Analysis of 26S Proteasome Activity across Arabidopsis Tissues

Ganapathy, Jagadeesan; Hand, Katherine A.; Shabek, Nitzan

doi:10.3390/plants13121696

Analysis of 26S Proteasome Activity across Arabidopsis Tissues

Journal Article · Wed Jun 19 00:00:00 EDT 2024 · Plants

DOI:https://doi.org/10.3390/plants13121696· OSTI ID:2472201

Ganapathy, Jagadeesan ^[1]; ^[2]; ^[2]

University of California, Davis, CA (United States); OSTI
University of California, Davis, CA (United States)

Plants utilize the ubiquitin proteasome system (UPS) to orchestrate numerous essential cellular processes, including the rapid responses required to cope with abiotic and biotic stresses. The 26S proteasome serves as the central catalytic component of the UPS that allows for the proteolytic degradation of ubiquitin-conjugated proteins in a highly specific manner. Despite the increasing number of studies employing cell-free degradation assays to dissect the pathways and target substrates of the UPS, the precise extraction methods of highly potent tissues remain unexplored. Here, we utilize a fluorogenic reporting assay using two extraction methods to survey proteasomal activity in different Arabidopsis thaliana tissues. This study provides new insights into the enrichment of activity and varied presence of proteasomes in specific plant tissues.

View Accepted Manuscript (DOE)

Research Organization:: University of California, Davis, CA (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0023158

OSTI ID:: 2472201

Journal Information:: Plants, Journal Name: Plants Journal Issue: 12 Vol. 13; ISSN 2223-7747

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

References (31)

Spatial distribution of the 26S proteasome in meristematic tissues and primordia of rice ( Oryza sativa L.) Yanagawa, Yuki; Kimura, Seisuke; Takase, Tomomasa Planta, Vol. 214, Issue 5 https://doi.org/10.1007/s00425-001-0676-2	journal	March 2002
Mass spectrometric analysis of expression of ATPase subunits encoded by duplicated genes in the 19S regulatory particle of rice 26S proteasome Shibahara, Tadashi; Kawasaki, Hiroshi; Hirano, Hisashi Archives of Biochemistry and Biophysics, Vol. 421, Issue 1 https://doi.org/10.1016/j.abb.2003.10.013	journal	January 2004
Structure, function and regulation of plant proteasomes Kurepa, Jasmina; Smalle, Jan A. Biochimie, Vol. 90, Issue 2 https://doi.org/10.1016/j.biochi.2007.07.019	journal	February 2008
Plant Signaling: Ubiquitin Pulls the Trigger on Chloroplast Degradation Ling, Qihua; Jarvis, Paul Current Biology, Vol. 26, Issue 1 https://doi.org/10.1016/j.cub.2015.11.022	journal	January 2016
LEAFY homeostasis is regulated via ubiquitin-dependent degradation and sequestration in cytoplasmic condensates Dolde, Ulla; Muzzopappa, Fernando; Delesalle, Charlotte iScience, Vol. 26, Issue 6 https://doi.org/10.1016/j.isci.2023.106880	journal	June 2023
Mechanism of Gate Opening in the 20S Proteasome by the Proteasomal ATPases Rabl, Julius; Smith, David M.; Yu, Yadong Molecular Cell, Vol. 30, Issue 3 https://doi.org/10.1016/j.molcel.2008.03.004	journal	May 2008
Ubiquitination in plant immunity Trujillo, Marco; Shirasu, Ken Current Opinion in Plant Biology, Vol. 13, Issue 4 https://doi.org/10.1016/j.pbi.2010.04.002	journal	August 2010
Ubiquitination in the control of photoperiodic flowering Piñeiro, Manuel; Jarillo, José A. Plant Science, Vol. 198 https://doi.org/10.1016/j.plantsci.2012.10.005	journal	January 2013
Cryo-EM structure of the plant 26S proteasome Kandolf, Susanne; Grishkovskaya, Irina; Belačić, Katarina Plant Communications, Vol. 3, Issue 3 https://doi.org/10.1016/j.xplc.2022.100310	journal	May 2022
The life cycle of the 26S proteasome: from birth, through regulation and function, and onto its death Livneh, Ido; Cohen-Kaplan, Victoria; Cohen-Rosenzweig, Chen Cell Research, Vol. 26, Issue 8 https://doi.org/10.1038/cr.2016.86	journal	July 2016
Allosteric coupling between α-rings of the 20S proteasome Yu, Zanlin; Yu, Yadong; Wang, Feng Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-18415-7	journal	September 2020
A conformational switch in the SCF-D3/MAX2 ubiquitin ligase facilitates strigolactone signalling Tal, Lior; Palayam, Malathy; Ron, Mily Nature Plants, Vol. 8, Issue 5 https://doi.org/10.1038/s41477-022-01145-7	journal	April 2022
Structural plasticity of D3–D14 ubiquitin ligase in strigolactone signalling Shabek, Nitzan; Ticchiarelli, Fabrizio; Mao, Haibin Nature, Vol. 563, Issue 7733 https://doi.org/10.1038/s41586-018-0743-5	journal	November 2018
Purification of the Arabidopsis 26 S Proteasome : BIOCHEMICAL AND MOLECULAR ANALYSES REVEALED THE PRESENCE OF MULTIPLE ISOFORMS Yang, Peizhen; Fu, Hongyong; Walker, Joseph Journal of Biological Chemistry, Vol. 279, Issue 8 https://doi.org/10.1074/jbc.M311977200	journal	November 2003
The ubiquitin system affects agronomic plant traits Linden, Katrina J.; Callis, Judy Journal of Biological Chemistry, Vol. 295, Issue 40 https://doi.org/10.1074/jbc.REV120.011303	journal	October 2020
The 26S proteasome: A cell cycle regulator regulated by cell cycle Guo, Xing; Dixon, Jack E. Cell Cycle, Vol. 15, Issue 7 https://doi.org/10.1080/15384101.2016.1151728	journal	March 2016
C‐terminal conformational changes in SCF‐D3/MAX2 ubiquitin ligase are required for KAI2‐mediated signaling Tal, Lior; Guercio, Angelica M.; Varshney, Kartikye New Phytologist, Vol. 239, Issue 6 https://doi.org/10.1111/nph.19101	journal	June 2023
The ubiquitin‐proteasome system in plant responses to environments Xu, Fa‐Qing; Xue, Hong‐Wei Plant, Cell & Environment, Vol. 42, Issue 10 https://doi.org/10.1111/pce.13633	journal	August 2019
A Practical Review of Proteasome Pharmacology Thibaudeau, Tiffany A.; Smith, David M. Pharmacological Reviews, Vol. 71, Issue 2 https://doi.org/10.1124/pr.117.015370	journal	March 2019
Structure and Function of the 26S Proteasome Bard, Jared A. M.; Goodall, Ellen A.; Greene, Eric R. Annual Review of Biochemistry, Vol. 87, Issue 1 https://doi.org/10.1146/annurev-biochem-062917-011931	journal	June 2018
Principles of Ubiquitin-Dependent Signaling Oh, Eugene; Akopian, David; Rape, Michael Annual Review of Cell and Developmental Biology, Vol. 34, Issue 1 https://doi.org/10.1146/annurev-cellbio-100617-062802	journal	October 2018
The Ubiquitin System Hershko, Avram; Ciechanover, Aaron Annual Review of Biochemistry, Vol. 67, Issue 1 https://doi.org/10.1146/annurev.biochem.67.1.425	journal	June 1998
The 26S Proteasome: A Molecular Machine Designed for Controlled Proteolysis Voges, D.; Zwickl, P.; Baumeister, W. Annual Review of Biochemistry, Vol. 68, Issue 1 https://doi.org/10.1146/annurev.biochem.68.1.1015	journal	June 1999
Recognition and Processing of Ubiquitin-Protein Conjugates by the Proteasome Finley, Daniel Annual Review of Biochemistry, Vol. 78, Issue 1 https://doi.org/10.1146/annurev.biochem.78.081507.101607	journal	June 2009
Protein Degradation by the Ubiquitin–Proteasome Pathway in Normal and Disease States Lecker, Stewart H.; Goldberg, Alfred L.; Mitch, William E. Journal of the American Society of Nephrology, Vol. 17, Issue 7 https://doi.org/10.1681/ASN.2006010083	journal	May 2006
Highlighting the Proteasome: Using Fluorescence to Visualize Proteasome Activity and Distribution Gan, Jin; Leestemaker, Yves; Sapmaz, Aysegul Frontiers in Molecular Biosciences, Vol. 6 https://doi.org/10.3389/fmolb.2019.00014	journal	March 2019
Dynamic Regulation of the 26S Proteasome: From Synthesis to Degradation Marshall, Richard S.; Vierstra, Richard D. Frontiers in Molecular Biosciences, Vol. 6 https://doi.org/10.3389/fmolb.2019.00040	journal	June 2019
The Role of Anaphase-Promoting Complex/Cyclosome (APC/C) in Plant Reproduction Saleme, Marina de Lyra Soriano; Andrade, Ingrid Rocha; Eloy, Nubia Barbosa Frontiers in Plant Science, Vol. 12 https://doi.org/10.3389/fpls.2021.642934	journal	February 2021
Advances on Plant Ubiquitylome—From Mechanism to Application He, Dongli; Damaris, Rebecca Njeri; Li, Ming International Journal of Molecular Sciences, Vol. 21, Issue 21 https://doi.org/10.3390/ijms21217909	journal	October 2020
Degradation of ubiquitin: The fate of the cellular reaper Shabek, Nitzan; Ciechanover, Aaron Cell Cycle, Vol. 9, Issue 3 https://doi.org/10.4161/cc.9.3.11152	journal	February 2010
Proteasome regulation, plant growth and stress tolerance Kurepa, Jasmina; Wang, Songhu; Li, Yan Plant Signaling & Behavior, Vol. 4, Issue 10 https://doi.org/10.4161/psb.4.10.9469	journal	October 2009

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59 BASIC BIOLOGICAL SCIENCES
cell-free system
fluorogenic probes
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plant sciences
protein degradation
proteolytic activity
ubiquitin system

Analysis of 26S Proteasome Activity across Arabidopsis Tissues

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References (31)

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