Human Liver Epithelium Response to HCoV-229E Infection Epigenomics (ACS-DP4)

Dataset · Wed Sep 25 04:00:00 EDT 2024

DOI:https://doi.org/10.25584/PPI/2475746· OSTI ID:2475746

Sims, Amy C ^[1]; Leiser, Owen P ^[2]; Anderson, Lindsey N ^[2]; Waters, Katrina M ^[2]

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1. Pacific Northwest National Laboratory (PNNL); PNNL
2. Pacific Northwest National Laboratory (PNNL)

The purpose of this experiment was to evaluate how wild-type Human coronavirus strain 229E (HCoV-229E) infection alters chromatin accessibility in infected cells only. Sample data was obtained for mock and infected (standard and UV-inactivated) immortalized human liver cells (HuH-7) and collected 24 hrs. post infection. Samples were processed using assay for transposase-accessible chromatin using high-throughput sequencing (ATAC-Seq) and generated bar coded library samples were evaluated for RNA sequencing (RNA-Seq) expression analysis. Processed ATAC-Seq datasets are openly accessible from the download button and contain secondary processed RNA-Seq results files and supporting metadata materials. Data download includes a sample naming key, infection titer metadata, normalized counts, and relevant computational source code information supporting data transparency and reuse.

• Research Organization: Pacific Northwest National Laboratory 2; PNNL
• Sponsoring Organization: SC
• Contributing Organization: Predictive Phenomics Initiative, Pacific Northwest National Laboratory
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 2475746
• Report Number(s): PNNL-SA-204870; 30487
• Availability: [e-mail]
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Predictive Phenomics
ATAC-Seq
Lethal Human Viruses
HCov-229E
Homo sapiens
Epigenomics