Enzyme Complexes of Ptr4CL and PtrHCT Modulate Co-enzyme A Ligation of Hydroxycinnamic Acids for Monolignol Biosynthesis in Populus trichocarpa

Lin, Chien-Yuan; Sun, Yi; Song, Jina; Chen, Hsi-Chuan; Shi, Rui; Yang, Chenmin; Liu, Jie; Tunlaya-Anukit, Sermsawat; Liu, Baoguang; Loziuk, Philip L.; Williams, Cranos M.; Muddiman, David C.; Lin, Ying-Chung Jimmy; Sederoff, Ronald R.; Wang, Jack P.; Chiang, Vincent L.

doi:10.3389/fpls.2021.727932

Enzyme Complexes of Ptr4CL and PtrHCT Modulate Co-enzyme A Ligation of Hydroxycinnamic Acids for Monolignol Biosynthesis in Populus trichocarpa

Journal Article · Tue Oct 05 00:00:00 EDT 2021 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2021.727932· OSTI ID:2470758

Lin, Chien-Yuan ^[1]; Sun, Yi ^[2]; Song, Jina ^[3]; Chen, Hsi-Chuan ^[3]; Shi, Rui ^[3]; Yang, Chenmin ^[3]; Liu, Jie ^[3]; Tunlaya-Anukit, Sermsawat ^[3]; Liu, Baoguang ^[4]; Loziuk, Philip L. ^[3]; Williams, Cranos M. ^[3]; Muddiman, David C. ^[3]; Lin, Ying-Chung Jimmy ^[5]; Sederoff, Ronald R. ^[3]; Wang, Jack P. ^[5]; Chiang, Vincent L. ^[5]

North Carolina State University, Raleigh, NC (United States); Joint BioEnergy Institute (JBEI), Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Northeast Forestry University, Harbin (China)
North Carolina State University, Raleigh, NC (United States)
Northeast Forestry University, Harbin (China); Beihua University, Jilin (China)
North Carolina State University, Raleigh, NC (United States); Northeast Forestry University, Harbin (China)

Co-enzyme A (CoA) ligation of hydroxycinnamic acids by 4-coumaric acid:CoA ligase (4CL) is a critical step in the biosynthesis of monolignols. Perturbation of 4CL activity significantly impacts the lignin content of diverse plant species. In Populus trichocarpa, two well-studied xylem-specific Ptr4CLs (Ptr4CL3 and Ptr4CL5) catalyze the CoA ligation of 4-coumaric acid to 4-coumaroyl-CoA and caffeic acid to caffeoyl-CoA. Subsequently, two 4-hydroxycinnamoyl-CoA:shikimic acid hydroxycinnamoyl transferases (PtrHCT1 and PtrHCT6) mediate the conversion of 4-coumaroyl-CoA to caffeoyl-CoA. Here, we show that the CoA ligation of 4-coumaric and caffeic acids is modulated by Ptr4CL/PtrHCT protein complexes. Downregulation of PtrHCTs reduced Ptr4CL activities in the stem-differentiating xylem (SDX) of transgenic P. trichocarpa. The Ptr4CL/PtrHCT interactions were then validated in vivo using biomolecular fluorescence complementation (BiFC) and protein pull-down assays in P. trichocarpa SDX extracts. Enzyme activity assays using recombinant proteins of Ptr4CL and PtrHCT showed elevated CoA ligation activity for Ptr4CL when supplemented with PtrHCT. Numerical analyses based on an evolutionary computation of the CoA ligation activity estimated the stoichiometry of the protein complex to consist of one Ptr4CL and two PtrHCTs, which was experimentally confirmed by chemical cross-linking using SDX plant protein extracts and recombinant proteins. Based on these results, we propose that Ptr4CL/PtrHCT complexes modulate the metabolic flux of CoA ligation for monolignol biosynthesis during wood formation in P. trichocarpa.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2470758

Journal Information:: Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Vol. 12; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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