Proteomic Analysis of Pecan (Carya illinoinensis) Nut Development

Clermont, Kristen; Graham, Charles J.; Lloyd, Steven W.; Grimm, Casey C.; Randall, Jennifer J.; Mattison, Christopher P.

doi:10.3390/foods12040866

Proteomic Analysis of Pecan (Carya illinoinensis) Nut Development

Journal Article · Thu Feb 16 23:00:00 EST 2023 · Foods

DOI:https://doi.org/10.3390/foods12040866· OSTI ID:2471256

^[1]; Graham, Charles J. ^[2]; ^[3]; Grimm, Casey C. ^[3]; Randall, Jennifer J. ^[4]; ^[3]

U.S. Department of Agriculture, New Orleans, LA (United States); Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States); James Madison University, Harrisonburg, VA (United States)
Noble Research Institute, Ardmore, OK (United States)
U.S. Department of Agriculture, New Orleans, LA (United States)
New Mexico State University, Las Cruces, NM (United States)

Pecan (Carya illinoinensis) nuts are an economically valuable crop native to the United States and Mexico. A proteomic summary from two pecan cultivars at multiple time points was used to compare protein accumulation during pecan kernel development. Patterns of soluble protein accumulation were elucidated using qualitative gel-free and label-free mass-spectrometric proteomic analyses and quantitative (label-free) 2-D gel electrophoresis. Two-dimensional (2-D) gel electrophoresis distinguished a total of 1267 protein spots and shotgun proteomics identified 556 proteins. Rapid overall protein accumulation occurred in mid-September during the transition to the dough stage as the cotyledons enlarge within the kernel. Pecan allergens Car i 1 and Car i 2 were first observed to accumulate during the dough stage in late September. While overall protein accumulation increased, the presence of histones diminished during development. Twelve protein spots accumulated differentially based on 2-D gel analysis in the weeklong interval between the dough stage and the transition into a mature kernel, while eleven protein spots were differentially accumulated between the two cultivars. These results provide a foundation for more focused proteomic analyses of pecans that may be used in the future to identify proteins that are important for desirable traits, such as reduced allergen content, improved polyphenol or lipid content, increased tolerance to salinity, biotic stress, seed hardiness, and seed viability.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0014664

OSTI ID:: 2471256

Journal Information:: Foods, Journal Name: Foods Journal Issue: 4 Vol. 12; ISSN 2304-8158

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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Proteomic Analysis of Pecan (Carya illinoinensis) Nut Development

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