Geranylgeranylated-chlorophyll-protein complexes in lhl3 mutant of the green alga Chlamydomonas reinhardtii

Kodru, Sireesha; Nellaepalli, Sreedhar; Ozawa, Shin‐Ichiro; Satoh, Chihiro; Kuroda, Hiroshi; Tanaka, Ryouichi; Guan, Katharine; Kobayashi, Marilyn; Tran, Phoi; McCarthy, Sarah; Wakao, Setsuko; Niyogi, Krishna K.; Takahashi, Yuichiro

doi:10.1111/tpj.17071

Geranylgeranylated-chlorophyll-protein complexes in lhl3 mutant of the green alga Chlamydomonas reinhardtii

Journal Article · Tue Oct 15 00:00:00 EDT 2024 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.17071· OSTI ID:2479281

Kodru, Sireesha ^[1]; Nellaepalli, Sreedhar ^[1]; Ozawa, Shin‐Ichiro ^[1]; Satoh, Chihiro ^[1]; Kuroda, Hiroshi ^[1]; ^[2]; Guan, Katharine ^[3]; Kobayashi, Marilyn ^[3]; Tran, Phoi ^[3]; McCarthy, Sarah ^[3]; Wakao, Setsuko ^[4]; Niyogi, Krishna K. ^[4]; ^[1]

Okayama University (Japan); JST‐CREST, Tokyo (Japan)
Hokkaido University, Sapporo (Japan)
University of California, Berkeley, CA (United States)
University of California, Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Chlorophylls a and b (Chl a and b) are involved in light harvesting, photochemical reactions, and electron transfer reactions in plants and green algae. The core complexes of the photosystems (PSI and PSII) associate with Chl a, while the peripheral antenna complexes (LHCI and LHCII) bind Chls a and b. One of the final steps of Chl biosynthesis is the conversion of geranylgeranylated Chls (Chls_GG) to phytylated Chls by geranylgeranyl reductase (GGR). Here, we isolated and characterized a pale green mutant of the green alga Chlamydomonas reinhardtii that was very photosensitive and was unable to grow photoautotrophically. This mutant has a 16-bp deletion in the LHL3 gene, which resulted in the loss of LHL3 and GGR and accumulated only Chls_GG. The lhl3 mutant cells grown in the dark accumulated PSII and PSI proteins at 25–50% of WT levels, lacked PSII activity, and retained a decreased PSI activity. The PSII and PSI proteins were depleted to trace amounts in the mutant cells grown in light. In contrast, the accumulation of LHCI and LHCII was unaffected except for LHCA3. Our results suggest that the replacement of Chls with Chls_GG strongly affects the structural and functional integrity of PSII and PSI complexes but their associating LHC complexes to a lesser extent. Affinity purification of HA-tagged LHL3 confirmed the formation of a stable LHL3-GGR complex, which is vital for GGR stability. The LHL3-GGR complex contained a small amount of PSI complex assembly factors, suggesting a putative coupling between Chl synthesis and PSI complex assembly.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: JSPS KAKENHI; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2479281

Journal Information:: The Plant Journal, Journal Name: The Plant Journal Journal Issue: 4 Vol. 120; ISSN 0960-7412

Publisher:: Society for Experimental BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Geranylgeranylated-chlorophyll-protein complexes in lhl3 mutant of the green alga Chlamydomonas reinhardtii

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