Reclassification of Botryococcus braunii chemical races into separate species based on a comparative genomics analysis

Boland, Devon J.; Cornejo-Corona, Ivette; Browne, Daniel R.; Murphy, Rebecca L.; Mullet, John; Okada, Shigeru; Devarenne, Timothy P.

doi:10.1371/journal.pone.0304144

Reclassification of Botryococcus braunii chemical races into separate species based on a comparative genomics analysis

Journal Article · Mon Jul 29 00:00:00 EDT 2024 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0304144· OSTI ID:2471862

^[1]; ^[2]; Browne, Daniel R. ^[3]; ^[4]; Mullet, John ^[2]; Okada, Shigeru ^[5]; ^[2]

Texas A & M University, College Station, TX (United States); OSTI
Texas A & M University, College Station, TX (United States)
Texas A & M University, College Station, TX (United States); LanzaTech Inc., Skokie, IL (United States)
Texas A & M University, College Station, TX (United States); Centenary College of Louisiana, Shreveport, LA (United States)
The University of Tokyo, Bunkyo (Japan)

The colonial green microalga Botryococcus braunii is well known for producing liquid hydrocarbons that can be utilized as biofuel feedstocks. B. braunii is taxonomically classified as a single species made up of three chemical races, A, B, and L, that are mainly distinguished by the hydrocarbons produced. We previously reported a B race draft nuclear genome, and here we report the draft nuclear genomes for the A and L races. A comparative genomic study of the three B. braunii races and 14 other algal species within Chlorophyta revealed significant differences in the genomes of each race of B. braunii. Phylogenomically, there was a clear divergence of the three races with the A race diverging earlier than both the B and L races, and the B and L races diverging from a later common ancestor not shared by the A race. DNA repeat content analysis suggested the B race had more repeat content than the A or L races. Orthogroup analysis revealed the B. braunii races displayed more gene orthogroup diversity than three closely related Chlamydomonas species, with nearly 24-36% of all genes in each B. braunii race being specific to each race. This analysis suggests the three races are distinct species based on sufficient differences in their respective genomes. We propose reclassification of the three chemical races to the following species names: Botryococcus alkenealis (A race), Botryococcus braunii (B race), and Botryococcus lycopadienor (L race).

View Accepted Manuscript (DOE)

Research Organization:: Donald Danforth Plant Science Center, St. Louis, MO (United States)

Sponsoring Organization:: JSPS KAKENHI; National Science Foundation (NSF); USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: EE0003046

OSTI ID:: 2471862

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 7 Vol. 19; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Oil accumulation mechanisms of the oleaginous microalga Chlorella protothecoides revealed through its genome, transcriptomes, and proteomes Gao, Chunfang; Wang, Yun; Shen, Yue BMC Genomics, Vol. 15, Issue 1 https://doi.org/10.1186/1471-2164-15-582	journal	January 2014
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Elucidation of the Potential Hair Growth-Promoting Effect of Botryococcus terribilis, Its Novel Compound Methylated-Meijicoccene, and C32 Botryococcene on Cultured Hair Follicle Dermal Papilla Cells Using DNA Microarray Gene Expression Analysis Oliva, Aprill Kee; Bejaoui, Meriem; Hirano, Atsushi Biomedicines, Vol. 10, Issue 5 https://doi.org/10.3390/biomedicines10051186	journal	May 2022
Lineage-Specific Genes and Family Expansions in Dictyostelid Genomes Display Expression Bias and Evolutionary Diversification during Development Luna, Saara K.; Chain, Frédéric J. J. Genes, Vol. 12, Issue 10 https://doi.org/10.3390/genes12101628	journal	October 2021
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Hydrocarbons
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