Targeted modulation of pennycress lipid droplet proteins impacts droplet morphology and seed oil content

Guzha, Athanas; Gautam, Barsanti; Marchiafava, Damiano; Ver Sagun, Julius; Garcia, Tatiana; Jarvis, Brice A.; Barbaglia‐Hurlock, Allison M.; Johnston, Christopher; Grotewold, Erich; Sedbrook, John C.; Alonso, Ana Paula; Chapman, Kent D.

doi:10.1111/tpj.17109

Targeted modulation of pennycress lipid droplet proteins impacts droplet morphology and seed oil content

Journal Article · Mon Oct 28 00:00:00 EDT 2024 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.17109· OSTI ID:2475222

Guzha, Athanas ^[1]; Gautam, Barsanti ^[2]; Marchiafava, Damiano ^[2]; Ver Sagun, Julius ^[1]; Garcia, Tatiana ^[3]; Jarvis, Brice A. ^[2]; Barbaglia‐Hurlock, Allison M. ^[3]; Johnston, Christopher ^[1]; ^[3]; Sedbrook, John C. ^[2]; Alonso, Ana Paula ^[1]; ^[1]

BioDiscovery Institute Department of Biological Sciences University of North Texas Denton 76203 Texas USA
School of Biological Sciences Illinois State University Normal Illinois USA
Department of Biochemistry and Molecular Biology Michigan State University East Lansing Michigan USA

SUMMARY

Lipid droplets (LDs) are unusual organelles that have a phospholipid monolayer surface and a hydrophobic matrix. In oilseeds, this matrix is nearly always composed of triacylglycerols (TGs) for efficient storage of carbon and energy. Various proteins play a role in their assembly, stability and turnover, and even though the major structural oleosin proteins in seed LDs have been known for decades, the factors influencing LD formation and dynamics are still being uncovered mostly in the “model oilseed” Arabidopsis. Here we identified several key LD biogenesis proteins in the seeds of pennycress, a potential biofuel crop, that were correlated previously with seed oil content and characterized here for their participation in LD formation in transient expression assays and stable transgenics. One pennycress protein, the lipid droplet associated protein‐interacting protein (LDIP), was able to functionally complement the Arabidopsis ldip mutant, emphasizing the close conservation of lipid storage among these two Brassicas. Moreover, loss‐of‐function ldip mutants in pennycress exhibited increased seed oil content without compromising plant growth, raising the possibility that LDIP or other LD biogenesis factors may be suitable targets for improving yields in oilseed crops more broadly.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0020325; SC0021286

OSTI ID:: 2475222

Journal Information:: The Plant Journal, Journal Name: The Plant Journal Journal Issue: 5 Vol. 120; ISSN 0960-7412

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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