Cytosolic proteins from tobacco pollen tubes that crosslink microtubules and actin filaments in vitro are metabolic enzymes

Romagnoli, Silvia; Faleri, Claudia; Bini, Luca; Baskin, Tobias I.; Cresti, Mauro

doi:10.1002/cm.20483

Cytosolic proteins from tobacco pollen tubes that crosslink microtubules and actin filaments in vitro are metabolic enzymes

Journal Article · Wed Sep 22 00:00:00 EDT 2010 · Cytoskeleton

DOI:https://doi.org/10.1002/cm.20483· OSTI ID:2483299

Romagnoli, Silvia ^[1]; Faleri, Claudia ^[2]; Bini, Luca ^[2]; Baskin, Tobias I. ^[3]; Cresti, Mauro ^[2]

Università di Siena (Italy); UMass
Università di Siena (Italy)
University of Massachusetts, Amherst, MA (United States)

In plant cells, many processes require cooperative action of both microtubules and actin filaments, but proteins mediating interactions between these cytoskeletal members are mostly undiscovered. Here, we attempt to identify such proteins by affinity purification. Cytosol from Nicotiana tabacum (tobacco) pollen tubes was incubated first with actin filaments, and then proteins eluted from the actin were incubated with microtubules, and finally those microtubule-binding proteins were pooled in an active fraction. This fraction bundled actin filaments but not microtubules. However, when the fraction was added to both actin and microtubules, large bundles resulted, containing both polymers, regardless of the order of addition of components. Similar results were obtained when the order of affinity purification was reversed. The four most abundant bands from the fractions were identified from peptide fragments analyzed by mass spectrometry. The same four proteins were identified regardless of the order of affinity purification. The proteins are: homocysteine methyltransferase, phosphofructokinase, pyruvate decarboxylase, and glucan protein synthase (reversibly glycosylated protein). Furthermore, these results suggest the importance of structuring metabolism within the confines of the pollen tube cytoplasm.

Research Organization:: University of Massachusetts, Amherst, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

DOE Contract Number:: FG02-03ER15421

OSTI ID:: 2483299

Journal Information:: Cytoskeleton, Journal Name: Cytoskeleton Journal Issue: 12 Vol. 67; ISSN 1949-3584

Publisher:: Wiley

Country of Publication:: United States

Language:: English

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Nicotiana tabacum
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Cytosolic proteins from tobacco pollen tubes that crosslink microtubules and actin filaments in vitro are metabolic enzymes

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