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Luteibacter mycovicinus sp. nov., a yellow-pigmented gammaproteobacterium found as an endohyphal symbiont of endophytic Ascomycota

Journal Article · · International Journal of Systematic and Evolutionary Microbiology
 [1];  [2];  [1];  [1];  [3];  [4];  [1]
  1. University of Arizona, Tucson, AZ (United States)
  2. University of Arizona, Tucson, AZ (United States); University of North Carolina at Charlotte, NC (United States)
  3. University of Arizona, Tucson, AZ (United States); Hexagon Bio, Menlo Park, CA (United States)
  4. Indigo Agriculture, Boston, MA (United States)
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We isolated and described a yellow-pigmented strain of bacteria (strain 9143T), originally characterized as an endohyphal inhabitant of an endophytic fungus in the Ascomycota. Although the full-length sequence of its 16S rRNA gene displays 99 % similarity to Luteibacter pinisoli, genomic hybridization demonstrated <30 % genomic similarity between 9143T and its closest named relatives, further supported by average nucleotide identity results. This and related endohyphal strains form a well-supported clade separate from L. pinisoli and other validly named species including the most closely related Luteibacter rhizovicinus. The name Luteibacter mycovicinus sp. nov. is proposed, with type strain 9143T (isolate DBL433), for which a genome has been sequenced and is publicly available from the American Type Culture Collection (ATCC TSD-257T) and from the Leibniz Institute DSMZ (DSM 112764T). The type strain reliably forms yellow colonies across diverse media and growth conditions (lysogeny broth agar, King’s Medium B, potato dextrose agar, trypticase soy agar and Reasoner's 2A (R2A) agar). It forms colonies readily at 27 °C on agar with a pH of 6–8, and on salt (NaCl) concentrations up to 2 %. It lacks the ability to utilize sulphate as a sulphur source and thus only forms colonies on minimal media if supplemented with alternative sulphur sources. It is catalase-positive and oxidase-negative. Although it exhibits a single polar flagellum, motility was only clearly visible on R2A agar. Its host range and close relatives, which share the endohyphal lifestyle, are discussed.

Research Organization:
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); NSF Postdoctoral Research Fellowship
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
2471100
Journal Information:
International Journal of Systematic and Evolutionary Microbiology, Journal Name: International Journal of Systematic and Evolutionary Microbiology Journal Issue: 5 Vol. 74; ISSN 1466-5026
Publisher:
International Union of Microbiological SocietiesCopyright Statement
Country of Publication:
United States
Language:
English

References (25)

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EHB
Endohyphal bacteria
Endophyte
Luteibacter mycovicinus
Microbiology