Development of Enzyme Production Host (CRADA Final Report)
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Protein Evolution, Inc., New Haven, CT (United States)
This CRADA aims to assess the effectiveness of various enzyme production hosts in efficiently expressing and producing proprietary enzymes for plastic recycling applications. The research includes screening different microbial hosts—notably Bacillus subtilis, Pichia pastoris, and E. coli—to determine their suitability for high-yield enzyme production. Additionally, the project focuses on optimizing scalable fermentation processes tailored to these hosts, with the goal of enabling efficient enzyme production at commercially viable scales.
- Research Organization:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Protein Evolution, Inc., New Haven, CT (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- DOE Contract Number:
- AC02-05CH11231
- OSTI ID:
- 2480573
- Report Number(s):
- LBNL--2001619; AWD00007026; FP00016789
- Country of Publication:
- United States
- Language:
- English
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