Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

The Preclinical Validation of 405 nm Light Parasiticidal Efficacy on Leishmania donovani in Ex Vivo Platelets in a Rag2-/- Mouse Model

Journal Article · · Microorganisms
 [1];  [1];  [1];  [1];  [2];  [2];  [2];  [2];  [1];  [1];  [1]
  1. US Food and Drug Administration (USFDA), Silver Spring, MD (United States)
  2. University of Strathclyde, Glasgow (United Kingdom)
+ Show Author Affiliations
Violet–blue light of 405 nm in the visible spectrum at a dose of 270 J/cm2 alone has been shown to be an effective microbicidal tool for inactivating several bacteria, HIV-1, and Trypanosoma cruzi in ex vivo plasma and platelets. Unlike chemical- and ultraviolet (UV)-based pathogen inactivation methods for plasma and platelet safety, 405 nm light is shown to be less toxic to host cells at light doses that are microbicidal. In this report, we evaluated the parasiticidal activity of a 405 nm light treatment on platelets spiked with the Leishmania donovani parasite. Following the light treatment, parasite viability was observed to be near zero in both low- and high-titer-spiked platelets relative to controls. Furthermore, to test the residual infectivity after inactivation in vivo, the light-treated low-titer L. donovani-spiked platelets were evaluated in an immunodeficient Rag2-/- mouse model and monitored for 9 weeks. The parasiticidal efficacy of 405 nm light was evident from the lack of a presence of parasites in the mice spleens. Parasiticidal activity was confirmed to be mediated through 405 nm light-induced reactive oxygen species (ROS), as quantitatively measured by a 2',7'-Dichlorodihydrofluorescein diacetate (H2DCFDA)-based assay. Overall, these results confirm the complete inactivation of L. donovani spiked in ex vivo platelets by 405 nm light treatment and exemplify the utility of the Rag2-/- mouse infection model for the preclinical validation of the parasiticidal efficacy of 405 nm light and this light-based technology as a potential PRT for ex vivo platelets.
Research Organization:
Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
SC0014664
OSTI ID:
2471214
Journal Information:
Microorganisms, Journal Name: Microorganisms Journal Issue: 2 Vol. 12; ISSN 2076-2607
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English

References (24)

Incidence of Endemic Human Cutaneous Leishmaniasis in the United States journal September 2018
Lethal effects of high-intensity violet 405-nm light on Saccharomyces cerevisiae, Candida albicans, and on dormant and germinating spores of Aspergillus niger journal July 2013
405 nm light technology for the inactivation of pathogens and its potential role for environmental disinfection and infection control journal September 2014
A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing journal July 2020
Production of leishmanin skin test antigen from Leishmania donovani for future reintroduction in the field journal November 2023
Centrin-deficient Leishmania mexicana confers protection against New World cutaneous leishmaniasis journal March 2022
Blue Light Eliminates Community-Acquired Methicillin-ResistantStaphylococcus aureusin Infected Mouse Skin Abrasions journal November 2013
White blood cell inactivation after treatment with riboflavin and ultraviolet light journal October 2010
Sporicidal Effects of High-Intensity 405 nm Visible Light on Endospore-Forming Bacteria journal August 2012
Violet‐blue 405‐nm Light‐based Photoinactivation for Pathogen Reduction of Human Plasma Provides Broad Antibacterial Efficacy Without Visible Degradation of Plasma Proteins journal January 2022
Pathogen reduction of whole blood: utility and feasibility journal September 2017
Blue Light Rescues Mice from Potentially Fatal Pseudomonas aeruginosa Burn Infection: Efficacy, Safety, and Mechanism of Action journal March 2013
Inactivation of Bacterial Pathogens following Exposure to Light from a 405-Nanometer Light-Emitting Diode Array journal April 2009
Pathogen Inactivation Technologies for Cellular Blood Components: an Update journal January 2014
The Mirasol Evaluation of Reduction in Infections Trial (MERIT): study protocol for a randomized controlled clinical trial journal April 2022
Revival of Leishmanization and Leishmanin journal March 2021
Interferon-γ-Producing CD4+ T Cells Drive Monocyte Activation in the Bone Marrow During Experimental Leishmania donovani Infection journal September 2021
Ultraviolet-Based Pathogen Inactivation Systems: Untangling the Molecular Targets Activated in Platelets journal May 2018
Non-ionizing 405 nm Light as a Potential Bactericidal Technology for Platelet Safety: Evaluation of in vitro Bacterial Inactivation and in vivo Platelet Recovery in Severe Combined Immunodeficient Mice journal January 2020
Complete Inactivation of Blood Borne Pathogen Trypanosoma cruzi in Stored Human Platelet Concentrates and Plasma Treated With 405 nm Violet-Blue Light journal November 2020
Visible 405 nm Violet-Blue Light Successfully Inactivates HIV-1 in Human Plasma journal July 2022
Emerging Pathogen Threats in Transfusion Medicine: Improving Safety and Confidence with Pathogen Reduction Technologies journal July 2023
Cutting Edge: STAT1 and T-bet Play Distinct Roles in Determining Outcome of Visceral Leishmaniasis Caused by Leishmania donovani journal June 2006
Evaluation of bacterial inactivation in random donor platelets and single-donor apheresis platelets by the INTERCEPT blood system journal January 2018

Similar Records

Complete Inactivation of Blood Borne Pathogen Trypanosoma cruzi in Stored Human Platelet Concentrates and Plasma Treated With 405 nm Violet-Blue Light
Journal Article · Mon Nov 23 19:00:00 EST 2020 · Frontiers in Medicine · OSTI ID:1817079
Ly6Chi inflammatory monocytes promote susceptibility to Leishmania donovani infection
Journal Article · Mon Oct 30 20:00:00 EDT 2017 · Scientific Reports · OSTI ID:1624360
The circulating immunoglobulins negatively impact on the parasite clearance in the liver of Leishmania donovani-infected mice via dampening ROS activity
Journal Article · Wed Nov 14 23:00:00 EST 2018 · Biochemical and Biophysical Research Communications · OSTI ID:23127368

Related Subjects

405 nm light
59 BASIC BIOLOGICAL SCIENCES
Leishmania
microbiology
pathogen reduction
platelets
transfusion safety
transfusion transmissible parasites