DNA Origami Incorporated into Solid-State Nanopores Enables Enhanced Sensitivity for Precise Analysis of Protein Translocations

Joty, Kamruzzaman; Ghimire, Madhav L.; Kahn, Jason S.; Lee, Sangyoup; Alexandrakis, George; Kim, Min Jun

doi:10.1021/acs.analchem.4c02016

DNA Origami Incorporated into Solid-State Nanopores Enables Enhanced Sensitivity for Precise Analysis of Protein Translocations

Journal Article · Fri Oct 18 00:00:00 EDT 2024 · Analytical Chemistry

DOI:https://doi.org/10.1021/acs.analchem.4c02016· OSTI ID:2473074

^[1]; Ghimire, Madhav L. ^[1]; ^[2]; ^[3]; ^[4]; ^[1]

Southern Methodist Univ., Dallas, TX (United States)
Brookhaven National Laboratory (BNL), Upton, NY (United States). Center for Functional Nanomaterials (CFN)
Korea Institute of Science and Technology (KIST) (Korea, Republic of)
Univ. of Texas, Arlington, TX (United States)

The rapidly advancing field of nanotechnology is driving the development of precise sensing methods at the nanoscale, with solid-state nanopores emerging as promising tools for biomolecular sensing. Here, this study investigates the increased sensitivity of solid-state nanopores achieved by integrating DNA origami structures, leading to the improved analysis of protein translocations. Using holo human serum transferrin (holo-hSTf) as a model protein, we compared hybrid nanopores incorporating DNA origami with open solid-state nanopores. Results show a significant enhancement in holo-hSTf detection sensitivity with DNA origami integration, suggesting a unique role of DNA interactions beyond confinement. This approach holds potential for ultrasensitive protein detection in biosensing applications, offering advancements in biomedical research and diagnostic tool development for diseases with low-abundance protein biomarkers. Further exploration of origami designs and nanopore configurations promises even greater sensitivity and versatility in the detection of a wider range of proteins, paving the way for advanced biosensing technologies.

View Accepted Manuscript (DOE)

Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF)

Grant/Contract Number:: SC0012704; SC0008772

OSTI ID:: 2473074

Report Number(s):: BNL--226277-2024-JAAM

Journal Information:: Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 44 Vol. 96; ISSN 0003-2700

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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DNA Origami Incorporated into Solid-State Nanopores Enables Enhanced Sensitivity for Precise Analysis of Protein Translocations

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