Pericytes control vascular stability and auditory spiral ganglion neuron survival

Zhang, Yunpei; Neng, Lingling; Sharma, Kushal; Hou, Zhiqiang; Johnson, Anatasiya; Song, Junha; Dabdoub, Alain; Shi, Xiaorui

doi:10.7554/elife.83486

Pericytes control vascular stability and auditory spiral ganglion neuron survival

Journal Article · Mon Jan 30 23:00:00 EST 2023 · eLife

DOI:https://doi.org/10.7554/elife.83486· OSTI ID:2470683

^[1]; Neng, Lingling ^[1]; Sharma, Kushal ^[1]; ^[1]; Johnson, Anatasiya ^[1]; Song, Junha ^[2]; ^[3]; ^[1]

Oregon Health & Science Univ., Portland, OR (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Univ. of Toronto, ON (Canada); Sunnybrook Research Institute, Toronto, ON (Canada)

The inner ear has a rich population of pericytes, a multi-functional mural cell essential for sensory hair cell heath and normal hearing. However, the mechanics of how pericytes contribute to the homeostasis of the auditory vascular-neuronal complex in the spiral ganglion are not yet known. In this study, using an inducible and conditional pericyte depletion mouse (PDGFRB-CreER^T2; ROSA26iDTR) model, we demonstrate, for the first time, that pericyte depletion causes loss of vascular volume and spiral ganglion neurons (SGNs) and adversely affects hearing sensitivity. Using an in vitro trans-well co-culture system, we show pericytes markedly promote neurite and vascular branch growth in neonatal SGN explants and adult SGNs. The pericyte-controlled neural growth is strongly mediated by pericyte-released exosomes containing vascular endothelial growth factor-A (VEGF-A). Treatment of neonatal SGN explants or adult SGNs with pericyte-derived exosomes significantly enhances angiogenesis, SGN survival, and neurite growth, all of which were inhibited by a selective blocker of VEGF receptor 2 (Flk1). Our study demonstrates that pericytes in the adult ear are critical for vascular stability and SGN health. Cross-talk between pericytes and SGNs via exosomes is essential for neuronal and vascular health and normal hearing.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2470683

Journal Information:: eLife, Journal Name: eLife Vol. 12; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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