Viral Capsid Change upon Encapsulation of Double-Stranded DNA into an Infectious Hypodermal and Hematopoietic Necrosis Virus-like Particle

Weerachatyanukul, Wattana; Kiatmetha, Pauline; Raksat, Ponlawoot; Boonkua, Supawich; Thongsum, Orawan; Jariyapong, Pitchanee; Chotwiwatthanakun, Charoonroj; Ounjai, Puey; Metlagel, Zoltan

doi:10.3390/v15010110

Viral Capsid Change upon Encapsulation of Double-Stranded DNA into an Infectious Hypodermal and Hematopoietic Necrosis Virus-like Particle

Journal Article · Fri Dec 30 00:00:00 EST 2022 · Viruses

DOI:https://doi.org/10.3390/v15010110· OSTI ID:2470796

Weerachatyanukul, Wattana ^[1]; Kiatmetha, Pauline ^[1]; Raksat, Ponlawoot ^[1]; ^[1]; Thongsum, Orawan ^[1]; Jariyapong, Pitchanee ^[2]; ^[3]; Ounjai, Puey ^[1]; Metlagel, Zoltan ^[4]

Mahidol University, Bangkok (Thailand)
Walailak University, Nakhonsrithammarat (Thailand)
Mahidol University, Nakhonsawan (Thailand)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); University of California, Berkeley, CA (United States)

In this study, we aimed to encapsulate the sizable double-stranded DNA (dsDNA, 3.9 kbp) into a small-sized infectious hypodermal and hematopoietic necrosis virus-like particle (IHHNV-VLP; T = 1) and compared the changes in capsid structure between dsDNA-filled VLP and empty VLP. Based on our encapsulation protocol, IHHNV-VLP was able to load dsDNA at an efficiency of 30–40% (w/w) into its cavity. Structural analysis revealed two subclasses of IHHNV-VLP, so-called empty and dsDNA-filled VLPs. The three-dimensional (3D) structure of the empty VLP produced in E. coli was similar to that of the empty IHHNV-VLP produced in Sf9 insect cells. The size of the dsDNA-filled VLP was slightly bigger (50 Å) than its empty VLP counterpart; however, the capsid structure was drastically altered. The capsid was about 1.5-fold thicker due to the thickening of the capsid interior, presumably from DNA–capsid interaction evident from capsid protrusions or nodules on the interior surface. In addition, the morphological changes of the capsid exterior were particularly observed in the vicinity of the five-fold axes, where the counter-clockwise twisting of the “tripod” structure at the vertex of the five-fold channel was evident, resulting in a widening of the channel’s opening. Whether these capsid changes are similar to virion capsid maturation in the host cells remains to be investigated. Nevertheless, the ability of IHHNV-VLP to encapsulate the sizable dsDNA has opened up the opportunity to package a dsDNA vector that can insert exogenous genes and target susceptible shrimp cells in order to halt viral infection.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: National Research Council of Thailand; Thailand Research Fund; USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2470796

Journal Information:: Viruses, Journal Name: Viruses Journal Issue: 1 Vol. 15; ISSN 1999-4915

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
IHHNV
Virology
capsid maturation
dsDNA
encapsulation
virus-like particles

Viral Capsid Change upon Encapsulation of Double-Stranded DNA into an Infectious Hypodermal and Hematopoietic Necrosis Virus-like Particle

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