Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Cortical Microtubule Arrays Lose Uniform Alignment Between Cells and are Oryzalin Resistant in the Arabidopsis Mutant, radially swollen 6

Journal Article · · Plant and Cell Physiology
DOI:https://doi.org/10.1093/pcp/pcj067· OSTI ID:2483288
 [1];  [2];  [3];  [4];  [5]
  1. University of Massachusetts, Amherst, MA (United States); University of Sydney, NSW (Australia)
  2. University of Missouri, Columbia, MO (United States)
  3. Australian National University (Australia)
  4. University of Sydney, NSW (Australia)
  5. University of Massachusetts, Amherst, MA (United States)
+ Show Author Affiliations
The coordinated expansion of cells is essential to the formation of correctly shaped plant tissues and organs. Members of the radially swollen (rsw) class of temperature- sensitive arabidopsis mutants were isolated in a screen for reduced anisotropic expansion, by selecting plants with radially swollen root tips. Here we describe rsw6, in which cortical microtubules in the root epidermis are well organized in parallel arrays within cells, but neighboring cells frequently contain arrays differing in their mean orientation by up to 908. Microtubules in rsw6 are more resistant to oryzalin- induced depolymerization than wild-type microtubules, and their reorientation is accompanied by swelling of the epidermal cells. The reorientation phenotype is blocked by taxol and by the depolymerization of actin filaments. Here, we propose that rsw6 microtubule organization is functional on a local level, but defective on a global scale. The rsw6 mutant provides a unique tool with which to study the coordination of microtubule organization at a multicellular level.
Research Organization:
University of Massachusetts, Amherst, MA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB); United States National Science Foundation (NSF)
Grant/Contract Number:
FG02-03ER15421
OSTI ID:
2483288
Journal Information:
Plant and Cell Physiology, Journal Name: Plant and Cell Physiology Journal Issue: 7 Vol. 47; ISSN 0032-0781
Publisher:
Japanese Society of Plant PhysiologistsCopyright Statement
Country of Publication:
United States
Language:
English

References (42)

Elucidating the mechanism of cortical microtubule reorientation in plant cells journal January 1996
How I learned to love carrots: the role of the cytoskeleton in shaping plant cells journal December 1999
Reorganization of cortical microtubules and cellulose deposition during leaf formation in Graptopetalum paraguayense journal July 1980
Changes in microfibril arrangement on the inner surface of the epidermal cell walls in the epicotyl of Vigna angularis ohwi et ohashi during cell growth journal April 1981
Arrangement of cortical microtubules in the shoot apex of Vinca major L. journal September 1988
Morphology ofrsw1, a cellulose-deficient mutant ofArabidopsis thaliana journal March 2001
On the alignment of cellulose microfibrils by cortical microtubules: A review and a model journal March 2001
A new method for immunofluorescent localization of microtubules in surface cell layers: application to the shoot apical meristem ofHedera journal June 1989
Intercellular Alignments of the Plant Cytoskeleton journal June 2001
The reb1-1 mutation of Arabidopsis alters the morphology of trichoblasts, the expression of arabinogalactan-proteins and the organization of cortical microtubules journal October 2002
The cyclic reorientation of cortical microtubules in epidermal cells of azuki bean epicotyls: the role of actin filaments in the progression of the cycle journal June 1998
Dinitroaniline herbicide resistance and the microtubule cytoskeleton journal March 1999
Arabidopsis Interdigitating Cell Growth Requires Two Antagonistic Pathways with Opposing Action on Cell Morphogenesis journal March 2005
Progress in understanding the role of microtubules in plant cells journal December 2004
Directional cell expansion — turning toward actin journal December 2005
MOR1 is essential for organizing cortical microtubules in plants journal May 2001
Normal differentiation patterns in plants lacking microtubular preprophase bands journal June 1995
Microtubule basis for left-handed helical growth in Arabidopsis journal May 2002
Plasma membrane depolarization‐activated calcium channels, stimulated by microtubule‐depolymerizing drugs in wild‐type Arabidopsis thaliana protoplasts, display constitutively large activities and a longer half‐life in ton 2 mutant cells affected in the organization of cortical microtubules journal March 1998
BOTERO1 is required for normal orientation of cortical microtubules and anisotropic cell expansion in Arabidopsis journal January 2001
Root Morphology Mutants in Arabidopsis thaliana journal January 1992
Disruption of Actin Microfilaments Causes Cortical Microtubule Disorganization and Extra-Phragmoplast Formation at M/G1 Interface in Synchronized Tobacco Cells journal June 2004
Actin Control Over Microtubules Suggested by DISTORTED2 Encoding the Arabidopsis ARPC2 Subunit Homolog journal July 2004
Plant-Specific Microtubule-Associated Protein SPIRAL2 Is Required for Anisotropic Growth in Arabidopsis journal November 2004
Inhibitors of Protein Kinases and Phosphatases Alter Root Morphology and Disorganize Cortical Microtubules journal February 1997
The Mechanic State of “Inner Tissue” in the Growing Zone of Sunflower Hypocotyls and the Regulation of Its Growth Rate Following Excision journal June 2000
Covalent Binding of the Benzamide RH-4032 to Tubulin in Suspension-Cultured Tobacco Cells and Its Application in a Cell-Based Competitive-Binding Assay journal September 2000
Temperature-Sensitive Alleles of RSW2 Link the KORRIGAN Endo-1,4-β-Glucanase to Cellulose Synthesis and Cytokinesis in Arabidopsis journal May 2001
Mutation or Drug-Dependent Microtubule Disruption Causes Radial Swelling without Altering Parallel Cellulose Microfibril Deposition in Arabidopsis Root Cells journal May 2003
A Semidominant Mutation in an Arabidopsis Mitogen-Activated Protein Kinase Phosphatase-Like Gene Compromises Cortical Microtubule Organization[W] journal July 2004
The Tangled1 Gene Is Required for Spatial Control of Cytoskeletal Arrays Associated with Cell Division during Maize Leaf Development journal November 1998
The Cortical Microtubule Array: From Dynamics to Organization journal October 2004
A Katanin-like Protein Regulates Normal Cell Wall gBiosynthesis and Cell Elongation journal April 2001
Sustained Microtubule Treadmilling in Arabidopsis Cortical Arrays journal June 2003
The effects of taxol and colchicine on microtubule and microfibril arrays in elongating plant cells in culture journal September 1988
Spatial Control of cell Expansion by the Plant Cytoskeleton journal November 2005
Organogenesis-A Biophysical View journal June 1980
Three Brick genes have distinct functions in a common pathway promoting polarized cell division and cell morphogenesis in the maize leaf epidermis journal February 2003
The SPIRAL genes are required for directional control of cell elongation in Aarabidopsis thaliana journal October 2000
Mutant alleles of Arabidopsis RADIALLY SWOLLEN 4 and 7 reduce growth anisotropy without altering the transverse orientation of cortical microtubules or cellulose microfibrils journal October 2002
Microtubule organization in the green kingdom: chaos or self-order? journal April 2002
Interphase microtubule re-orientation predicts a new cell polarity in wounded pea roots journal May 1990

Similar Records

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Cytoskeleton
Epidermis
Oryzalin
Radial expansion
Root
Taxol