Coinfection of Influenza A and B and Human OC43 Coronavirus in Normal Human Bronchial Epithelial Cells

Kim, JungHyun; Hickerson, Brady T.; Ilyushina, Natalia A.

doi:10.1111/irv.13279

Coinfection of Influenza A and B and Human OC43 Coronavirus in Normal Human Bronchial Epithelial Cells

Journal Article · Sun Mar 31 00:00:00 EDT 2024 · Influenza and Other Respiratory Viruses

DOI:https://doi.org/10.1111/irv.13279· OSTI ID:2471280

^[1]; Hickerson, Brady T. ^[1]; ^[1]

US Food and Drug Administration (USFDA), Silver Spring, MD (United States)

Influenza viruses and seasonal coronaviruses are pathogens transmitted via an airborne route that can cause respiratory diseases in humans that have similar symptoms such as fever, cough, and pneumonia. These two viruses can infect similar human tissues, such as the respiratory tract and nasal, bronchial, and alveolar epithelial cells. Influenza virus and seasonal coronavirus coinfections are poorly understood. Here, we coinfected normal human bronchial epithelial (NHBE) cells with influenza A/California/04/09 (IAV) or B/Victoria/504/2000 (IBV) strains and the seasonal human beta-coronavirus OC43 and evaluated viral replication capacities. We also examined changes in the expression of various cytokines/chemokines by qPCR and Luminex assay. We observed that the replication of IAV and IBV was not affected by coinfection with OC43. However, coinfection reduced OC43 titers (~3-fold) compared with infection with OC43 alone. Select cytokine/chemokine expression was increased in coinfected cells compared with all single infections with greater differences seen between coinfected cells and cells infected with OC43 alone compared with IAV- or IBV-infected cells. In addition, IL-8 and IL-1RA showed the highest expression among a panel of 22 cytokines by Luminex. As the rate of influenza and seasonal coronavirus coinfection continue to increase, our findings may help set guidelines for the treatments of the individuals coinfected with both viruses.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0014664

OSTI ID:: 2471280

Journal Information:: Influenza and Other Respiratory Viruses, Journal Name: Influenza and Other Respiratory Viruses Journal Issue: 4 Vol. 18; ISSN 1750-2640

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

60 APPLIED LIFE SCIENCES
Infectious Diseases
Virology
coinfection
cytokines
influenza
seasonal coronavirus

Coinfection of Influenza A and B and Human OC43 Coronavirus in Normal Human Bronchial Epithelial Cells

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