Generating Novel Aroma Phenotypes Using Commercial Wine Samples to Characterize an F1 Population

Awale, Mani; Liu, Connie; Kwasniewski, Misha T.

doi:10.3389/fpls.2022.894492

Generating Novel Aroma Phenotypes Using Commercial Wine Samples to Characterize an F1 Population

Journal Article · Tue Jun 21 00:00:00 EDT 2022 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2022.894492· OSTI ID:2471385

Awale, Mani ^[1]; Liu, Connie ^[2]; Kwasniewski, Misha T. ^[3]

Pennsylvania State Univ., University Park, PA (United States); Univ. of Missouri, Columbia, MO (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Univ. of Missouri, Columbia, MO (United States)
Pennsylvania State Univ., University Park, PA (United States); Univ. of Missouri, Columbia, MO (United States)

Due to their disease tolerance and cold hardy nature, interspecific hybrid grapes are widely grown in the Midwestern and Northeastern United States, with additional interest worldwide in the face of increased abiotic and biotic stresses from climate change. However, the aroma profile of these hybrids is unique and generally less popular in comparison withVitis viniferagrapes. One of the challenges in any phenotyping project is first defining the traits of interest. As wine quality was our ultimate metric of interest, the aroma profile of commercial wines produced from the parents of a breeding population (Vitis aestivalisderived ‘Norton’ xV. vinifera. ‘Cabernet Sauvignon’) was first assessed for traits of interest. We investigated 11 commercial wines each of Norton, a popular hybrid in Missouri and Cabernet Sauvignon (Cab) for their volatile profiles using the more inclusive metabolomics-based workflow. We then analyzed 21 Norton and 21 Cab grapes from different sites and vintages for the free and bound volatile compounds using HS-SPME-GCMS to validate the differences in wine. The GCMS data was processed using XCMS software to find features that were different between the two cultivars. The two cultivars were found to have differences in their volatile profiles, with 304 features different for wine volatiles, 418 features different for free volatiles, and 302 features different for bound volatiles at 0.05 significance level and with at least a 1.5-fold change between the two cultivars. Those features were used to identify several odor-active compounds in both grapes and wines, including β-damascenone, β-ionone, eugenol, 1,1,6-trimethyl-1,2-dihydronaphthalene (TDN), and methyl salicylate. Some of the identified compounds were higher in Norton than Cab; however, several features were higher in Cab. Using the identified aroma compounds as markers, we phenotyped an F1 population of Norton and Cab. The F1 population was found to be segregating for many aroma compounds with some genotypes demonstrating an even higher concentration of aroma volatiles than either of the parents. Ultimately, using commercially available samples paired with untargeted analysis proved to be an efficient way to determine phenotypes of interest for further analysis and may offer an easy way to choose potential parents with desired traits for breeding.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDA; USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 2471385

Journal Information:: Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Vol. 13; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
F1 population
GC-MS
aroma compounds
interspecific hybrid
phenotyping
untargeted metabolomics

Generating Novel Aroma Phenotypes Using Commercial Wine Samples to Characterize an F1 Population

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