Compositional and temporal division of labor modulates mixed sugar fermentation by an engineered yeast consortium

Shin, Jonghyeok; Liao, Siqi; Kuanyshev, Nurzhan; Xin, Yongping; Kim, Chanwoo; Lu, Ting; Jin, Yong-Su

doi:10.1038/s41467-024-45011-w

Compositional and temporal division of labor modulates mixed sugar fermentation by an engineered yeast consortium

Journal Article · Fri Jan 26 00:00:00 EST 2024 · Nature Communications

DOI:https://doi.org/10.1038/s41467-024-45011-w· OSTI ID:2282924

; ; ; ; Kim, Chanwoo; ;

Abstract

Synthetic microbial communities have emerged as an attractive route for chemical bioprocessing. They are argued to be superior to single strains through microbial division of labor (DOL), but the exact mechanism by which DOL confers advantages remains unclear. Here, we utilize a synthetic Saccharomyces cerevisiae consortium along with mathematical modeling to achieve tunable mixed sugar fermentation to overcome the limitations of single-strain fermentation. The consortium involves two strains with each specializing in glucose or xylose utilization for ethanol production. By controlling initial community composition, DOL allows fine tuning of fermentation dynamics and product generation. By altering inoculation delay, DOL provides additional programmability to parallelly regulate fermentation characteristics and product yield. Mathematical models capture observed experimental findings and further offer guidance for subsequent fermentation optimization. This study demonstrates the functional potential of DOL in bioprocessing and provides insight into the rational design of engineered ecosystems for various applications.

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Research Organization:: University of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: Korea Research Institute of Bioscience and Biotechnology (KRIBB); USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0019185

OSTI ID:: 2282924

Alternate ID(s):: OSTI ID: 2472274

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 15; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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