that pausing on microtubules facilitates interactions between the ER and otherwise translocating organelles in the cell cortex.

View Accepted Manuscript (DOE)

Research Organization:: University of Massachusetts, Amherst, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: FG02-03ER15421

OSTI ID:: 2483301

Journal Information:: Plant and Cell Physiology, Journal Name: Plant and Cell Physiology Journal Issue: 4 Vol. 53; ISSN 0032-0781

Publisher:: Japanese Society of Plant PhysiologistsCopyright Statement

Country of Publication:: United States

Language:: English

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RNA Processing Bodies, Peroxisomes, Golgi Bodies, Mitochondria, and Endoplasmic Reticulum Tubule Junctions Frequently Pause at Cortical Microtubules

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