In Vitro Antimicrobial Activity of Volatile Compounds from the Lichen Pseudevernia furfuracea (L.) Zopf. Against Multidrug-Resistant Bacteria and Fish Pathogens

Essadki, Yasser; Hilmi, Adel; Cascajosa-Lira, Antonio; Girão, Mariana; Darrag, El Mehdi; Martins, Rosário; Romane, Abderrahmane; El Amrani Zerrifi, Soukaina; Mugani, Richard; Tazart, Zakaria; Redouane, El Mahdi; Jos, Angeles; Cameán, Ana M.; Vasconcelos, Vitor; Campos, Alexandre; El Khalloufi, Fatima; Oudra, Brahim; Barakate, Mustapha; Carvalho, Maria de Fátima

doi:10.3390/microorganisms12112336

In Vitro Antimicrobial Activity of Volatile Compounds from the Lichen Pseudevernia furfuracea (L.) Zopf. Against Multidrug-Resistant Bacteria and Fish Pathogens

Journal Article · Fri Nov 15 00:00:00 EST 2024 · Microorganisms

DOI:https://doi.org/10.3390/microorganisms12112336· OSTI ID:2477852

; Hilmi, Adel; ; ; ; ; Romane, Abderrahmane; El Amrani Zerrifi, Soukaina; ; ; Redouane, El Mahdi; ; ; ; ; ; ; ;

Lichens are symbiotic organisms with unique secondary metabolism. Various metabolites from lichens have shown antimicrobial activity. Nevertheless, very few studies have investigated the antimicrobial potential of the volatile compounds they produce. This study investigates the chemical composition and antimicrobial properties of volatile compounds from Pseudevernia furfuracea collected in two regions of Morocco. Hydrodistillation was used to obtain volatile compounds from samples collected in the High Atlas and Middle Atlas. Gas chromatography–mass spectrometry (GC-MS) analysis identified phenolic cyclic compounds as the primary constituents, with atraric acid and chloroatranol being the most abundant. Additionally, eight compounds were detected in lichens for the first time. The antimicrobial activity of these compounds was assessed using disc diffusion and broth microdilution methods. Both samples demonstrated significant antimicrobial effects against multidrug-resistant human bacteria, reference microorganisms, fish pathogens, and Candida albicans, with minimum inhibitory concentrations (MICs) ranging from 1000 µg/mL to 31.25 µg/mL. This study provides the first report on the volatile compounds from Pseudevernia furfuracea and their antimicrobial effects, particularly against fish pathogens, suggesting their potential as novel antimicrobial agents for human and veterinary use. Further research is warranted to explore these findings in more detail.

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Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Fuel Cell Technologies Office

OSTI ID:: 2477852

Journal Information:: Microorganisms, Journal Name: Microorganisms Journal Issue: 11 Vol. 12; ISSN 2076-2607; ISSN MICRKN

Publisher:: MDPI AGCopyright Statement

Country of Publication:: Switzerland

Language:: English

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